Curriculum Vitae Name: Nathan E. Goldfarb, MS, PhD Address : 355 W 920 N A-308 Orem, UT 84057 Phone: (904) 716-7339 (cell) E-mail: [email protected] LinkedIn: http://www.linkedin.com/in/nathangoldfarb Impact of Recent First Author Publications: In the area of tUbercUlosis (TB) drUg discovery, Goldfarb, N.E. et al., PLOS Pathogens, 2014 unequivocally assign catalytic function to the TB drUg target, Hip1, a serine hydrolase that had previoUsly been erroneously reported to be devoid of proteolytic activity. We also validated that Hip1 indeed cleaves its proposed in vivo substrate, GroEL2. Based Upon the Hip1 recognition site in GroEL2, we designed the first flUorescent sUbstrate Useful for screening compoUnds for novel inhibitors of Hip1. These resUlts have profound implications on the preclinical drug discovery strategy utilized for this drug target; specifically, which class of inhibitors to screen for hit-to-lead optimization and which chemotypes to Utilize as warheads on Hip1 targeting seqUences for rational drUg design. In the realm of HIV drUg discovery, Goldfarb, N.E. et al., Biochemistry, 2015 provide the first experimental data illustrating a mechanism by which a distal mUtation in HIV protease transmits its effect over a long distance, in order to resUlt in weaker inhibitor binding in the active site of the protease. This mechanism describes a protease with an expanded active site and a conformational eqUilibriUm shifted to the open, active form. Aside from providing illUminating insights on general protein dynamics, this provides proof-of-concept for the design of bulkier inhibitors that will take Up the extra space in the active site and provides rational for the design of allosteric inhibitors that will shift the conformation of the protease back to the closed, inactive form. Education 2009-2015 Postdoctoral Associate/Fellow, laboratory of Ben M. DUnn, PhD, Department of Biochemistry and MolecUlar Biology University of Florida, Gainesville, Florida 2003-2005 Postdoctoral Fellowship Schering-PloUgh Research InstitUte Kenilworth, New Jersey 1998-2003 PhD, GradUation date: August, 2003 Mentor, Ben M. DUnn, PhD Department of Biochemistry and MolecUlar Biology University of Florida, Gainesville, FL 1995-1998 Master of Science, MolecUlar Biology/Biotechnology Mentor: Cindy PUtnam-Evans, PhD East carolina University, Greenville, Nc Nathan E. Goldfarb 2 1990-1994 Bachelor of Arts and Sciences, Biology Department of Biology Virginia Polytechnic InstitUte and State University BlacksbUrg, VA Industry Experience 2005-2007 Senior Scientist, Chemical Development Schering-PloUgh Research InstitUte Union, New Jersey 2007-2008 Chemist Vistakon corporation (Johnson & Johnson) Jacksonville, FL Teaching 2018-present Assistant Professor, chemistry Utah Valley University Orem, UT CHEM 3600, Biological Chemistry CHEM 3605, Biochemistry Lab CHEM 1225, Chemistry II Lab CHEM 1120, Elementary Organic Biochemistry CHEM 482R CHEM 489R 2015-2017 Assistant Professor, Biochemistry Department of PharmaceUtics and Biomedical Sciences California Health Sciences University Clovis, CA 93612 PHR 511 Biochemistry (CoUrse Director and InstrUctor) (for P1 PharmD stUdents; 30 TBL sessions) Team-based learning pedagogy 2012-2013 Mentor to Folasade OlajUyibe, PhD Visiting Professor, Nigeria Department of Biochemistry and MolecUlar Biology, University of Florida 2011-2012 Mentor to Wajahat Mahmood, doctoral stUdent Charles Darwin University/ Department of Biochemistry and MolecUlar Biology, University of Florida 2010-2011 Mentor to Sirilak Namwong, PhD Visiting LectUrer, Thailand Department of Biochemistry and MolecUlar Biology, University of Florida 2008-2015 Mentor to UndergradUate stUdents: Sara YU, Meray Ohanessian, Denis chen, Jose Garcia, David MarcUs, Ariana cooke, Jeyko GarUz, Rachel Leeman, Stephanie Sanchez, Krina Patel, Edith Brach-Sanchez Nathan E. Goldfarb 3 2008-2015 Mentor to lab techs: Jeyko GarUz, Daniil Shabashvilli, Tammy Flagg, Yang Tang Service 2019 Chair, FacUlty Search Committee, Utah Valley University 2018 National Science FoUndation Scholarship committee Member, Utah Valley University 2016-present FacUlty Advisor for StUdent National PharmaceUtical Association (SNPhA) California Health Sciences University Chapter 2016-present Enterprise Risk Management Subcommittee California Health Sciences University 2015-present FacUlty coUncil Committee, Research and Scholarship committee, Admissions and Progression committee, Honor coUncil committee, Departmental chair Search committee (chairman) 2015-present FacUlty Advisor for 8 gradUate stUdents California Health Sciences University 2015-present FoUnded the california Health Sciences University OUtdoor AdventUre clUb, FacUlty Advisor Volunteering 2017-present California State University, Fresno Department of chemistry Dr. cory Brooks’ Laboratory TubercUlosis DrUg Discovery Research. Goldfarb, N.E., Principal Investigator. Mentor to one Master’s degree stUdent: Ms. Shuchi Kakkad Publications 1. Abell A., Perez BL., Njkin S., Parish T., Dunn BM., and Goldfarb NE. Discovery of a potent, reversible, small molecUle inhibitor of Hip1, a novel tUbercUlosis drUg target. (manUscript in preparation). 2. Ostrov DA., DUnn BM., and Goldfarb NE. High resolUtion strUctUre of tUbercUlosis drUg target, Hip1, in its anhydroform. (manUscript in preparation). 3. Naffin-Olivos J., Daab, A., While A., Goldfarb NE, Rengarajan J. , Milne, A., LiU D., Baikovitz J., DUnn B.M., Rengarajan J., Petsko G.A., and Ringe D. crystal StrUctUre of Hip1, a novel serine protease drUg target for TubercUlosis. Biochemistry. 2017. 56 (19) 2435-2528. Front Cover of Journal. 4. LiU Z., HUang X., HU L., Pham L., Poole KM., Tang Y., Mahon B., Tang W., KUnhUa L., Goldfarb N.E., Dunn BM, McKenna R., FanUcci GE. Effects of Hinge Region NatUral Polymorphisms on HUman ImmUnodeficiency VirUs-1 Protease StrUctUre, Dynamics and DrUg-PressUre EvolUtion. Journal of Biological Chemistry. 2016. Nathan E. Goldfarb 4 5. Quiliano M., Mendoza A., Fong KY., Pabon A., Goldfarb NE., Fabing I., Vettorazii A., Lopez de cerian A., DUnn BM., Garavito G., Wright DW., Deharo E., Perez-Silanes S., Aldana I., Galiano S. Exploring the scope of new arylamino alcohol derivatives: Synthesis, antimalarial evalUation, toxicological stUdies, and target exploration. Int J Parasito DrUgs DrUg Resist. 2016. 6. 184-198. 6. Mahindra A., Gangwal R., Bansal S, Goldfarb N.E., DUnn B.M., Sangamwar A.T., and Jain R. Antiplasmodial activity of short peptide-based compoUnds. RSC Advances. 2015. 5, 22674-22684. 7. Sing A.K., Rathore S., Tang Y., Goldfarb N., DUnn B.M., Rajendran V., Ghosh P.c., Singh N., Latha N., Singh B.K., Rawat M., and Rathi B. Hydroxyethylamine based pthalimides as a new class of plasmepsin inhibitors: design, synthesis, and antimalarial evaluation. PLOS ONE. 2015. 10. 1-20. 8. Goldfarb N.E., Georgieva M., Naffin-Olivos J., Madan-Lala R., Dong L., Bizzell E., Valinetz E., Brandt G.S., YU S., Shabashvili D.E., Ringe D., DUnn B.M., Petsko G.A, and Rengarajan J. Hip1, a novel serine protease drug target for TUbercUlosis. PLOS Pathogens. 2014. 10(5):e1004132. 9. Goldfarb, N.E., Ohanessian, M., Garcia, P., Ostrov, D., and DUnn, B. Defective Hydrophobic Sliding Mechanism and Active Site Expansion in HIV-1 Protease DrUg Resistant Variant Gly48Thr/LeU89Met:Mechanisms for the Loss of SaqUinavir Binding Potency. Biochemistry. 2015. 54:2, 422-33. 10. Jones S.A., Neilsen P.M., Siew L, callen D.F., Goldfarb N.E., DUnn B.M., and Abell A.D. A Template- Based Approach to Inhibitors of calpain 2, 20S Proteasome, and HIV-1 Protease. ChemMedChem. 2013. doi: 10.1002/cmdc.201300387. 11. Goldfarb N.E. and DUnn B.M. HUman immUnodeficiency virUs 2 retropepsin. Handbook of Proteolytic Enzymes. 3 ed. vol 1. Rawlings, N.D., Salvesen, G. Elsevier Science Dewey. 2012. 199-203. 12. Goldfarb N.E. and DUnn B.M. HUman immUnodeficiency virUs 1 retropepsin. Handbook of Proteolytic Enzymes. 3 ed. vol 1. Rawlings, N.D., Salvesen, G. Elsevier Science Dewey. 2012. 1. 190- 198. 13. Li M., GUstchina A, Matúz K., Tözsér J., Namwong S., DUnn B., Goldfarb N.E., Wlodawer A. StrUctUral and biochemical characterization of the inhibitor complexes of XMRV protease. FEBS. 2011. 22:4413-24. 14. Goldfarb N.E., Lam M.T., Bose A.K., Patel A.P., and DUnn B.M. Electrostatic switches that mediate the pH-dependent conformational change of “short” recombinant hUman pseUdocathepsin D. Biochemistry. 2005. 44:48, 15725-33. 15. Beyer B.B., Goldfarb N.E., and DUnn B.M. Expression, pUrification, and characterization of aspartic endopeptidases: Plasmodium plasmepsins and “short” recombinant hUman pseUdocathepsin D. Current Protocols in Protein Science. 2003. 16. Wlowdawer A., Li M., GUstchina A., Zbigniew D., Uchida K., Oyama H., Oda K., Goldfarb N.E., and Dunn B.M. Inhibitor complexes of the Pseudomonas serine-carboxyl proteinase. Biochemistry. 2001, 40:51, 15602-15611. Nathan E. Goldfarb 5 17. Goldfarb N.E., Knoepfle N., and PUtnam-Evans c. constrUction of a psbC deletion strain in Synechocystis 6803. SAAS Bull. Biochem. Biotechnol. 1997, 10, 1-6. Abstracts 1. Nathan Goldfarb, Sarah YU, Daniil Shabashvilli, Maria Georgieva, Ranjna Madan-Lala, JacqUeline Naffin-Olivos, Gregory Petsko, Jyothi Rengarajan, and Ben M. DUnn. (2013) Hip1, a novel serine protease drug target for Tuberculosis. American Proteolysis Society. Kona, Hawaii. 2. Nathan Goldfarb, Sarah YU, Daniil Shabashvilli, Maria Georgieva, Ranjna Madan-Lala, JacqUeline Naffin-Olivos, Gregory Petsko, Jyothi Rengarajan, and Ben M. DUnn. (2013) Hip1, a novel serine