Methods in Molecular Biology 1735 Paul C. Guest Editor Investigations of Early Nutrition E ects on Long- Term Health Methods and Applications M ETHODS IN M OLECULAR B IOLOGY Series Editor John M. Walker School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Investigations of Early Nutrition Effects on Long-Term Health Methods and Applications Edited by Paul C. Guest Debden, Essex, UK Editor Paul C. Guest Debden, Essex, UK ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7613-3 ISBN 978-1-4939-7614-0 (eBook) https://doi.org/10.1007/978-1-4939-7614-0 Library of Congress Control Number: 2017964007 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A. Preface Environmental factors such as suboptimal parental diet can lead to perturbations of in utero growth and development. In turn, this can have long-lasting negative effects on health in later life by leading to increased risk and earlier onset of diseases such as type 2 diabetes, obesity, cardiovascular conditions, cancer, and neurological disorders. In addition, future generations are at risk of inheriting these conditions through nongenetic mechanisms. Elucidating the molecular pathways involved in these processes may help in the design of prevention strategies and may also lead to the identification of biomarkers and potential drug targets for improved patient management. This book contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcrip- tomics, and metabolomics which aim to address this dilemma as well as help to pinpoint new treatment strategies. It also describes the generation of several models in the area and methods for assessing health as well as potential approaches for reversing or minimizing disease effects. The chapter contributions come from authors representing five out of the seven continents, including countries such as the UK, Germany, Switzerland, Italy, the USA, Mexico, Brazil, Chile, Australia, New Zealand, and Japan. This reflects the growing impor- tance of this topic throughout the world. The book will be of high interest to researchers, clinical scientists, physicians, as well as the major drug companies as it gives insights into the latest ideas and technologies enabling progress in this area. It will also be of high interest to both technical and bench scientists as it gives step-by-step instructions on how to carry out each of the protocols. Lastly, it will provide important information on disease mechanisms, as each method will be described in the context of specific disease or therapeutic areas. Debden, Essex, UK Paul C. Guest v Contents Preface . ................................................................... v Contributors................................................................. xi PART IREVIEWS 1 Nutritional Programming Effects on Development of Metabolic Disorders in Later Life................................................... 3 Thomas Prates Ong and Paul C. Guest 2 Effects of Prenatal Nutrition and the Role of the Placenta in Health and Disease ................................................... 19 Leslie Myatt and Kent L. Thornburg 3 Developmental Origins of Stress and Psychiatric Disorders ................... 47 Francesca L. Guest and Paul C. Guest 4 Proteomic Studies of Psychiatric Disorders . .............................. 59 Paul C. Guest 5 Developmental Origins of Breast Cancer: A Paternal Perspective.............. 91 Camile Castilho Fontelles, Raquel Santana da Cruz, Leena Hilakivi-Clarke, Sonia de Assis, and Thomas Prates Ong 6 Point-of-Care Testing and Personalized Medicine for Metabolic Disorders . ............................................................ 105 Francesca L. Guest and Paul C. Guest 7 Pregnancy and Lactation: A Window of Opportunity to Improve Individual Health ............................................ 115 Guadalupe L. Rodrı´guez-Gonza´lez, Diana C. Castro-Rodrı´guez, and Elena Zambrano 8 Utility of Small Animal Models of Developmental Programming ............. 145 Clare M. Reynolds and Mark H. Vickers PART II PROTOCOLS 9 Generation of Maternal Obesity Models in Studies of Developmental Programming in Rodents . ............................................. 167 Paul D. Taylor, Phillippa A. Matthews, Imran Y. Khan, Douglas Rees, Nozomi Itani, and Lucilla Poston 10 Generation of the Maternal Low-Protein Rat Model for Studies of Metabolic Disorders .................................................. 201 Dan Ma, Susan E. Ozanne, and Paul C. Guest 11 Investigation of Paternal Programming of Breast Cancer Risk in Female Offspring in Rodent Models .................................... 207 Camile Castilho Fontelles, Raquel Santana da Cruz, Leena Hilakivi-Clarke, Sonia de Assis, and Thomas Prates Ong vii viii Contents 12 Studies of Isolated Peripheral Blood Cells as a Model of Immune Dysfunction ................................................. 221 Hassan Rahmoune and Paul C. Guest 13 Studies of a Neuronal Cell Line as a Model of Psychiatric Disorders . ............................................................ 231 Keiko Iwata 14 Assessment of Placental Transport Function in Studies of Disease Programming................................................. 239 Amanda N. Sferruzzi-Perri 15 Assessment of Fatty Liver in Models of Disease Programming . ............... 251 Kimberley D. Bruce and Karen R. Jonscher 16 Capillary Blood Sampling from the Finger . .............................. 267 Kieron Rooney 17 Physical Activity Assessment in Clinical Studies of Substance Use Disorder ........................................................... 273 Rhiannon Dowla, Bridin Murnion, Kia Roberts, Jonathan Freeston, and Kieron Rooney 18 Cardiopulmonary Exercise Testing . ..................................... 285 Derek Tran 19 Cardiovascular Assessment in Human Research............................. 297 Marjan Mosalman Haghighi and Julian Ayer 20 Nutritional Programming Effects on the Immune System.................... 311 Donald B. Palmer 21 Small RNA Sequencing: A Technique for miRNA Profiling . ............... 321 Lucas Carminatti Pantalea˜o and Susan E. Ozanne 22 Pulse-Chase Biosynthetic Radiolabeling of Pancreatic Islets to Measure Beta Cell Function ........................................... 331 Paul C. Guest 23 Rapid and Easy Protocol for Quantification of Next-Generation Sequencing Libraries .................................................... 343 Steve F. C. Hawkins and Paul C. Guest 24 Telomere Length Analysis: A Tool for Dissecting Aging Mechanisms in Developmental Programming .......................................... 351 Jane L. Tarry-Adkins and Susan E. Ozanne 25 Qualitative and Quantitative NMR Approaches in Blood Serum Lipidomics . .................................................... 365 Banny Silva Barbosa, Lucas Gelain Martins, Ta´ssia B. B. C. Costa, Guilherme Cruz, and Ljubica Tasic 26 In Vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models ............................ 381 Kaio F. Vitzel, Marco A. Fortes, Gabriel Nasri Marzuca-Nassr, Maria V.M. Scervino, Carlos H. Pinheiro, Leonardo R. Silveira, and Rui Curi Contents ix 27 Experimental Model of Skeletal Muscle Laceration in Rats ................... 397 Phablo Abreu, Gabriel Nasri Marzuca-Nassr, Sandro Massao Hirabara, and Rui Curi 28 Neuropsychiatric Sequelae of Early Nutritional Modifications: A Beginner’s Guide to Behavioral Analysis . .............................. 403 Ann-Katrin Kraeuter, Paul C. Guest, and Zolta´n Sarnyai 29 Hyperlocomotion Test for Assessing Behavioral Disorders ................... 421 Dan Ma and Paul C. Guest 30 2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation ................................................... 427 Paul C. Guest 31 Mass Spectrometry Profiling of Pituitary Glands ............................ 439 Divya Krishnamurthy, Hassan Rahmoune, and Paul C. Guest 32 Multiplex Immunoassay Profiling