G-Biosciences Protein Cross-Linkers Handbook & Selection Guide c G-Biosciences • 1-800-628-7730 • www.GBiosciences.com • Protein Estimation Assays • Lysis Buffers & Systems • Apoptosis Assays • Protein Fractionation Kits • Cytotoxicity Assays • Dialysis (Micro) System • SAM Methyltransferase Assays • Electrophoresis Clean-Up • Protease Assays • Concentration Systems • Phosphatase Assays • Contamination Removal • Peroxide Assay • Protease Inhibitor Cocktails • Proteomic Grade Detergents • Individual Protease Inhibitors • Research Grade Detergents • Protease Assays • Non-Ionic, Ionic & Zwitterionic • Proteases for Mass Spec. • Detergent Estimations • Sequencing Grade Proteases • Detergent Removal Systems • 1-Hour Western System • Gel Preparation Chemicals • Transfer Buffers & Membranes • Protein Marker Ladders • Membrane Stains • Electrophoresis Buffers • Blocking Buffers • Reducing & Alkylating Reagents • Secondary Antibodies • Protein Gel Stains • Detection Reagents • Reprobing Reagents • Protein Sample Preparation • Affinity Resins • Protein Clean-Up Systems • 6X His Protein Purification Kits • Electrophoresis Reagents • GST Protein Purification Kits • Mass Spec Grade Protease • Antibody Purification • InGel Digestion Kits • Activated Resins • Peptide Generation Reagents • Buffers & Reagents • Biotin Labeling • Carrier Proteins • Cell Surface Protein Labeling • Peptide Coupling Systems • Agarose Coupling Kits • Antibody Purification Resins • Fluorescent Dye Labeling Kits • Antibody Fragmentation Kits • Enzyme Labeling Systems • Coated Plates • Blocking Buffers • Homobifunctional • Wash Buffers • Heterobifunctional • Secondary Antibodies • Optimizer Systems • Detection Reagents • Cross-Linking Systems • Antibody Labeling Systems • Apoptosis Assays • DNA Isolation • Cytotoxicity Assays • Transformation & Screening • SAM Methyltransferase Assays • Polymerase Chain Reaction • Protease Assays • Agarose Electrophoresis • Phosphatase Assays • RNA Isolation • Peroxide Assay • Yeast Transformation • ELISA Table Of Contents Introduction 2 Protein Reduction & Modification 13 ™ Cross-Linking Applications ..............................................2 Protein-S-S-Reductant .......................................................................13 Structural & Functional Studies ........................................................2 Immobilized Reductant ......................................................................13 Protein & Receptor Interactions .......................................................2 TCEP ...........................................................................................................13 For Immunological Tools ....................................................................2 Dithiothreitol (DTT) ..............................................................................13 Cell Membrane Structural Studies ..................................................2 ß-Mercaptoethanol ..............................................................................14 Cell Surface Studies ..............................................................................2 Ellman’s Reagent ...................................................................................14 Solid-Phase Immobilization ..............................................................2 Reaction Accessories ............................................................................14 ™ Preparation of Immunotoxins...........................................................2 Optimizer Buffer ...................................................................................14 Protein-Protein Conjugation .............................................................2 Protein to DNA/RNA Cross-Linking .................................................2 Iodination Reagents 15 Reactive Group Transfer ......................................................................2 Bolton-Hunter Reagent (SHPP) ........................................................15 Iodination Reagent ...............................................................................15 Cross-Linking 3 Sulfo SHPP ................................................................................................15 Primary Amine Reactive ..................................................4 Amino Acid Side Chain Modifiers 16 Sulfhydryl Reactive ..........................................................5 4-Vinylpyridine .......................................................................................16 Carbohydrate Reactive ....................................................6 Citroconic Anhydride ...........................................................................16 Carboxyl Reactive .............................................................6 L-Cysteine·HCL, monohydrate ..........................................................16 p-Hydroxyphenyl Glyoxal ...................................................................16 Photoreactive ....................................................................7 SATA ...........................................................................................................17 Sodium Metaperiodate .......................................................................17 Selection Guide 8 Sulfo NHS .................................................................................................17 Homobifunctional Cross Linkers .....................................8 Sulfo NHS Acetate .................................................................................17 BSOCOES ..................................................................................................8 TNBS ...........................................................................................................17 DPDPB .......................................................................................................8 Traut’s Reagent .......................................................................................17 DSS .............................................................................................................8 DST .............................................................................................................8 Accessories 18 Sulfo DST ..................................................................................................8 Tube-O-Reactor™ ....................................................................................18 DSP .............................................................................................................8 Solvents ....................................................................................................18 DTSSP .........................................................................................................8 EGS ..............................................................................................................8 Sample Preparation Accessories 19 Heterobifunctional Cross Linkers ....................................9 Dialysis Systems ................................................................18 MBS .............................................................................................................9 Tube-O-DIALYZER™ ................................................................................18 Sulfo MBS .................................................................................................9 DIALYZER-Enhance™ .............................................................................20 GMBS .........................................................................................................9 Tube-O-DIALYZER™ Accessories .......................................20 Sulfo GMBS ..............................................................................................9 Tube-O-Array™ ........................................................................................20 EMCH .........................................................................................................9 Centrifuge Tube-Adapter ...................................................................20 EMCS ..........................................................................................................9 Tube-O-Tanks .........................................................................................20 Sulfo EMCS ...............................................................................................9 Micro Dialysis Cups ...............................................................................20 PMPI ...........................................................................................................9 Stirring Balls ............................................................................................20 SIAB ............................................................................................................10 Floats ..........................................................................................................20 SMCC ..........................................................................................................10 SMPB ..........................................................................................................10 Desalting & Buffer Exchange ...........................................21 ™ Sulfo SIAB .................................................................................................10 Spin-OUT ................................................................................................21 ™ Sulfo SMCC ..............................................................................................10