Catalog # Aliquot Size P94-911C-05 3 x 5 nmol P94-911C-20 3 x 20 nmol P94-911C-50 3 x 50 nmol PDE6B siRNA Set I siRNA duplexes targeted against three exon regions Catalog # P94-911C Lot # Z2073-2 Specificity Formulation PDE6B siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PDE6B expression. shipped at room temperature. Product Description Reconstitution Protocol PDE6B siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PDE6B mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_000283. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot rd1; PDEB; RP40; CSNB3 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PDE6A, Active P94-31G below -20oC. After resuspension, siRNA stock solutions ≥2 PDE6B, Active P94-30BG µM can undergo up to 50 freeze-thaw cycles without PDE6C Protein P94-34CG significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background PDE6B is a member of the phosphodiesterase family of PDE6B siRNA Set I proteins that play a critical role in regulating intracellular siRNA duplexes targeted against three exon regions levels of cAMP and cGMP. PDE6B is a high-affinity cGMP- specific PDE that shows high expression in the eye. Four Catalog Number P94-911C mutations in the PDE6B gene lead to a degenerative Specific Lot Number Z2073-2 disease of photoreceptors called retinitis pigmentosa and Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol autosomal dominant congenital stationary night Format Lyophilized powder blindness (1). Subretinal injection of mice with retinal Stability 1yr at -70oC from date of shipment degeneration with PDE6B gene showed significant Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is decrease in photoreceptor cell death (2). A nonsense recommended that the lyophilized mutation in the PDE6B gene is also the cause of Rod- and resuspended siRNAs are stored at cone dysplasia-1 (rcd1) in Irish setters. or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles References without significant degradation. For 1. McLaughlin M E, et al: Recessive mutations in the long-term storage, it is recommended that the siRNA is stored at -70oC. For gene encoding the beta-subunit of rod most favorable performance, avoid phosphodiesterase in patients with retinitis repeated handling and multiple pigmentosa. Nat Genet. 1993 Jun;4(2):130-4. freeze/thaw cycles. 2. Bennett J, et al: Photoreceptor cell rescue in retinal degeneration (rd) mice by in vivo gene therapy. Nature Med. 2: 649, 1996. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .