Anti-SPOP monoclonal antibody, clone 0C8.2 (CABT-B1421) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Immunogen GST-tagged recombinant protein corresponding to human SPOP. Isotype IgG2a, κ Source/Host Mouse Species Reactivity Human Clone 0C8.2 Purification Protein G Purified Conjugate Unconjugated Applications WB, IHC Molecular Weight ~42 kDa observed. Uncharacterized band(s) may appear in some lysates. Format Liquid Concentration Please refer to lot specific datasheet. Size 100 µg Buffer 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Preservative 0.05% Sodium Azide Storage Stable for 1 year at 2-8°C from date of receipt. BACKGROUND Introduction Speckle-type pox virus and zinc finger (POZ) protein, also known as HIB homolog 1 or Roadkill homolog 1, is encoded by the SPOP gene (also known as TEF2 or BTBD32) in human (Entrez Gene ID 8405). SPOP protein is a component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex and mediates recruitment of target proteins containing the SPOP-binding consensus (SBC) motif. The 42 kDa SPOP protein comprises an N-terminal MATH domain, a bric-a-brac, tramtrack and broad complex (BTB)/POZ domain, a 3-box domain, 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved and a C-terminal nuclear localization sequence. The MATH domain is primarily involved in substrate recognition/binding. Known substrates with SBC motifs include Macro H2A, Puc (Puckered), Daxx (Death domain–associated protein), and Gli (Glioma-associated oncogene). Prostate and endometrial cancers are frequently identified with SPOP mutations in the meprin and TRAF (Tumor necrosis factor receptor-associated factor) homology (MATH) domain, which can potentially lead to altered substrate binding activities. The SPOP protein sequence is 100% conserved among human, murine, and rat (UniProt O43791, Q6ZWS8, and B2RYD9, respectively) species. GENE INFORMATION Entrez Gene ID 8405 UniProt ID O43791 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.