ProteinTech Group, Inc 2201 W. Campbell Park Dr. Chicago, IL 60612 Toll Free: 1-888-4PTGLAB In Illinois: 1312-455-8498 FOR IN VITRO RESEARCH USE ONLY Fax: 1-312-455-8408 NOT FOR USE IN HUMANS OR ANIMALS Web: www.ptglab.com Similar to amplified in osteosarcoma OS9；Protein OS-9 precursor BC000532 About the antigen: The antibody is stored at 50% Glycerol in PBS o Three splice variants of OS-9 have been buffer containing 0.02% sodium azide at -20 C. identified, encoding 667, 617 and 597 amino The final concentration generally is 150 µg/ml for acid peptides for isoforms 1, 2 and 3, affinity purified antibody, 1 mg/ml for IgG fraction respectively. The expression of OS-9 is detected from protein A Sepharose. Glycerol was added in all 16 human tissues examined, suggesting to a final concentration of 50% to so that that OS-9 is ubiquitously expressed in human antibodies can be stored at -20 oC without going tissues. The OS-9 gene maps to a region (q13- through freeze-thaw cycles during shipping. 15) of chromosome 12 and is frequently co- amplified with CDK4 in human sarcomas. Northern blotting analyses reveal similar levels of expression of OS-9 gene in various tumor cell lines of sarcoma cells, carcinoma cells and Hela cell lysates were subjected 103 kd to 10% SDS PAGE followed by myeloid leukemia cells, but 3-4 times higher western blot with affinity purified expression in OsA-CL cells and Rh30 cells 70 kd containing a homogeneously staining region of Ab at dilution of 1:400 12q13-15. The function of mammalian OS-9 50 kd protein is not known. The Saccharomyces cerevisiae homolog protein, YOS9, is a membrane-associated glycoprotein that localizes to the endoplasmic reticulum (ER), and interacts genetically with genes involved in ER- Golgi transport. Yos9 plays a direct role in the transport of glycosylphosphatidylinositol (GPI)- anchored proteins to the Golgi apparatus. Yos9 binds directly to Gas1 and Mkc7 and accelerates Gas1 transport and processing in cells overexpressing YOS9. As Yos9 is not a component of the Emp24 complex, it may act as a novel escort factor for GPI-anchored proteins in ER-Golgi transport in yeast, and possibly in mammals. The antibody: Format 1: Affinity purified Catalog No. : 10061-1-AP Weight/Volume: 30 µg/150 µl Tested Applications: WB (1:400) IP: (5 µl/ml lysate) Immunogen: Hu, Partial. Tested Specificity: Mo, Hu, Rb, Rt. Host: Rb Antibody species: Polyclonal Storage Information: .