SANTA CRUZ BIOTECHNOLOGY, INC. PSA (A67-B/E3): sc-7316 The Power to Question BACKGROUND APPLICATIONS Prostate specific antigen (PSA), also designated gamma-seminoprotein, sem- PSA (A67-B/E3) is recommended for detection of prostate specific antigen inin, p30 antigen, semenogelase, and kallikrein 3 (KLK3), was first identified of human and rat origin by Western Blotting (starting dilution 1:200, dilu- as a glycoprotein in human seminal plasma. PSA was determined by sequ- tion range 1:100-1:1000), immunoprecipitation [1–2 µg per 100–500 µg of ence similarity to be a member of the kallikrein subfamily of trypsin prote- total protein (1 ml of cell lysate)], immunofluorescence and immunohisto- ases. PSA is a serine protease that hydrolyzes the major human seminal chemistry (including paraffin-embedded sections) (starting dilution 1:50, protein, the seminal plasma mobility inhibitor precursor, or semenogelin I dilution range 1:50-1:500). (SPMIP or SgI), which leads to semen liquification. PSA production and Suitable for use as control antibody for PSA siRNA (h): sc-29458. expression are highest in normal, benign hyperplastic and cancerous tissues of the prostate, although PSA has also been detected in accessory male sex Molecular Weight of PSA: 34 kDa. glands and in breast cancer. PSA has been identified as an aid in the early Positive Controls: LNCaP cell lysate: sc-2231, AT-3 whole cell lysate or detection of prostate cancer and is a commonly used tumor marker. COLO 320DM cell lysate: sc-2226. REFERENCES RECOMMENDED SECONDARY REAGENTS 1. Watt, K.W., et al. 1986. Human prostate-specific antigen: structural and To ensure optimal results, the following support (secondary) reagents are functional similarity with serine proteases. Proc. Natl. Acad. Sci. USA 83: recommended: 1) Western Blotting: use goat anti-mouse IgG-HRP: sc-2005 3166-3170. (dilution range: 1:2000-1:32,000) or Cruz Marker™ compatible goat anti- 2. Schaller, J., et al. 1987. Isolation, characterization and amino-acid mouse IgG-HRP: sc-2031 (dilution range: 1:2000-1:5000), Cruz Marker™ sequence of gamma-seminoprotein, a glycoprotein from human seminal Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: plasma. Eur. J. Biochem. 170: 111-120. sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/ 2.0 ml). 3. Lundwall, A., et al. 1987. Molecular cloning of human prostate specific 3) Immunofluorescence: use goat anti-mouse IgG-FITC: sc-2010 (dilution antigen cDNA. FEBS Letts. 214: 317-322. range: 1:100-1:400) or goat anti-mouse IgG-TR: sc-2781 (dilution range: 4. Catalona, W.J., et al. 1993. Detection of organ-confined prostate cancer is 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. 4) Immunohis- increased through prostate-specific antigen-based screening. JAMA 270: tochemistry: use ImmunoCruz™: sc-2050 or ABC: sc-2017 mouse IgG 948-954. Staining Systems. 5. Seregni, E., et al. 1996. Biochemical characteristics and recent biological knowledge on prostate-specific antigen. Tumori 82: 72-77. DATA 6. Robert, M., et al. 1997. Characterization of prostate-specific antigen pro- AB - teolytic activity on its major physiological substrate, the sperm motility 78 K inhibitor precursor/semenogelin I. Biochem. 36: 3811-3819. 50 K - SOURCE 34 K - PSA (A67-B/E3) is a mouse monoclonal antibody raised against amino acids <PSA 1-261 representing full length PSA p30 of human origin. 28 K - PSA (A67-B/E3): sc-7316. Western blot analysis of PSA (A67-B/E3): sc-7316. Immunoperoxidase staining PRODUCT PSA expression in AT-3 (A) and COLO 320DM (B) of formalin-fixed, paraffin-embedded human prostate whole cell lysates. tissue cytoplasmic staining. Each vial contains 200 µg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. SELECT PRODUCT CITATIONS STORAGE 1. Gupta, S., et al. 2002. Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of prostate carcinoma cells. Oncogene 21: 3727-3738. shipment. Non-hazardous. No MSDS required. 2. Kim, D., et al. 2002. Androgen receptor expression and cellular prolifera- RESEARCH USE tion during transition from androgen-dependent to recurrent growth after For research use only, not for use in diagnostic procedures. castration in the CWR22 prostate cancer xenograft. Am. J. Pathol. 160: 219-226. PROTOCOLS 3. Le, H.T., et al. 2003. Plant-derived 3,3'-Diindolylmethane is a strong See our web site at www.scbt.com or our catalog for detailed protocols and androgen antagonist in human prostate cancer cells. J. Biol. Chem. 278: support products. 21136. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.