No Bees No Life APITHERAPY Symposium Lukovica and Maribor, Slovenia, rd th 23 -24 October 2015 BOOK OF ABSTRACTS 1 Apitherapy Symposium No bee no life Editor: Andreja Kandolf Borovšak, Nataša Lilek Photos: Franc Šivic, archive Slovenian Beekeepers’ Asscociation Translation and proofreading: Alkemist, prevajalske storitve d.o.o. Financed with funds from the state budget, within the Public Extensions Service on Beekeeping October, 2015 2 CONTENT Work Author Page About Filip Terč Gregor Pivec 8 Propolis and Royal Jelly enhance the antibacterial Bratko Filipič, 10 activity of Honeys against: E. coli, Proteus mirabilis, Lidija Gradišnik, MRSA, St. aureus, Str. pyogenes, Str. agalactiae, Adriana Pereyra, Candida albicans and growth promoting activity on Domen Jaklič, TLT-cell line. Rok Kopinč, Jana Potokar Royal Jelly and Propolis enhance the antibacterial Rihar Klemen, Dunja Exel Gregorič, 12 activity of Honey against Pseudomonas aeruginosa Malči Božnar, Bratko Filipič Impact of propolis on cryopreservation of Arctic charr Khairi El Battawy, 14 (Salvelinus alpinus) sperm E. Brannas Bee propolis: the immuno-biological properties Bratko Filipčič, 16 Lidija Gradišnik, Adriana Pereyra, Jana Potokar Bee products as therapeutic target for pathologies Badiaa Lyoussi 18 Enzymes, honey and health Stane Klemenčič 19 Chemical composition and content Nataša Lilek 22 of free tryptophan in Slovenian bee pollen Adriana Pereyra Gonzales, Janko Božič, Andreja Kandolf Jasna Bertoncelj Biological activity of bee bread Blaž Podrižnik 24 Bee bread– Various usage of bee bread and clinical Pushpendra Singh Bhandari, 26 experiences Mirela Strant Nutritional and functional properties of bee pollen Maria Campos, 27 Ofelia Anjos Aerosol apitherapy devices, Recipe for honey with Borut Gosar 30 pine and spruce tips Massage in apitherapy Sandra Rebek Gašperšič 31 Beekeeping at the Work and Care Centre of the Dolfka Zvone Konstantinovič, Nina Korošec 32 Boštjančič Training, Occupation and Care Centre Mladenović Draga Apitherapy at the Črnomelj Occupational Activity Dušan Milinkovič 34 Centre Apitherapy in a school bee house Blaž Lovrič 36 Bee venom therapy in the past and in the future János Körmendy-Rácz 37 Plantar and vulgar wart treatments with bee venom Ramona Grosu, 38 microapipuncture Alina Varadi Apilarnil Alina Varadi, 39 Cristina Aoșan First steps for good beekeeping practices – guide for Etienne Bruneau 40 apitherapy products Honey and other bee products in primary, secondary, Krešimir Lončar, 41 tertiary and quaternary Mirna Ivandić Lončar, prevention in family medicine Josip Lončar My experience in the treatment of chronic wounds Verica Milojković 43 Oftalmo apitherapy Ramona Grosu, 44 Case study: Superficial Punctured Keratitis Alina Varadi Bee pollen diet Alina Varadi 45 Bee Hive Air therapies in Central-Europe János Körmendy- 47 Rácz Apilarnil – a remarkable bees product and another Cristina Aoșan 48 forgotten Romanian invention 3 PROGRAMME 23rd OCTOBER 2015 Library LUKOVICA Time Session 1 9:00-9:30 Sign-In 9:30-10:00 Welcome Boštjan Noč 10:00-10:30 Propolis and Royal Jelly enhance the Bratko Filipič, Slovenia antibacterial activity of Honeys against: E. coli, Lidija Gradišnik, Proteus mirabilis, MRSA, St. aureus, Str. Adriana Pereyra, pyogenes, Str. agalactiae, Candida albicans and Domen Jaklič, growth promoting activity on TLT-cell line. Rok Kopinč, Jana Potokar 10:30-10:45 Royal Jelly and Propolis enhance the Rihar Klemen, Dunja Slovenia antibacterial activity of Honey against Exel Gregorič, Malči Pseudomonas aeruginosa Božnar, Bratko Filipič 10:45-11:15 Impact of propolis on cryopreservation of Khairi El Battawy, Egypt Arctic charr (Salvelinus alpinus) sperm E. Brannas 11:15-11:40 Bee propolis: the immuno-biological properties Bratko Filipčič, Slovenia Lidija Gradišnik, Adriana Pereyra, Jana Potokar 11:40-12:10 Bee products as therapeutic target for Badiaa Lyoussi Morocco pathologies 12:10-14:00 Lunch with visit to Beekeeping centre of Slovenia Time Session 2 Anton Janša Hall 14:00-14:25 Enzymes, honey and health Stane Klemenčič Slovenia 14:25-14:45 Chemical composition and content Nataša Lilek Adriana Slovenia of free tryptophan in Slovenian bee pollen Pereyra Gonzales, Janko Božič, Andreja Kandolf Jasna Bertoncelj 14:45-15:15 Biological activity of bee bread Blaž Podrižnik Slovenia 15:15-15:45 Bee bread– Various usage of bee bread and Pushpendra Singh India clinical experiences Bhandari, Mirela Strant 15:45-16:15 Coffee break 16:15-17:15 Nutritional and functional properties of bee Maria Campos, Portugal pollen Ofelia Anjos 17:15-17:35 Aerosol apitherapy devices, Recipe for honey Borut Gosar Slovenia with pine and spruce tips 17:35-18:00 Massage in apitherapy Sandra Rebek Gašperšič Slovenia 18:00-18:20 Apitherapy at the Črnomelj Occupational Dušan Milinkovič Slovenia Activity Centre 18:20-18:40 Beekeeping at the Work and Care Centre of the Zvone Konstantinovič, Slovenia Dolfka Boštjančič Training, Occupation and Nina Korošec Care Centre Draga Mladenović 18:40-18.55 Apitherapy in a school bee house Blaž Lovrič Slovenia 18:55-19:25 Degustation /Tasting bee products 19:30-21:30 Social dinner 4 24th OCTOBER 2015 MARIBOR Time Session 1 9:30-9:45 About Filip Terč Gregor Pivec Slovenia 9:45-10:10 Bee venom therapy in the past and in the János Körmendy-Rácz Hungary future 10:10-10:40 Plantar and vulgar wart treatments with bee Ramona Grosu, Romania venom microapipuncture Alina Varadi 10:40-11:05 Apilarnil Alina Varadi, Romania Cristina Aoșan 11:05-11:30 Coffee break 11:30-12:15 First steps for good beekeeping practices – Etienne Bruneau Belgium guide for apitherapy products 12:15-12:45 Honey and other bee products in primary, Krešimir Lončar, Croatia secondary, tertiary and quaternary Mirna Ivandić Lončar, prevention in family medicine Josip Lončar 12:45-13:15 My experience in the treatment of chronic Verica Milojković Serbia wounds 13:15-13:45 Oftalmo apitherapy Ramona Grosu, Romania Case study: Superficial Punctured Keratitis Alina Varadi 13:45-15:30 Lunch Time Session 2 15:30-16:30 Ceremony at the grave of Dr Filip Terč, the Father of Modern Apitherapy 16:30-17:00 Bee pollen diet Alina Varadi Romania 17:00-17:25 Bee Hive Air therapies in Central-Europe János Körmendy- Hungary Rácz 17:25-18:15 Round table about Apitherapy course 18:15-18:45 Awarding of certificate 18:45 Closing 5 Symposium of Apitherapy in Slovenia in the name of Filip Terč Filip Terč, the father of modern apitherapy, was born in Prapořištĕ, a tiny village in the Czech Republic. He lived and worked in Maribor, Slovenia. The more modern study of apitherapy, specifically bee venom, was initiated through the efforts of Phillip Terc in his published work "Report on a Peculiar Connection Between Bee stings and Rheumatism" in 1888. In 2006, after an initiative from the organization Bees for Life – World Apitherapy Network Inc., March 30 was celebrated as “World Apitherapy Day” for the first time to honour the pioneering scientific research of Dr. Filip Terč, the “Father of Modern Apitherapy”, and his professional integrity. In Slovenia, we would like to honour his work with the Symposium of Apitherapy, which takes place in Lukovica, where headquarters of the Slovenian Beekeepeers Association is located, and in Maribor, the town of Filip Terč. In Slovenia we would like to honour the memory of Filip Terč with an annual apitherapy symposium. Boštjan Noč President of Slovenian Beekeepers’ Association 6 Dr. Filip Terč 7 Filip Terč Gregor Pivec Despite the lack of support from his profession, Dr Filip Terč, a physician of Czech descent who lived and worked in Maribor, was able to overcome the boundaries of the strict medical mentality. He was always looking for new ways to treat his patients. He used bees and their products, especially their venom, as a basis to develop new types of treatment. He described his clinical experiences in a medical journal in 1888 (Wiener medizinische Presse). With the declaration of 30 March, Dr Filip Terč’s birthday, as the World Apitherapy Day, his professional efforts finally gained a suitable epilogue. The international apitherapy symposium held at Hompoš Castle in Maribor in March 2012 sparked a book on Dr Terč’s life and work. Written by Dr Gregor Pivec, a physician and amateur beekeeper himself as well as an expert on the history of medicine, the book draws on professional literature, archival sources and the recollections of his family members to present the significance of Dr Terč’s work. It first outlines the beginnings of apitherapy and discusses the symbolic role of bees in the history of mankind, and then it goes on to present Dr Terč’s life, his medical, beekeeping, teaching and popular science work as well as his social life and efforts. Dr Pivec’s book reveals the complexity of Dr Terč’s personality, with reference not only to his professional endeavours but also the universal human values embedded in the politico-economic situation in the Austro-Hungarian monarchy at the time. 8 Anton Janša’s bee house 9 Propolis and Royal Jelly enhance the antibacterial activity of Honeys against: E. coli, Proteus mirabilis, MRSA, St. aureus, Str. pyogenes, Str. agalactiae, Candida albicans and growth promoting activity on TLT-cell line. Bratko Filipič1, Lidija Gradišnik2, Adriana Pereyra3, Domen Jaklič3, Rok Kopinč3, Jana Potokar3 1 Institute for Microbiology and Immunology, Medical Faculty Ljubljana, University of Ljubljana, Zaloška 4, 1105 Ljubljana, Slovenia 2 Institute for Biomedical Sciences, Medical Faculty Maribor, University of Maribor, Taborska 8, 2000 Maribor, Slovenia 3 MEDEX D.o.o., Linhartova 049, 1000 Ljubljana, Slovenia Recently, a marked increase in antibiotic resistance by pathogenic bacteria was found. Such an increase is due to overuse and misuse of antibiotics and has resulted in several highly resistant strains of bacteria, such as MRSA. Attention should therefore be placed on natural alternatives to antibiotic treatment such as: Propolis, Royal Jelly(RJ) and different Honeys.