LEED Certified Platinum Green Building Praj Industries and Praj Matrix Partnering for Bespoke Technology Development, Scale -up and Commercialization Pramod Kumbhar CTO © Praj Industries Ltd www.praj.net Corporate Overview Praj Matrix-R&D center enfinity : Praj Ligno-cellulosic (LC) Biorefinery Beyond Business 2 Corporate Overview Established in 1983 by first generation technopreneur, Pramod Chaudhari (Executive Chairman) Praj is a unique technology + engineering company, with Global leadership in ethanol arena End-to-end solutions Provider – concept to commissioning; feedstock handling to effluent treatment Offering TEMP-C1 solutions and products Praj Matrix: World Class R&D center Listed on NSE & BSE in India Strong Balance Sheet TEMP-C1 – Technology, Engineering, Manufacturing, Procurement, Construction 3 Vision, Mission & Values Vision We aspire to be the most preferred organization for all stakeholders through environment friendly, sustainable solutions that can make the world a better place Mission Develop and deliver cost- Invest and partner in effective, safe, clean and development and Be a socially reliable solutions that will realization of new ideas responsible Create wealth for maximize prosperity of recognizing the risks and corporate citizen our Stakeholders our customers working to mitigate them Values Integrity Innovation Passion Responsibility Agility Reliability 4 More than Three Decades of Building Trust 2008-14 2015 onwards . Moves 2G bioethanol technology to pilot scale . Launches Ethanol Plant Modernization . Enters high purity water segment through acquisition of Business Neela Systems (now called PRAJ HiPurity Systems) . Introduces ‘Enfinity’ 2G ethanol technology . Designs and launches new corporate identity . Commercializes 2G Demonstration Plant . Won 2G orders from Leading OMC’s 1991-98 . IPO in 1994 . Forays into synergistic fields like brewery . Enters South - East Asian market . Alliance with Filtrox for Filtration systems 1999-2007 . Enters South American, European & African markets . Established 100% market share in Colombia . Launch of new R&D setup (Matrix) 1983-90 . Commences development of 2G bioethanol technology . Inception: 1983 . Forays into process equipment, water & waste water treatment . Establishes In-house R&D Center . Venture funding by ICICI Globally recognised for proprietary technologies & engineering solutions for bio- fuels , bio-chemicals & water treatment solutions Facts at a glance 5 25% Revenue Manufacturing Business from Repeat $ 150 Mn + Facilities Customers > 50 % Over 750 1200+ Share in References Employees Emerging markets 90+ Research >30+ Years > 350 references Scientists Glorious Years Since from overseas In R&D center Inception Strong IP Presence across 22 Years Granted patents 17 5 Continents Consistent Dividend Applications 260 Paying Company 6 Global references… Russia Canada UK Germany Belgium Kazakhstan Hungary Romania Uzbekistan Turkey USA Spain Japan Nepal Egypt Dominican Republic Thailand Mexico Nigeria Barbados Vietnam Philippines Guatemala Myanamar Nicaragua Guyana Sierra Leone Uganda Ethiopia Colombia Kenya Srilanka Indonesia Peru Malawi Bolivia H.O. Mozambique Pune, India Swaziland Australia S. Africa Argentina 750 references , 35 countries, 5 continents 7 * Representative Business offerings Bioenergy High Purity Engineering Businesses Praj-Matrix-R&D center Solution Provider in Ethanol plants upto High purity Critical process Customized ZLD* applications for equipment & Effluent Research Services & Modernization & pharma, Brewery systems, Recycling & Solutions upgradation, biotech, plants Modular Process ZLD cosmetics Bio-Product 24X7 Customer Care, Skids After Sales Service, industry Division Renewable Natural Gas (RNG) *ZLD: Zero Liquid Discharge 8 Praj’s Global Ethanol Leadership Pharma Industrial/ Fuel Ethanol Grade Ethanol Perfumery Grade/ Others ethanol Praj : A one–stop-shop for ethanol plants, from variety of feedstock, grades of product and range of plant capacities. 9 Praj Matrix – R&D center R&D Centre of Praj Industries Ltd. 80,000 sq ft of Labs, Pilot Plants, and Offices 95 technologists . 20 PhDs, 65 Masters 5 Technology COEs . Biology, Chemistry, Engineering 16 Well Equipped Labs ISO-9001-2008 Analytical Labs Pilot Plants . 1 tpd Cellulosic Ethanol pilot plant . Multipurpose Pilot Plant Recognized research center by DSIR, Govt of India Bench and Pilot scale facilities enable validation of scientific assumptions and rapid commercialization 10 Centre of Excellence Ph.D.: 08 Post-graduates:23 Ph.D.: 02 Post-graduates:15 Biologists Chemists Engineers Ph.D.: 06 Post-graduates:06 Ph.D.: 01 Post-graduates:01 Total strength : 95 Ph.D.: 02 Post-graduates:18 We focus on Renewable Feed stocks Sustainability & Profitability Green Products Conversion Technologies © Praj Industries Ltd www.praj.net 12 Feedstock Expertise • Molasses Sugar cane molasses Over 7000 samples across globe Sugar beet molasses • Grain feed stocks Corn, Sorghum and Millets Wheat, Barley Rye Triticale and Oats Compositional database of over 2000 samples from different countries Compositions of wet grains, solubles and DDGS along with variances • Second Generation Feedstocks Sugar Cane residues Corn Residues Straws- Wheat, Rice, Cotton Palm residues and grasses. • Oil based: Fish oil Palm oil residues, Soy oil residues etc 13 Biochemicals Development at Praj Renewable Conversion Downstream Feedstock Technologies Processing Bio-chemical • Distillation • Fungal • Yeast • Extraction Products • Bacteria • Crystallization Chemo-catalytic • Membrane separations • Homogeneous • Chromatographic catalysis separations • Heterogeneous catalysis © Praj Industries Ltd www.praj.net 14 Current product/technology portfolio Fuel • Ethanol (2 G) • Isobutanol (with GEVO) Biochemicals • Furfural * • Xylitol • Ethyl levulinate • 2,3 BDO • Hydroxymethylfurfral (HMF)** Health and Nutrition • Phytosterol/Tocopherol • Tocotrienol • EPA-DHA • Hyaluronic acid • Natural antimicrobial peptides • * : IP licensed • ** : Under© Praj development Industries Ltd from LC sugars www.praj.net 15 Biochemicals Development at Praj Technology Capability Product Price Product Opportunities 1000 Hyaluronic Acid Molecular & Micro-Biology 300 Antimicrobial peptides 100 50 EPA+DHA Process Development Phytosterols - Vit E 10 $/kg Downstream Process Xylitol 2 Furfural Chemical Sciences Levulinic Acid 2-3 BDO Product Volume (TPY) © Praj Industries Ltd www.praj.net 16 Micro and molecular Biology Expertise Classical Mutagenesis Directed Evolution Genome Shuffling Microbiology Medium throughput microbial Screening Screening in deep well plates, shake flasks, 1 L fermenters Yeast cross-breeding Bioassay Development Cloning & Expression in Bacteria and Yeast Gene Deletions Molecular Biology Cofactor engineering Heterologous expression Protein secretion Targeted improvements for substrate utilization, by product reduction / elimination Enzyme characterization Enzyme purification and formulation Enzymology Enzyme performance evaluation Application Development Enzyme activity assay development Enzyme kinetic studies Yeast Development at Praj Matrix Molecular Yeast Yeast Breeding Typing Superior diploid parent Inferior diploid parent Reverse Engineering sporulation sporulation Superior hybrid diploid yeast sporulation screening screening Superior haploid Inferior haploid Crossing x Haploid segregants Superior hybrid diploid yeast screening Bioprospecting Metabolic Engineering Superior haploids Pooled whole genome sequencing Bioinformatic analysis for identification of Single Nucleotide Polymorphisms (SNP) Engineering of SNP in industrial yeast strain © Praj Industries Ltd www.praj.net 18 High Throughput Screening • Microbial Diversity Screening project Grant : Department of Biotechnology (DBT) Screening of 20,000 microbes from DBT culture bank 4 Target molecules of industrial relevance 320,000 assays Praj gets first right to commercialize © Praj Industries Ltd www.praj.net 19 enfinity-Praj’s LC Biorefinery © Praj Industries Ltd CONFIDENTIAL www.praj.net enfinity : Technology development Roadmap • Inauguration of Praj’s own and India’s first 2nd gen bio-refinery • Commercial offering • 12 TPD 2nd Gen Bio refinery Operational 2017 2014-15 Commercial demo plant engineering Pilot plant operation 2010-13 2008-10 Pilot Scale & Engineering 21 Value Chain Feedstock Enzymatic Distillation & Waste Water Feedstock Handling Pretreatment Fermentation Preparation System Hydrolysis Dehydration Treatment End to End integrated process Leveraging expertise in Process optimization & Integration © Praj Industries Ltd CONFIDENTIAL www.praj.net 22 Key Highlights of Praj’s Integrated Demo Plant • Integrated Bio refinery Demonstration Plant : Capacity – 1 MLPA Ethanol production • Multi feedstock processing capability: Rice (paddy) Straw, Cotton stalk, Corn Cobs & Corn Stover, Sugar Cane bagasse & Cane trash, Wheat Straw etc. • Multi-product - Designed to produce Bio-ethanol, Bio-gas / Bio-CNG, Bio fertilizer and provision for Production of Bio chemicals • End to End Technology demonstration from feedstock processing till end product and waste water treatment • Zero Process Liquid Discharge • Process Integration for optimization of Energy & Water consumption • GHG reductions of 93 % : Validated by European Petroleum company Validated by team of experts from Oil Marketing Companies Praj’s Ligno-Cellulosic Bio-refinery Mechanical and Hydrothermal Enzymatic hydrolysis pretreatment Lignocellulosic biomass Fermentation C5 sugars