Product datasheet Anti-NELFB Antibody Catalog Number: A12025-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-NELFB Antibody Gene Name NELFB Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E. coli-derived human COBRA1 recombinant protein (Position: M1-E252). Purification Immunogen affinity purified. Observed MW 66KD Dilution Ratios Western blot: 1:500-2000 Direct ELISA： 1:100-1000 Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information Cofactor of BRCA1, also known as COBRA1, is a human gene that encodes NELF-B. NELF-B is a subunit of negative elongation factor (NELF), which also includes NELF-A (WHSC2), either NELF-C or NELF-D (TH1L), and NELF-E (RDBP). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1) and SPT5 (SUPT5H), to cause transcriptional pausing of RNA polymerase II. COBRA1 was initially identified in a yeast two-hybrid screen using the BRCT1 domain of BRCA1 as bait. Reference Anti-NELFB Antibody被引用在0文献中。 暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-NELFB Antibody Catalog Number: A12025-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data Figure 1. Western blot analysis of COBRA1 using anti-COBRA1 antibody (A12025-1). Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. anti- COBRA1 antigen affinity purified polyclonal antibody (Catalog # A12025-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for COBRA1 at approximately 66KD. The expected band size for COBRA1 is at 66KD. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Powered by TCPDF (www.tcpdf.org).