Anti-mIL -28b-IgG Neutralizing monoclonal mouse antibody against mouse interleukin 28B Catalog # mabg-mil28b, mabg-mil28b-5 For research use only, not for diagnostic or therapeutic use Version # 15F01-MM PRODUCT INFORMATION DESCRIPTION Content: Anti-mIL-28b-IgG purified antibody is provided azide-free Anti-mIL-28b-IgG is a fully mouse monoclonal antibody against and lyophilized. It is available in two pack sizes: mIL -28B. This autoantibody was raised in mice by a proprietary - 100 µg: mabg-mil28b method designed to induce the production of anti -cytokine antibodies - 5 x 100 µg: mabg-mil28b-5 directly in the animal. Anti-mIL-28b-IgG has been selected for its Target: natural and recombinant mouse interleukin-28B (IL-28B) ability to efficiently neutralize the biological activity of mIL-28B . Specificity: Reacts with mouse IL-28A and mouse IL-28B. This antibody is produced in hybridomas and purified by affinity No cross -reactivity with human IL-28A or human IL-28B. chromatography. Clone: 3C11 Isotype: Mouse IgG1 APPLICATIONS Immunogen: Mouse IL-28B protein expressed in Swiss mice Anti-mIL-28b-IgG is a neutralizing antibody, it blocks mIL-28-induced following DNA immunization m cellular activation in vitro , as described below. Furthermore, as Formulation: 0.2 m filtered solution in a sodium phosphate buffer anti -mIL-28b-IgG is a mouse anti -mouse antibody, it could be used for with glycine, saccharose and stabilizing agents neutralization assays in vivo . Antibody resuspension Add 1 ml of sterile water per vial to obtain a concentration of 0.1 mg/ml. Neutralization Storage The exact concentration of antibody required to neutralize mIL-28 - Product is shipped at room temperature. Store lyophilized antibody activity is dependent on the cytokine concentration, cell type and at -20 °C. Product is stable for at least 1 year. growth conditions. InvivoGen has determined the neutralization dose - Reconstituted antibody is stable for 1 month when stored at 4 °C and for this antibody using recombinant mIL-28B and HEK -Blue ™ IFN -a/b for 1 year when aliquoted and stored at -20 °C. Avoid repeated cells . These cells were generated by stable transfection of HEK293 freeze-thaw cycles. cells with the mouse STAT2 and IRF9 genes to obtain a fully active Quality control type I IFN signaling pathway. They were further transfected with a - This product has been validated for neutralization using cellular assays. SEAP (secreted embryonic alkaline phosphatase) reporter gene under - The absence of bacterial contamination (e.g. lipoproteins and the control of the IFN- α/β inducible ISG54 promoter . endotoxins) has been confirmed using HEK-Blue ™ TLR2 and m ™ Anti-mIL-28b-IgG (10 ng-1 g/ml) and a negative control antibody HEK -Blue TLR4 cells. (e.g. Mouse IgG1 Control which targets E. coli β-galactosidase) were incubated with recombinant mIL-28B at 10 ng/ml for 30 min prior to BACKGROUND the addition of the HEK-Blue ™ IFN- a/b cells . Neutralization of Interleukin-28 (IL-28) is a member of the type III interferon (IFN IL -28B-induced signaling by anti-mIL-28b-IgG was determined after lambda) cytokine family that exhibits several common features with a 24-hour incubation by assessing SEAP production using 1, 2 type I IFNs: antiviral activity and antitumor activity . IL-28 exerts its QUANTI -Blue ™, a SEAP detection reagent. QUANTI-Blue ™ turns action following binding to a heterodimeric protein complex blue following cytokine stimulation but remains pink if neutralization composed of two subunits, IL-28 receptor alpha and IL-10 receptor occurs. SEAP levels can be assessed by the naked eye or beta, leading to signaling through the Jak/Stat pathway and inducing spectrophotometrically by reading the optical density at 620 -655 nm. the expression of IFN-stimulated genes. IL-28 comes in two isoforms, IL-28A (also known as interferon lambda 2) and IL -28B (also known RELATED PRODUCTS as interferon lambda 3) 1. The IL -28A isoform has been implicated in the promotion of Th1 cell differentiation and the inhibition of Product Catalog Code Th2 -mediated allergic inflammation 3. HEK-Blue ™ IFN- a/b Cells hkb-ifnab 1. Donnelly RP. & Kotenko SV., 2010. Interferon-lambda: a new addition to an old l Mouse IgG1 Control mabg1-ctrlm family. J Interferon Cytokine Res. 30(8):555-64. 2. Li M. et al., 2009. Interferon- s: the ™ modulators of antivirus, antitumor, and immune responses. J. Leukoc. Biol., 86:23-32. QUANTI-Blue rep-qb1 3. Koltsida O. et al., 2011. IL-28A (IFN- λ2 ) modulates lung DC function to promote Th1 immune skewing and suppress allergic airway disease. EMBO Mol Med. 3(6):348-61. TECHNICAL SUPPORT InvivoGen USA (Toll‑Free): 888-457-5873 InvivoGen USA (International): +1 (858) 457-5873 www.invivogen.com InvivoGen Europe: +33 (0) 5-62-71-69-39 InvivoGen Hong Kong: +852 3-622-34-80 E-mail: [email protected].