SAN TA C RUZ BI OTEC HNOL OG Y, INC . GPR85 (N-14): sc-54685 BACKGROUND APPLICATIONS G protein-coupled receptors (GPRs), also known as seven transmembrane GPR85 (N-14) is recommended for detection of GPR85 of mouse, rat and receptors, heptahelical receptors or 7TM receptors, comprise a superfamily human origin by Western Blotting (starting dilution 1:200, dilution range of proteins that play a role in many different stimulus-response pathways. 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range G protein-coupled receptors translate extracellular signals into intracellular 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range signals (G protein activation) and they respond to a variety of signaling mol - 1: 30-1:3000). ecules, such as hormones and neurotransmitters. GPR85 (G protein-coupled GPR85 (N-14) is also recommended for detection of GPR85 in additional receptor 85), also designated SREB2 (super conserved receptor expressed in species, including equine, canine, bovine, porcine and avian. brain 2), is a 370 amino acid multi-pass membrane protein that functions as an orphan receptor and belongs to the GPR family. Highly expressed in testis Suitable for use as control antibody for GPR85 siRNA (h): sc-62403, GPR85 and brain, GPR85 is found at lower levels in spleen, placenta and small siRNA (m): sc-62404, GPR85 shRNA Plasmid (h): sc-62403-SH, GPR85 shRNA intestine, and is encoded by a gene that maps to human chromosome 7q31. Plasmid (m): sc-62404-SH, GPR85 shRNA (h) Lentiviral Particles: sc-62403-V and GPR85 shRNA (m) Lentiviral Particles: sc-62404-V. REFERENCES Molecular Weight of GPR85: 42 kDa. 1. Matsumoto, M., et al. 2000. An evolutionarily conserved G protein-coupled Positive Controls: rat hippocampus tissue extract. receptor family, SREB, expressed in the central nervous system. Biochem. Biophys. Res. Commun. 272: 576-582. RECOMMENDED SECONDARY REAGENTS 2. Hellebrand, S., et al. 2000. The brain-specific G protein-coupled receptor To ensure optimal results, the following support (secondary) reagents are GPR85 with identical protein sequence in man and mouse maps to human recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 chromosome 7q31. Biochim. Biophys. Acta 1493: 269-272. (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey 3. Hellebrand, S., et al. 2001. Gpr85, a novel member of the G protein-coupled anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ receptor family, prominently expressed in the developing mouse cerebral Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: cortex. Brain Res. Gene Expr. Patterns 1: 13-16. sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- 4. Jeon, J., et al. 2002. Cloning and localization of rgpr85 encoding rat G 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) protein-coupled receptor. Biochem. Biophys. Res. Commun. 298: 613-618. with UltraCruz™ Mounting Medium: sc-24941. 5. Lee, D.K., et al. 2002. Novel G protein-coupled receptor genes expressed in the brain: continued discovery of important therapeutic targets. Expert STORAGE Opin. Ther. Targets 6: 185-202. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of 6. Lee, D.K., et al. 2003. Continued discovery of ligands for G protein-coupled shipment. Non-hazardous. No MSDS required. receptors. Life Sci. 74: 293-297. RESEARCH USE 7. Matsumoto, M., et al. 2005. A conserved mRNA expression profile of SREB2 (GPR85) in adult human, monkey, and rat forebrain. Brain Res. Mol. For research use only, not for use in diagnostic procedures. Brain Res. 138: 58-69. PROTOCOLS CHROMOSOMAL LOCATION See our web site at www.scbt.com or our catalog for detailed protocols and Genetic locus: GPR85 (human) mapping to 7q31.1; Gpr85 (mouse) mapping to support products. 6 A1. SOURCE GPR85 (N-14) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an N-terminal extracellular domain of GPR85 of human origin. PRODUCT Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-54685 P, (100 µg pep tide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.