苏州工业园区双圩路9号1幢 邮 编 ： 215000 电 话 ： 0512-88856768 SEC13 Antibody (Center) Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP10738c Specification SEC13 Antibody (Center) - Product info Application WB, FC Primary Accession P55735 Other Accession NP_899195.1 Reactivity Human Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Clone Names RB23825 Calculated MW 35541 SEC13 Antibody (Center) - Additional info Gene ID 6396 Other Names SEC13 Antibody (Center) (Cat. Protein SEC13 homolog, SEC13-like protein 1, SEC13-related #AP10738c) western blot analysis in protein, SEC13, D3S1231E, SEC13L1, SEC13R HepG2 cell line lysates (35ug/lane).This demonstrates the SEC13 antibody Target/Specificity detected the SEC13 protein (arrow). This SEC13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-100 amino acids from the Central region of human SEC13. Dilution WB~~1:1000 FC~~1:10~50 Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. Storage Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. SEC13 Antibody (Center) (Cat. #AP10738c) flow cytometric analysis of Precautions HepG2 cells (right histogram) compared SEC13 Antibody (Center) is for research use only and not for to a negative control cell (left use in diagnostic or therapeutic procedures. histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. SEC13 Antibody (Center) - Protein Information Name SEC13 Function Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles (PubMed:<a href="http://www.uniprot.org/citations/8972206" target="_blank">8972206</a>). Required for the exit of adipsin (CFD/ADN), an adipocyte-secreted protein from the endoplasmic reticulum (By similarity). Cellular Location Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Lysosome membrane. Note=In interphase, localizes at both sides of the NPC. SEC13 Antibody (Center) - Protocols Provided below are standard protocols that you may find useful for product applications. • Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture SEC13 Antibody (Center) - Background The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. SEC13 Antibody (Center) - References Nielsen, A.L. Biochem. Biophys. Res. Commun. 388(3):571-575(2009) Townley, A.K., et al. J. Cell. Sci. 121 (PT 18), 3025-3034 (2008) : Stagg, S.M., et al. Cell 134(3):474-484(2008) Hsia, K.C., et al. Cell 131(7):1313-1326(2007) Glavy, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(10):3811-3816(2007) SEC13 Antibody (Center) - Citations LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Powered by TCPDF (www.tcpdf.org).