Volume 34 July 1967 PROCEEDINGS of The Helminthologica** •./ l Society* of Washington */ - V- ••.' A semiannual journal of research devoted to Helminthology and all branches of Parasitology Supported in part by the Braytori H. Ransom Memorial Trust Fund . > ,• ; •':'• '•'. '•• .''•_;',•'-.' .'•'•. ;'\n $7.00 a Volume; Foreign, $7.50 ALLEN, R..W., F. D. ENZIE, AND K. S. SAMSON. Trials with Yomesan and Other Selected Chemicals Against Thysanosoma actinioides, the Fringed '•;-'..-. \m of Sheep i I .....I... r; I.................... 195 BABERO, BERT B., AND DIANNA MATTHIAS. 'Protospintra peromtjsci n. sp. (Nematoda: Spiruridea) and Other Helminths from Peromyscus spp. in Nevada ___. ._:-__:_____...„„..,„.„.._.. ._„.__ 255 CASTO, S., AND B. McDANiEL. Helminth Parasitism in Gars from South Texas with a Description of Dichelyne ilepisosteus n. sp. (Nematoda: •; ; Cucullanidae) . -—._„: :___-___r._..:. ..... 'IIJ 187 CLARK, GORDON MARSTON. New Speleognathinae from Central and South American Mammals (Acarina., Trombidiformes) ..... ._^_-.-_-_-__-_..^.... 240 ^Donald Bard McMullen—1903-1967 _.i,_.:._ll_- ._..,__:____j._.._.__._._ L...^A.:.._..... 261 FISCHTHAL, -JACOB H., AND ROBERT' E. KUNTZ. Digenetic Trematodes of Amphibians and Reptiles from Fiji, New Hebrides and British Solomon Islands I ....,-...-.1.....^ „ ..... ^_2__4__, ——>-—-—; :„... 244 ( (Continued on Back Cover) . , Copyright © 2011, The Helminthological Society of Washington THE HELMINTHOLOGICAL SOCIETY OF WASHINGTON THE SOCIETY meets once a month from October through May for the presentation and discussion of papers in any and all branches of parasitology or related sciences. All interested persons are invited to attend. Persons interested in membership in the Helminthological Society of Washington may obtain application blanks from the Corresponding Secretary-Treasurer, Miss Edna M. Buhrer, Belts- ville Parasitological Laboratory, Agricultural Research Center, Beltsville, Maryland 20705. A year's subscription to the Proceedings is included in the annual dues ($6.00). President: MAY BELLE CHITWOOD Vice President: WILLIAM B. DnWITT Corresponding Secretary-Treasurer: EDNA M. BUHRER Assistant Corresponding Secretary-Treasurer: HALSEY H. YEGORS Recording Secretary. ALAN C. PIPKIN Librarian: JUDITH M. HUMPHREY (1962- ) -r. Archivist: WILLARD W. BECKLUND (1967- ) . , -Representative to the Washington Academy of Sciences: AUREL O. FOSTER (1965- ) Representative to the American Society of Parasitologists: CHARLES G. DURBIN (1965- ) Executive Committee Members-at-Large: DAVID R. LINCICOME, 1967 GILBERT F. OTTO, 1968 THE PROCEEDINGS OF THE HELMINTHOLOGICAL SOCIETY OF WASHINGTON THE PROCEEDINGS are published by the ^Helminthological Society of Washington. However, papers need not be presented at a meeting to be published in the Proceedings. Non-members may publish in the Proceedings if they will contribute the full cost of publication. Two issues are published each year in January and July. MANUSCRIPTS may be submitted to any member of the Editorial Committee. Manu- scripts must be typewritten, double spaced, and in finished form. Only the ribbon copy will be accepted for publication; it is accepted with the understanding that it-will be published only in the Proceedings. REPRINTS may be ordered from the PRINTER at the same time the corrected proof is returned to the EDITOR. 5 BACK VOLUMES of the Proceedings are available. Inquiries concerning back volumes and current subscriptions should be directed to: Miss Edna M. Buhrer, Corresponding Secretary- Treasurer. EDITORIAL COMMITTEE FRANCIS G. TROMBA, Editor, 1970 ,''';• Beltsville Parasitological Laboratory Agricultural Research Center Beltsville, Maryland, U.S.A. GILBERT F. OTTO, 1969 WILLIAM J.HARGIS, JR., 1971 University of Maryland Virginia Institute of Marine Science \ College Park, Maryland Gloucester Point, Virginia" . DEWEY J. RASKI, 1970 , N.. , „ , ; ; .ALLEN McINTOSH, 1971 Agricultural Experiment Station Beltsville Parasitological Laboratory •;_-, University of California U.S.D.A. '_-'•••. JESSE R. CHRISTIE, 1968 PAUL R WEINSTEIN, 1969 Experiment Station Institute of Allergy and Infectious Diseases University of Florida • N.I.H., U.S.P.H.S. Copyright © 2011, The Helminthological Society of Washington Proceedings of the Helminthological Society of Washington VOLUME 34 JULY 1967 NUMBER 2 A Revised Definition of the Monogenetic Trematode Genus Dactylogyrus, with Descriptions of Four New Species1 CHARLKS E. PRICE- The first mention of the genus Dactylogyrus ious quotes from Yamaguti (1963) are given. in North America was by Hess (1928) who First, his definition of the genus: reported the presence of undetermined species "Dactylogyridae, Dactylogyrinae. Anchors on the gills of large- and smallmouth basses, supported by a single rod-shaped bar. Vas carp, goldfishes, and "other fishes." The valid- deferens usually (always?) looped around in- ity of this observation is questionable in part, testinal limb. Vesicula seminalis formed by because species of Dactylogyrus generally do mere dilation of vas deferens. Two prostatic not occur on the gills of basses and sunfishes. reservoirs present. Cirrus mostly tubular, with Hess was more correct (1930) when he re- accessory piece. Vagina single, exceptionally ferred to dactylogyrids specifically from the double, may or may not have sclerotized sup- gills of carp and goldfishes, as shown subse- porting structures, with submarginal aperture. quently by Mueller and Van Cleave (1932). Parasites of freshwater teleosts." Descriptive research within this genus in In his key to the Dactylogyrinae, Yamaguti North America began when Mueller and Van (1963) gives as his points of differentiation of Cleave (1932) described D. extensus from the Dactylogyrus "Anchor blades without marked gills of the carp, Cyprinus carpio Linnaeus. change in curvature; bar rod-shaped; marginal Prior to the present publication, 56 species booklets usually of uniform size." of Dactylogyrus had been recorded for this Other aspects of diagnosis can be taken from continent. Of these, Mizelle and his students Yamaguti's description of the subfamily Dac- described 34 species (Mizelle, 1937, 1938, tylogyrinae: "Dactylogyridae: Opisthohaptor 1962; Mizelle and Donahue, 1944; Mizelle with one pair of anchors, supported by one and Klucka, 1953; Mizelle and Price, 1964; or two connecting bars, and with 14 marginal Mizelle and Regensberger, 1945; Monaco and booklets; sclerotized accessory structures of Mizelle, 1955; Price and Mizelle, 1964; Wood haptor absent. Eyes present. Testis and ovary and Mizelle, 1957); Mueller, 17 species (1936, rounded or elongate, former posterior to latter. 1938); Putz and Hoffman, 1 species (1965); Vagina present." Seamster, 3 species (1948, 1960), and Mueller Certain aspects of these diagnostic points and Van Cleave, 1 species (1932). This study warrant examination. adds four new species to the list of North Current diagnoses indicate presence of 14 American Dactylogyrus. hooks in Dactylogyrus. Mizelle and Price (1963), employing phase-contrast microscopy, REVISION OF THE CENERIC DIAGNOSIS showed that in a study of 21 Dactylogyrus species, all but four of them actually possessed An improved generic diagnosis is needed for 16 hooks. These "extra" hooks (designated Dactylogyrus, As a base from which to launch "4A" so as not to interfere with the present the task of synthesizing a new diagnosis, var- system of hook designation of North American forms) are quite small, but are nevertheless 1 This study was supported by the Faculty Research Fund of the Department of Biology, North Texas State University. readily observable with the phase-contrast - Department of Biology, North Texas State University, Denton, Texas 76203. microscope. Both Mizelle and Price feel that 117 Copyright © 2011, The Helminthological Society of Washington 118 PROCEEDINGS OF THE [VOL. 34, No. 2 the presence of these hooks is a constant fea- (and sometimes apparent complete absence) ture of Dactylogyrus, and future descriptions of the ventral bar. of species from this genus should include refer- Reference should perhaps be made to Para- ence to them. This additional pair of hooks is dactylogi/rus Thapar (1948), which genus was not restricted to North American Dactylogyrus relegated to subgeneric status (of Dactylogyrus) members, as was shown by the fact that two by Tripathi (1959). Study of both generic Old World Dactylogyrus species possess them, diagnosis and illustrations indicates that this namely, D. anchor atus (Dujardin, 1845) Wage- genus differs from Dactylogyrus only by the ner, 1857, and D. vastator Nybelin, 1924. presence of an unpaired sclerotized structure The question concerning the number of hap- located centrally in the haptor; Thapar (1948) toral bars present in various species has been termed this structure an "onchium." a troubled area of Dactylogyrus classification The inclusion of members of Neodacty- since the establishment of the problematical logyrus and Paradactylogyrus as species of genus Neodactylogyrus by E. W. Price (1938). Dactylogyrus would seemingly constitute a Yamaguti (1963) accepts this genus as a valid contribution to monogenean taxonomy. By one; he takes many North American parasites establishing new taxa to include forms which originally described as Dactylogyrus species differ from Dactylogyrus by presence of un- and transfers them to Neodactylogyrus. Yama- paired structures in the haptor not only creates guti (1963) gives as the main feature of Neo- unnecessary taxonomic confusion, but