54th Annual Drosophila Research Conference 2013 Drosophila Genetics Washington, DC, USA 3-7 April 2013 ISBN: 978-1-62748-965-2 Printed from e-media with permission by: Curran Associates, Inc. 57 Morehouse Lane Red Hook, NY 12571 Some format issues inherent in the e-media version may also appear in this print version. Copyright© (2013) by the Genetics Society of America (GSA) All rights reserved. Printed by Curran Associates, Inc. (2013) For permission requests, please contact the Genetics Society of America (GSA) at the address below. Genetics Society of America (GSA) 9650 Rockville Pike Bethesda MD 20814-3998 Phone: (301) 634-7300 Fax: (301) 634-7079 [email protected] Additional copies of this publication are available from: Curran Associates, Inc. 57 Morehouse Lane Red Hook, NY 12571 USA Phone: 845-758-0400 Fax: 845-758-2634 Email: [email protected] Web: www.proceedings.com OPENING/GENERAL SESSIONS Wednesday, April 3 7:00 PM–9:00 PM Thursday, April 4 8:30 AM–12:35 PM Opening General Session Plenary Session I Co-Moderators: Richard Mann, Columbia University, New Moderator: David Stern, Janelia Farm Research York and Kristin Scott, University of California, Berkeley Campus, Ashburn, Virginia Room: Marriott Ballroom Salons 1-3, Lobby Level Room: Marriott Ballroom Salons 1-3, Lobby Level Presentations: 8:30 am Image Award Presentation. David Bilder. Presentations: University of California, Berkeley. 7:00 pm Welcome and Opening Remarks. Richard Mann. Columbia University, New York. 7:15 pm GSA Welcome and Update. Adam Fagen. Genetics Society of America, Bethesda, Maryland. 8:35 am Molecular Mechanisms of Axon 1 7:25 pm Larry Sandler Award Presentation. Ken Degeneration. Marc R. Freeman. Dept Neurobiology, Irvine. Rutgers University, Waksman Institute, Univ Massachusetts Med Sch/HHMI, Worcester, MA. Piscataway, New Jersey. 7:30 pm Larry Sandler Lecture. 9:05 am Genetic Approaches to Dissecting Neural 1 8:00 pm Jules Hoffmann Introduction. Kristin Scott. Computation in the Visual System. Tom Clandinin. University of California, Berkeley. Dept of Neurobiology, Stanford University, CA. 9:35 am The Genomics of Speciation and Pattern 1 Evolution in (butter)flies. Chris Jiggins. Dept 8:05 pm Innate Immunity : From Flies to 1 Zoology, University of Cambridge, Cambridge, Humans. Jules A. Hoffmann. IBMC, University of United Kingdom. Strasbourg, Strasbourg, France. 10:05 am – Break 8:50 pm Presentation of George W. Beadle Award to 10:30 am Creating Gradients by Morphogen 2 R. Scott Hawley, Stowers Institute for Medical Shuttling. Naama Barkai. Weizman Institute, Research. Adam Fagen. Genetics Society of America, Rehovot, Israel. Bethesda, MD 11:00 am Maintenance of Niche Function and 2 Tissue Homeostasis During Aging. Leanne Jones. Laboratory of Genetics, Salk Inst, La Jolla, CA. 11:30 am Histone Genetics in Drosophila 2 Müller. Max-Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Bavaria, Germany. 12:00 noon Presentation of FASEB Excellence in Science Award to Terry Orr-Weaver. Judith Bond. President, FASEB, Bethesda, MD. 12:05 pm Diamonds in the Rough: Finding Paradigms in Drosophila Developmental Strategies. Terry Orr-Weaver. Whitehead Institute, MIT, Cambridge Massachusetts. OPENING/GENERAL SESSIONS Sunday, April 7 8:30 AM–12:00 PM NOON Notes Plenary Session II Moderator: Hannele Ruohola-Baker, University of Washington, Seattle Room: Marriott Ballroom Salons 1-3, Lobby Level Presentations: 8:30 am Poster Awards. Hannele Ruohola-Baker. University of Washington, Seattle. 8:35 am Information, Enhancers, and Cell 2 Signaling: a View from the Binding Site. Scott Barolo. University of Michigan Medical School, Ann Arbor, MI. 9:05 am “The piRNA Pathway: a Small RNA- 3 Based Innate Immune System”. Greg Hannon. HHMI, Cold Spring Harbor Lab, One Bungtown Road, Cold Spring Harbor, NY. 9:35 am Transcription Factor Network Dynamics 3 in Development. Ilaria Rebay. Ben May Dept, Univ Chicago, Chicago, IL. 10:05 am – Break 10:30 am The Role of Nuclear Pore Proteins in 3 Developmental Gene Regulation. Martin W. Hetzer. Salk Inst for Biological Studies, La Jolla, CA. 11:00 am Stem Cells to Synapses: Regulation of 4 Self-Renewal and Differentiation in the Nervous System. Andrea H. Brand. The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom. 11:30 am Neurodegeneration and Aging: Insight 4 from Drosophila. Nancy M. Bonini. Dept Biol, 306 Leidy Labs, Univ Pennsylvania/HHMI, Philadelphia, PA.Moderator: Erika Matunis, Johns Hopkins University, Baltimore Concurrent Platform Sessions THURSDAY, APRIL 4 4:30-6:30 pm Program number is in bold above title. The first author is the presenter. Full abstracts can be found online Techniques and Functional Genomics/Systems Biology Co-Moderators: Tzumin Lee, Janelia Farms Research Institute, and Ward Cell Division and Growth Control Odenwald, NIH, and Thomas Gregor, Princeton University, and Richard Carthew, Northwestern University, Co-Moderators: Robert Duronio, University of North Carolina, Chapel Hill and Pat O'Farrell, University of California, San Francisco Room: Marriott Ballroom Salon 1, Lobby Level Room: Marriott Ballroom Salon 2, Lobby Level 1 - 4:30 A new frontier for the Duplication Consortium: retrofitted BACs that span 4 9 - 4:30 very large Drosophila genes and the 4th chromosome. Koen Venken1,2, Proper chromosome segregation and spindle assembly require both 7 Stacy Holtzman3, Soo Park4, Joe Carlson4, Roger Hoskins4, Hugo kinetochore and central spindle components in Drosophila Bellen1, Thom Kaufman3. 1) Molec & Human Genetics, BCM, Houston, oocytes. Sarah J. Radford, Kim S. McKim. Waksman Institute, TX; 2) Biochem & Molecular Biology, BCM, Houston, TX; 3) Biology, Rutgers University, Piscataway, NJ. IA, Bloomington, IN; 4) Life Sciences Division, LBNL, Berkeley, CA. 10 - 4:45 2 - 4:45 The oocyte-to-embryo transition requires APC/C mediated destruction 7 1 Captured segment exchange: A strategy for custom engineering large 5 of Matrimony, a POLO kinase inhibitor. Zachary J. Whitfield , 2 2 1 genomic regions in Drosophila melanogaster. Jack R. Bateman, Michael Jennifer Chisholm , R. Scott Hawley , Terry L. Orr-Weaver .1) F. Palopoli, Sarah T. Dale, Jennifer E. Stauffer, Anita L. Shah, Whitehead Institute for Biomedical Research, MIT, Cambridge, MA; 2) Justine E. Johnson, Conor W. Walsh, Hanna Flaten, Christine M. Stowers Institute for Medical Research, Kansas City, MO. Parsons. Biology Department, Bowdoin College, Brunswick, ME. 11 - 5:00 8 3 - 5:00 Regulation of the asymmetric centrosome maturation cycle in neural stem cells. Dorothy A. Lerit, Nasser M. Rusan. Cell Biology and Gene Targeting with TALENs in Drosophila. Dana Carroll1, Kelly J. 5 1 1 2 Physiology Center, NHLBI, National Institutes of Health, Bethesda, Beumer , Jonathan K. Trautman , Michelle Christian , Timothy J. MD. Dahlem3, Cathleen Lake4, R. Scott Hawley4, David J. Grunwald3, 2 Daniel F. Voytas . 1) Dept Biochem, Univ Utah Sch Med, Salt Lake City, 12 - 5:15 UT; 2) Dept Genetics, Cell Biology & Development, University of The role of corp in apoptosis following DNA damage. Riddhita 8 Minnesota, Minneapolis, MN; 3) Dept Human Genetics, Univ Utah Sch Chakraborty, Simon W. Titen, Kent G. Golic. Department of Med, Salt Lake City, UT; 4) Stowers Institute, Kansas City, MO. Biology, University of Utah, Salt Lake City, UT. 4 - 5:15 13 - 5:30 Pyrimidine salvaging enzyme UPRTase is active in Drosophila and limits 5 Tissue repair through cell competition and compensatory cellular 8 the specificity of tissue specific RNA isolation by 4TU tagging. Arpan hypertrophy in postmitotic epithelia. Yoichiro Tamori, Wu-Min Ghosh, MaryJane Shimmel, Emma Leof, Michael O'Connor. Gen Cell Deng. Biological Science, Florida State University, Tallahassee, FL. & Development, Univ Minnesota Twin Cities, Minneapolis, MN. 14 - 5:45 5 - 5:30 The transcriptional response to tumorigenic polarity loss. Brandon D. 9 Sequencing mRNA from cryo-sliced Drosophila embryos to determine 6 Bunker1, Anne-Kathrin Classen2, Tittu T. Nellimoottil3, David genome-wide spatial patterns of gene expression. Peter A. Combs1, Bilder1. 1) Molecular and Cell Biology, University of California- Michael B. Eisen2,3. 1) Biophysics Graduate Group, University of Berkeley, Berkeley, CA 94720; 2) Biology II, Ludwig-Maximilians- California, Berkeley, CA; 2) Department of Molecular and Cell Biology, University, Munich, D-82152 Germany; 3) Biological Sciences, University of California, Berkeley, CA; 3) Howard Hughes Medical University of Southern California, Los Angeles, CA 90033. Institute, University of California, Berkeley, CA. 15 - 6:00 6 - 5:45 Cell competition as a mechanism that can promote tumour growth 9 Mechanical aspects of fruit fly gastrulation. Konstantin Doubrovinski 6 through JNK activation. Luna L. Ballesteros-Arias, Verónica Bing He1, Oleg Polyakov1, Eric Wieschaus1,2. 1) Princeton University, Saavedra, Ginés Morata. Centro de Biología Molecular Severo Princeton, NJ; 2) Howard Hughes Medical Institute. Ochoa, Madrid, Spain. 7 - 6:00 16 - 6:15 Identification and verification of genes involved in apoptosis-induced 9 6 Genomic and transcriptomic analysis of diapause—an important life proliferation in Drosophila. Yun Fan1,2, Andreas Bergmann2. 1) history trait in Drosophila melanogaster. Xiaqing Zhao1, Alan 2 2 1 School of Biosciences, University of Birmingham, Birmingham, United Bergland , Dmitri Petrov , Paul Schmidt . 1) Dept. of Biology, Kingdom; 2) Cancer Biology, UMass Medical School, Worcester, University of Pennsylvania, Philadelphia,