Fasciculin II from Dendroaspis angusticeps Product Number F4293 Storage Temperature −20 °C Synonyms: Toxin F7 Precautions and Disclaimer Cas number: 95506-56-2 This product is for laboratory research use only. Please consult the Material Safety Data Sheet for Sequence: information regarding hazards and safe handling TMCYSHTTTS RAILTNCGEN SCYRKSRRHP practices. PKMVLGRGCG CPPGDDNLEV KCCTSPDKCN Y Disulfide bonds: C3-C22, C17-C39, C41-C52, C53-C59 Preparation Instructions Fasciculin II is readily soluble in water. Product Description Fasciculin II is a neurotoxin isolated from the venom of Storage/Stability Dendroaspis angusticeps (eastern green mamba). It is Fasciculin II is very stable and should be stored as a 61 amino acid monomeric peptide with four disulfide supplied at −20 °C. bonds and a molecular weight of 6735 Da.1 The calculated pI is 8.31. References 1. Karlsson, E. et al., Fasciculins, anticholinesterase Fasciculin II is an inhibitor of acetylcholinesterase. It toxins from the venom of the green mamba acts by binding to the gorge entrance of Dendroaspis angusticeps. J. Physiol., 79, 232-240 acetylcholinesterase, sterically blocking ligand access (1984). and causing other conformational changes in the 2. Tai, K., et al., Mechanism of acetylcholinesterase enzyme including disruption of the catalytic site.2 The inhibition by fasciculin: a 5 ns molecular dynamics binding constants (Ki) for acetylcholinesterase in simulation. J. Am. Chem. Soc. 124, 6153-6161 human erythrocytes is 1.1 x 10-10 M (37 °C), in rat (2002). muscle it is 1.2 x 10-10 M (37 °C), and in the electric 3. Dajas-Bailador, F., et al., Effects of α-erabutoxin, organ of the eel Electrophorus electricus, it is α-bungarotoxin, α-cobratoxin and fasciculin on the 3 x 10-10 M (22 °C).1 Fasciculin II has also been nicotine-evoked release of dopamine in the rat shown to block nicotine-induced release of dopamine striatum in vivo. Neurochem. Int., 33, 307-312 at a concentration of 4.2 µM.3 In mice, it has a (1998). LD50 >20 µg/g (i.v.). DMG 11/02 Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. .