Proteins Part 2. Proteins White & White — Proteins, Peptides & Amino Acids SourceBook 5 Proteins 4-IBBL Acid Glycoprotein VI, α1- PeproTech 310-11 Human recombinant, expressed in E. coli Synonyms: Orosomucoid MW 19.5k >98% (SDS-PAGE) & HPLC; <0.1 ng endotoxin per Fluka 50646 Human plasma MW 45k ≥98% (GE); 60% mg (1EU/mg); lyophilized | Member of the emerging family of protein; 6% water | Arnaud, P et al, Meth Enzymol, 163: 418, ligands with structural homology to tumor necrosis factor; 185 AA; 1988; Eap, CB & Baumann, P, Electrophoresis, 9: 650, 1988 ED50 < 10ng/mL; SA determined by the dose-dependent stimulation of IL-8 production by human PBMC Acid Glycoprotein, α1- α 6CKINE Synonyms: Orosomucoid; Seromucoid, 1- Synonyms: Exodus II; SLC; T-Cell Activation Gene IV Fluka 50647 Bovine serum ≥99% (gel electrophoresis); ≤5% Biodesign A52013M E. coli Purified | Species specificity: water; ~70% protein mouse Biodesign A50106H Human Purified Biodesign A52035H E. coli Purified Fitzgerald 30-AA01 Human plasma High purity | Chemicon GF086 Human ≥95% Orosomucoid ICN 153905 Human plasma MW 44.1k Lyophilized, salt Biogenesis 4396-0150 Human r-DNA Lyophilized free, >99% BioSource International PHC1474 Human recombinant ICN 191348 Human plasma MW 44.1k Lyophilized, salt PeproTech 300-35 Human recombinant, expressed in E. coli free, >99% (SDS-PAGE) | Negative for HBs Ag & HIV antibodies; MW 12.2k >98% (SDS-PAGE) & HPLC; <0.1 ng endotoxin per Schmid, K etal, Biochemistry, 12:2711, 1973 mg (1EU/mg); lyophilized | In the CC chemokine family; similar to Scipac P153-0 Orosomucoid, from serum/plasma >99%; Exodus-1 (aka MIP-3α & LARC); inhibits hemopoiesis & stimulating lyophilized | Acute phase serum protein chemotaxis; 111 AA; SA determined by its ability to chemoattract total lymphocyte population Scipac P153-1 Orosomucoid, from serum/plasma >96%; lyophilized | Acute phase serum protein Sigma C 0720 Human recombinant, expressed in E. coli Lyophilized from 30% acetonitrile/0.1% TFA containing 1.25 BSA Scipac P153-2 Serum/plasma 40-90%; lyophilized | Serum protein Biogenesis 4396-0350 Mouse r-DNA Lyophilized Sigma C 0845 Mouse recombinant, expressed in E. coli Lyophilized from 30% acetonitrile/0.1% TFA containing 1.25 BSA | Actibind K Endotoxin tested; cell culture tested; long-term growth & ICN 797091/797092 Streptococcus Protein from a variant metabolism of most mammalian cells in culture require that growth of Streptococcal protein L that binds κ light chains &/or the Fab factors, hormones & other factors be present in the basal medium; portion of immunoglobulins of all types; useful in the purification of these factors are provided by fetal bovine serum &/or other sera monoclonal Ab & F Ab fragments routinely used in cell culture protocols; endotoxin tested; cell ab culture tested PeproTech 250-13 Murine recombinant, expressed in E. coli Actin MW 12.0k >98% (SDS-PAGE) & HPLC; <0.1 ng endotoxin per ICN 771012 MW 43k Purified | Suitable for use as a high mg (1EU/mg); lyophilized | In the CC chemokine family; similar to MW marker or as a standard; may be used as an antigen for Ab Exodus-1 (MIP-3a/LARC); inhibits hemopoiesis & stimulating development chemotaxis 110 AA; SA determined by its ability to chemoattract Fluka 01813 Bovine muscle ≥90% (gel electrophoresis); total murine T cell population ≥99% protein; white powder Cortex CP1110 Rabbit >95% Abrin Toxin A Biodesign A08001R Rabbit muscle Purified Sigma L 2017 Abrus precatorius (Jequirity bean) Highly Biogenesis 0070-2504 Rabbit muscle MW 46k Purified; purified by affinity & ion exchange chromatography; solution in 50% glycerol; liquid | Stimulates myosin ATPase activity if first 0.01 M potassium phosphate, pH 7.2, containing 0.15 M NaCl & polymerized 0.1% sodium azide; activity: >50 µg/mL (the lowest concentration Fluka 01812 Rabbit muscle MW ~43k ~96% (gel to agglutinate a 2% suspension of human erythrocytes after 1 hr at electrophoresis) | One of the major proteins of muscle & an 25°C) | Lectin important component of all eukaryotic cells; useful as a reference standard for SDS-PAGE & 2D-electrophoresis; role in muscle Abrin Toxin A & C contraction & relaxation, also involved in a variety of cellular events: cell movement, cytokinesis, chromosome movement, Sigma L 9633 Abrus precatorius (Jequirity bean) Highly phagocytosis & exocytosis; Pudich, J, Watt, S, JBC, 246: 4866, purified by affinity chromatography according to the method of 1971; Vandekerckhove, J & Weber, K, Eur J Biochem, 90: 451, Wei, CH et al, J Biol Chem, 249: 3061, 1974; solution in 0.01 M 1978; Korn, ED, Physiol Rev, 62: 672, 1982; Korn, ED, PNAS, 75: potassium phosphate, pH 7.2, containing 0.15 M NaCl & 0.1% 588, 1978 sodium azide; activity: <60 µg/mL (the lowest concentration to µ µ agglutinate a 2% suspension of human erythrocytes after 1 hr at ICN 159848 Rabbit muscle 3 g/g inhibits 1 g of DNAse I; 25°C) | Lectin >90% | Prepared by a slightly different procedure (reference below); can be used in cell transport & motion studies; inhibits DNAse I & possibly controls DNAse I nucleolytic activity during the Abrin Toxin C cell cycle; Spudich, JA & S Watt, J Biol Chem, 246:4866, 1971 Sigma L 1892 Abrus precatorius (Jequirity bean) Highly purified by affinity & ion exchange chromatography; solution in Actinin 0.01 M potassium phosphate, pH 7.2, containing 0.15 M NaCl & 0.1% sodium azide; activity: <60 µg/mL (the lowest concentration Biodesign A08002C Chicken gizzard Purified to agglutinate a 2% suspension of human erythrocytes after 1 hr at 25°C) | Lectin; this is a further purification of Sigma L 9633 to Actinin, α- eliminate the abrin A content Sigma A 9776 Chicken Gizzard MW ~100k ~80% (SDS- PAGE); suspension in 2 M (NH4)2SO4 containing 20 mM Tris Acetylcholine Transporter Protein acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β- Biotrend 0030-5059 Rat, synthetic >80% | Antigen mercaptoethanol & 1 mM phenylmethylsulfonyl fluoride | Prepared using a modification of the procedure of Neidel, JE & Cuatrecasas, P, Biochem Biophys Res Commun, 91: 152, 1979 6 User's Guide pp. 1-2, Glossary/Indexes pp. 963-1063 Proteins Actinomycin C Aequorin Synonyms: Actinomycin C1; Actinomycin C2; Actinomycin C3 ICN 194084 Jellyfish (Aequorea sp.) Purified powder | A Sigma A 4639 Streptomyces chrysomallus ~95% (HPLC) | bioluminescent protein used in immunoassay procedures; reported to measure calcium serum & subcellular organelle levels <10 µM; Mixture of actinomycins C1, C2, & C3 Izutsu, KT & SP Felton, Clin Chem, 18:77, 1972 Actinomycin D Aequorin Type III Synonyms: Actinomycin IV; Actinomycin C1 Sigma A 4140 Aequorea species (jellyfish) ~0.5% protein Sigma A 1410 Streptomyces species FW 1255.4 ~98% (HPLC); | Protein antibiotic that inhibits cell proliferation by (Bradford) in buffer salts consisting mainly of sodium EDTA, pH 7.5 | A bioluminescent protein; used to measure physiological levels of forming a stable complex with double-stranded DNA & inhibiting µ RNA synthesis calcium in serum & subcellular organelles down to 10 M; 1 mg solid will yield ~200 calcium assays; Izutsu, KT & Felton, SP, Clin Sigma A 4262 Streptomyces species FW 1255.4 ~95% Chem, 18: 77 (1972); Azzi, A & Chance, B, Biochim Biophys Acta, (HPLC); 189: 141 (1969) Actinomycin D, Mannitol 3 Aflatoxin B1, ( H(G))- Sigma A 5156 Lyophilized powder; each vial contains 1 mg ARC ART-247 MW 312.3 15-30 Ci/mmol; 0.55-1.11 actinomycin D & 49 mg of mannitol | Soluble at 20 mg/mL in TBq/mmol; in methanol | Radiochemical water Aflatoxin B , (8,9-3H)- Actinomycin I 2 ARC ART-617 MW 314.3 20-50 Ci/mmol; 0.74-1.85 Sigma A 3012 Streptomyces antibioticus FW 1263.5 TBq/mmol; in methanol | Radiochemical ~80% (HPLC) Aflatoxin M , (3H(G))- Actinomycin V 1 ARC ART-616 MW 328.3 1-5 Ci/mmol; 37-185 GBq/mmol; in Sigma A 2887 Streptomyces antibioticus FW 1269.4 methanol | Radiochemical ~95% (HPLC) Agglutinin, Abrus precatorius Activin A Synonyms: Abrin; Lectin Sigma A 4839 Bovine recombinant, expressed in E. coli Lyophilized from 40% acetonitrile/0.1% TFA | Endotoxin tested; Sigma L 9758 Abrus precatorius (Jequirity bean) Highly cell culture tested; see Sigma C 0720 purified by affinity chromatography according to the method of Lin, J-Y et al, Toxicon, 19: 41, 1981; solution in 0.01 M potassium phosphate, pH 7.2, containing 0.15 M NaCl & 0.1% sodium azide; Acyl Carrier Protein activity: <2 µg/mL (the lowest concentration to agglutinate a 2% ° Sigma A 7303 Escherichia coli ≥60% pure by electrophoresis suspension of human erythrocytes after 1 hr at 25 C) | Extremely in 1% 2-β-MSH; lyophilized hazardous! Be aware of the risks & familiar with safety procedures before you ruse this product; not blood group specific but has an affinity for D-galactose; seeds of Abrus precatorius contain a Adenovirus Type VI nontoxic agglutinin together with 2 major toxic proteins, Abrin A2 2 USBio A0880-02 Single peak by HPLC; 5 mg/mL protein; in also referred to as Abrin b+c & Abrin C also referred to as Abrin a; MEM buffer | Suitable for antigenic applications in immunological conjugates are prepared from affinity purified lectin; activity is µ protocols expressed in g/mL & is determined from serial dilutions in phosphate buffered saline, pH 7.2 of a 1 mg/mL solution; Tomita, M et al, Experientia, 28: 84, 1972; Wei, CH et al, J Biol Chem, 249: Adenylate Cyclase Toxin 3061, 1974; Lin, J-Y et al, Toxicon, 19: 41, 1981; Olsnes, S et al, J Alexis 630-088 Bordetella pertussis recombinant, expressed in Biol Chem, 249: 803, 1974 E. coli MW ~177k ≥90%; lyophilized powder; soluble in water; reconstitute in