For Research Use Only PRIM1 Polyclonal ANTIBODY www.ptglab.com Catalog Number:10773-1-AP 2 Publications Catalog Number: GenBank Accession Number: Purification Method: Basic Information 10773-1-AP BC005266 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150UL , Concentration: 133 μg/ml by 5557 WB 1:500-1:1000 Bradford method using BSA as the Full Name: IP 0.5-4.0 ug for IP and 1:200-1:1000 standard; primase, DNA, polypeptide 1 (49kDa) for WB Source: IHC 1:20-1:200 Calculated MW: IF 1:50-1:500 Rabbit 50 kDa Isotype: Observed MW: IgG 50 kDa Immunogen Catalog Number: AG1124 Applications Tested Applications: Positive Controls: IF, IHC, IP, WB, ELISA WB : K-562 cells, HeLa cells, Sp2/0 cells Cited Applications: IP : HeLa cells, IHC IHC : human lymphoma tissue, Species Specificity: human, mouse, rat IF : HeLa cells, Cited Species: mouse Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase Background Information complex. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. PRIM1 is a subnuit of this complex. Notable Publications Author Pubmed ID Journal Application Pierre Kunz 30986223 PLoS One IHC Liam C Hunt 31365869 Cell Rep Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data K-562 cells were subjected to SDS PAGE followed Immunohistochemical analysis of paraffin- IP Result of anti-PRIM1 (IP:10773-1-AP, 4ug; by western blot with 10773-1-AP (PRIM1 antibody) embedded human lymphoma using 10773-1-AP Detection:10773-1-AP 1:300) with HeLa cells lysate at dilution of 1:500 incubated at room temperature (PRIM1 antibody) at dilution of 1:100 (under 10x 2800ug. for 1.5 hours. lens). Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10773-1-AP (PRIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)..