US 20170081655A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2017/0081655 A1 Gundling (43) Pub. Date: Mar. 23, 2017 (54) PURIFICATION OF NUCLEIC ACIDS USING Publication Classification COPPER-TITANIUM OXDES (51) Int. Cl. (71) Applicant: ABBOTT MOLECULAR INC. Des CI2N IS/TO (2006.01) Plaines, IL (US) (52) U.S. Cl. CPC ................................ CI2N 15/1006 (2013.01) (72) Inventor: Gerard J. Gundling, Des Plaines, IL (US) (21) Appl. No.: 15/209,318 (57) ABSTRACT (22) Filed: Jul. 13, 2016 O O The present disclosure relates to systems and methods for Related U.S. Application Data purifying nucleic acid. In particular, the present disclosure (60) Provisional application No. 62/192,444, filed on Jul. relates to systems and methods for purifying nucleic acids 14, 2015. using metal or metal oxide compositions. Patent Application Publication Mar. 23, 2017. 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Sheet 21 of 76 US 2017/0081655 A1 FIG 11A 1509 16:08 1707' 1806 1905 Al Pairs mMCumMT Tukey-Kramer 0.05 F.G. 11B 15:09 16:08 17:07 18:06 mMCumMTi Patent Application Publication Mar. 23, 2017. Sheet 22 of 76 US 2017/0081655 A1 FIG. 11C 21.5 - 0 A 21 (5 . "" O T - i.e. - 20.5 wom L 2O a 1311 1410 1509 1608 17:07' 1806 1905 AH Pairs nMCumMT Tukey-Kramer O.05 FIG. 11D 15:09 16:08 17:07 18:06 mMCumMTi Patent Application Publication Mar. 23, 2017. Sheet 23 of 76 US 2017/0081655 A1 F.G. 12A Abbott MultiAnalyze Wersion 5.01 alpha Median SD 2 3 4 5 6 7 8 9 O 112 Display Dye FAM 0.0027 O. : Ref. Dye ROX w C C.O. Current File: 082714MMP PCR 8272O141 01943 MC.csv ; 2 Setup File: newHCVunlocked mat G Plot Baselined y 100% 8 Well 62 F 2 C 25.33 MR 0.306 FCN 28.32 CNA 30.03 o Width 3.11 Final 6.31 Plot Display Rs. log Y Cycle Zoom FIG. 12B Abbott MultiAnalyze Version 5.01 alpha Median SD 1 2 3 4 5 as 7 8 10 1112 Display Dye vic 0.001) (): ; C O Ref. 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