Anti-Cryptosporidium Monoclonal antibody, Clone CFM 0237 (DCABY-030) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Specificity Mouse anti Cryptosporidium antibody, clone CFM 0237 recognizes a membrane antigen expressed by the oocysts of Cryptosporidium sp., an obligate enteric coccidian parasite of the phylum Apicomplexa that infects the gastrointestinal tract. Target Cryptosporidium Immunogen Purified cryptosporidium oocysts from bovine faeces. Isotype IgG3 Source/Host Mouse Species Reactivity Cryptosporidium Clone CFM 0237 Conjugate Unconjugated Applications ELISA, Flow Cytometry, Immunofluorescence Preparation Purified IgG prepared by affinity chromatography on Protein A Format Purified IgG - liquid Size 200 μg Buffer Phosphate buffered saline Preservative 0.09% Sodium Azide Storage Store at +4°C or at -20°C if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. BACKGROUND Introduction Cryptosporidium is a small protozoan parasite, measuring about 3-5 μm. It lives on (or just 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved under) the surface of the cells lining the small intestine, reproduces asexually, and oocysts are passed in the feces. Transmission of the infection occurs via the oocysts. Many human infections have been traced to the contamination of drinking water with oocysts from agricultural "run-off" (i.e., drainage from pastures), so it is considered a zoonosis. The most common symptom of cryptosporidiosis is watery diarrhea; other symptoms include stomach cramps or pain, fever, nausea and vomiting. In persons with healthy immune systems, symptoms usually last about 1 to3weeks. Keywords Cryptosporidium Parvum; C. parvum; Chromalveolata; Apicomplexa; Conoidasida; Coccidiasina; Eucoccidiorida; Cryptosporidiidae; Cryptosporidium GENE INFORMATION References 1. Mansfield, K.G. et al. (1997) Identification of an Enterocytozoon bieneusi-like microsporidian parasite in simian-immunodeficiency-virus-inoculated macaques with hepatobiliary disease.Am J Pathol. 150: 1395-405. 2. Meisel, J.L. et al. (1976) Overwhelming watery diarrhea associated with a cryptosporidium in an immunosuppressed patient. Gastroenterology. 70: 1156-60. 3. Rose, J.B. et al. (2002) Risk and control of waterborne cryptosporidiosis. FEMS Microbiol Rev. 26: 113-23. 4. Ma, P. (1984) Cryptosporidium and the enteropathy of immune deficiency.J. Pediatr. Gastroenterol. Nutr. 3: 488-90. 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.