Effects of prenatal stress on sepia officinalis Caitlin O Brien To cite this version: Caitlin O Brien. Effects of prenatal stress on sepia officinalis. Animal biology. Normandie Université, 2017. English. NNT : 2017NORMC249. tel-01881106 HAL Id: tel-01881106 https://tel.archives-ouvertes.fr/tel-01881106 Submitted on 25 Sep 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THESE Pour obtenir le diplôme de doctorat Spécialité Physiologie et Biologie des Organismes, Populations, Interactions Préparée au sein de Université de Caen-Normandie Effects of Prenatal Stress on Sepia officinalis Présentée et soutenue par Caitlin Elizabeth O’BRIEN Thèse soutenue publiquement le 08/12/2017 devant le jury composé de Mr Olivier BASUYAUX Docteur, SMEL, Blainville sur Mer Examinateur Mme Aline BERTIN Chargée de Recherches INRA, HDR, Université de Tours Rapporteur Mr Paco BUSTAMANTE Professeur des Universités, Université de LaRochelle Rapporteur Mme Anne-Sophie DARMAILLACQ Maître de Conférences, HDR, Université de Caen-Normandie Examinateur Mme Patrizia D’ETORRE Professeur des Universités, Université de Paris XIII Examinateur Mr Ludovic DICKEL Professeur des Universités, Université de Caen-Normandie Directeur de thèse Mr Jean-Paul ROBIN Professeur des Universités, Université de Caen-Normandie Examinateur Thèse dirigée par Ludovic DICKEL, UMR 6552 EthoS Publications and Submitted Manuscripts 1. O'Brien, C. E., Bowie, M., Billard, P., Darmaillacq, A. S., Jozet-Alves, C., Behaïm, D., Basuyaux, O., & Dickel, L. "The effect of an artificial incubation environment on hatchling size and behavior in the cuttlefish, Sepia officinalis." Vie et Milieu—Life and Environment 66.1 (2016): 97–105. Article #5, Pages 130-148. 2. O'Brien, C. E., Mezrai, N., Darmaillacq, A. S., & Dickel, L. Behavioral development in embryonic and early juvenile cuttlefish (Sepia officinalis). Developmental Psychobiology 9999 (2016): 1–16. Article #2, Pages 36-66. 3. Darmaillacq, A. S., Mezrai, N., O'Brien, C. E., & Dickel, L. Visual ecology and the development of visually guided behavior in the cuttlefish. Frontiers in Physiology, 8 (2017): 1–8. Article #3, Pages 67-81. 4. O’Brien, C. E., Bellanger, C., Jozet-Alves, C., Mezrai, N., Darmaillacq, A. S., & Dickel, L. Effects of Maternal and Embryonic Stress on Egg Production and Offspring in the Cuttlefish, Sepia officinalis. Journal of Experimental Marine Biology and Ecology (Under Revision). Article #1, Pages 11-28. 5. O’Brien, C. E., Jozet-Alves, C., Mezrai, N., Bellanger, C., Darmaillacq, A. S., & Dickel, L. Maternal and Embryonic Stress Influence Offspring Behavior in the Cuttlefish Sepia officinalis. Frontiers in Physiology (Under Revision). Article #4, Pages 82-102. 6. O’Brien, C.E., Roumbedakis, K., Winkelmann, I. The Future of Cephalopod Science: Perspectives from Three Early-Career Researchers. Frontiers in Physiology (In Preparation). Article #6, Pages 159-168. International Congresses Oral Presentations O’Brien, C.E. “Cephalopod Research: Visions of the Future; Behavior, Cognition and Neurobiology.” 2017 COST Action CephsInAction and CIAC meeting (“Cephalopod Science; from Biology to Welfare”) in Heraklion, Crete (Greece), March 28-30, 2017.* *Keynote lecture O’Brien, C.E., Jozet-Alves, C., Darmaillacq A-S., Mezrai, N., Bellanger, C., Dickel L. “Effects of Reproductive Stress on Offspring Behavior in the cuttlefish, Sepia officinalis.” 2017 COST Action CephsInAction and CIAC meeting (“Cephalopod Science; from Biology to Welfare”) in Heraklion, Crete (Greece), March 28-30, 2017. Posters O’Brien, C.E., Bellanger, C., Jozet-Alves, C., Mezrai, N., Darmaillacq A-S., Dickel L. “Reproductive Effects of Maternal and Prenatal Stress in the Cuttlefish, Sepia officinalis.” COST Action CephsInAction and CIAC meeting (“Cephalopod Science; from Biology to Welfare”) in Heraklion, Crete (Greece), March 28-30, 2017. O’Brien, C.E., Bellanger, C., Jozet-Alves, C., Mezrai, N., Darmaillacq A-S., Dickel L. “Reproductive Effects of Maternal and Prenatal Stress in the Cuttlefish, Sepia officinalis.” 2017 L'Ecole doctorale Normande de Biologie Intégrative, Santé et Environnement (EdNBISE) conference in Le Havre, France, March 16-17, 2017. National Congresses Oral Presentations O’Brien, C.E., Jozet-Alves, C., Darmaillacq A-S., Mezrai, N., Bellanger, C., Dickel L. “Effects of maternal stress on behavior in juvenile cuttlefish (Sepia officinalis).” 2016 Société Française pour l’Étude du Comportement Animal (SFECA) conference in Caen, France, March 22-24, 2016.* *recipient of the Castor Prize for best oral presentation O’Brien, C.E., Jozet-Alves, C., Darmaillacq A-S., Mezrai, N., Bellanger, C., Dickel L. “Effects of artificial incubation and prenatal predator exposure on hatchling behavior in Sepia officinalis.” 2015 Société Française pour l’Étude du Comportement Animal (SFECA) conference in Strasbourg, France, April 20- 23, 2015. Posters O’Brien, C.E., Bowie, M., Billard, P., Darmaillacq, A.S., Jozet-Alves, C., Benhaïm, D., Basuyaux, O., Dickel. L. “Embryonic predator-exposure affects visual acuity during the first prey encounter of Sepia officinalis.” 2015 L'Ecole doctorale Normande de Biologie Intégrative, Santé et Environnement (EdNBISE) conference in Le Havre, France, March 16-17, 2017. Acknowledgments First off, I would like to thank my adviser and mentor, Dr. Ludovic Dickel, for reaching out to me four years ago and giving me this life-changing opportunity to pursue my dream of cephalopod research. Your patience and guidance have been immeasurably helpful to this research and my professional development, and I feel incredibly honored and grateful to have had you as a supervisor. Merci. I am also grateful for the guidance of Anne-Sophie Darmaillacq, who generously shared her vast knowledge of ethology and prenatal development with me. Without the wisdom of Christelle Jozet-Alves, I could never have found my way around the marine station or completed a statistical analysis. Thank you for tolerating my constant unscheduled demands on your knowledge! In addition to collaboration and guidance at work, Dr. Cécile Bellanger invited me into her home and family. Dinners and outings with the Bellanger family were a highlight of my week, and made me feel welcome in an unfamiliar country. Merci Cecile, Laurent, Alice, Hugo and Praline. I am also incredibly thankful to have had the aid of Isabelle Chevalier, Nadège Villain-Naud and Céline Thomasse. Merci beaucoup ladies! Je pouvais toujours compter sur vous! At the Centre de Recherches en Environnement Côtier (CREC), Jean Paul Lehodey, David Liegard, Frédéric Guyon and David Lemeille provided logistical support. The Synergie Mer et Littoral (SMEL) was kind enough to host me during several experiments. These experiments benefitted from the assistance of several WONDERFUL technicians, including Sébastien Pien, Vincent Lefebvre, Suzy Moal and Jean-Louis Lesoif. Moreover, Dr. Olivier Basuyaux, as well as Cécile, Yaël Yuna and Yeti Basuyaux generously invited me to stay with them in their home in Blainville while I conducted these experiments. The time I spent in this bucolic heaven-on-earth with la petit famille is definitely the highlight of my three years in France. Merci! Several students of mine became good friends in the course of our time together. Kevin Bairos- Novak, you were the best summer companion I could have asked for! Pauline Billiard, thank you for being such a good sport with a mentor who was new to France and clueless! Camille Auger, I was super impressed with your motivation to learn something outside your chosen field. And Estelle Paupy, you did a tremendous job analyzing neurobiological samples and learning experiments. Other students who provided invaluable assistance with experiments and analysis included Chloe Jane Way, Héloïse Duretz, Alexis Perret, Hannah Cockerton, Hannah Amor and Tim Pakyar. Thank you! My officemates at the university were a source of guidance, laughter and comfort during the three years of my thesis, especially when it came to French to English translation! Flavie Bidel, you were a constant source of support, advice and inspiration! Anne Quiédeville what little French I know is thanks to you and your patience. Greg Beaudet, I miss your random nerf intrusions during slow afternoons. Thanks to the three of you for adopting this wayward American. Katia Hamidouche, thanks for making me laugh whenever I needed it. Apolline Chabenat, you’re a great housemate. Nawel Mezrai, it’s the sun, stupid! Rachel Asselot, thanks for distracting me daily with your dreamy stares. Pierre Lecouflet, thanks for playing the crossword with me. I would also like to thank Alex Schnell, who was a source of professional inspiration and guidance, as well as a weekly bowl of pho. And many thanks as well to Sophie Corvaisier and Marc Pignon for all of the delightful outings and laughter in Bretagne! To all the other wonderful folks at the GMPc, thank you for welcoming me to your lab and tolerating my terrible French! Thanks also to Anna Mazaleyrat and Julie "Pangolin" Fleitz for introducing me to all