FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012) FluoroMax®-4 & FluoroMax®-4P with USB Operation Manual http://www.HORIBA.com Rev. D i FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012) Copyright © 2011, 2012 by HORIBA Instruments Incorporated. All rights reserved. No part of this work may be reproduced, stored, in a retrieval system, or transmitted in any form by any means, including electronic or mechanical, photocopying and recording, without prior written permission from HORIBA Instruments Incorporated. Requests for permission should be requested in writing. Origin® is a registered trademark of OriginLab Corporation. Alconox® is a registered trademark of Alconox, Inc. Ludox® is a registered trademark of W.R. Grace and Co. Teflon® is a registered trademark of E.I. du Pont de Nemours and Company. Windows® is a trademark of Microsoft Corpora- tion. Uniblitz® is a registered trademark of VA, Inc. Information in this manual is subject to change without notice, and does not represent a commitment on the part of the vendor. July 2012 Part Number J810005 ii FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012) Table of Contents 0: Introduction ........................................................................................... 0-1 About the FluoroMax®-4 and FluoroMax®-4P .................................................................................. 0-1 Chapter overview ............................................................................................................................. 0-2 Disclaimer ......................................................................................................................................... 0-3 Safety summary ............................................................................................................................... 0-5 Risks of ultraviolet exposure ............................................................................................................ 0-7 Additional risks of xenon lamps ........................................................................................................ 0-9 CE compliance statement .............................................................................................................. 0-11 1: Requirements & Installation ........................................................................ 1-1 Safety-training requirements ............................................................................................................ 1-1 Surface requirements ....................................................................................................................... 1-2 Environmental requirements ............................................................................................................ 1-3 Electrical requirements ..................................................................................................................... 1-4 Unpacking and Installation ............................................................................................................... 1-5 Software emulation .......................................................................................................................... 1-9 2: System Description ................................................................................... 2-1 Introduction ...................................................................................................................................... 2-1 Basic theory of operation ................................................................................................................. 2-1 Optical layout.................................................................................................................................... 2-2 3: System Operation ...................................................................................... 3-1 Introduction ....................................................................................................................................... 3-1 Power switch .................................................................................................................................... 3-1 Turning on the system ...................................................................................................................... 3-2 Checking system performance ......................................................................................................... 3-3 4: Data Acquisition ....................................................................................... 4-1 Introduction to FluorEssence™ and Multigroup ............................................................................... 4-1 Experiment Menu button .................................................................................................................. 4-2 Previous Experiment Setup button .................................................................................................. 4-4 Auto Run Previous Experiment button ............................................................................................. 4-5 Make Overlay File button ................................................................................................................. 4-6 3D Scan to 3D Profile button ............................................................................................................ 4-7 Run JY Batch Experiments button ................................................................................................... 4-9 Real Time Control .......................................................................................................................... 4-11 Create/Use Calibration Curve from CWA Data .............................................................................. 4-12 2D Intensity Map ............................................................................................................................ 4-16 Launch DataStation ........................................................................................................................ 4-18 Switch menu between HJY Software Application and Origin Std. ................................................. 4-19 Multigroup ....................................................................................................................................... 4-20 Running an unknown sample ......................................................................................................... 4-23 Using corrected signals in FluorEssence™ ................................................................................... 4-28 5: Optimizing Data ....................................................................................... 5-1 Cuvette preparation .......................................................................................................................... 5-1 Sample preparation .......................................................................................................................... 5-2 Running a scan on a sample ............................................................................................................ 5-4 Measuring the G factor ..................................................................................................................... 5-5 Improving the signal-to-noise ratio ................................................................................................... 5-7 Correcting data ............................................................................................................................... 5-14 6: Maintenance ............................................................................................ 6-1 Introduction ....................................................................................................................................... 6-1 Lamp replacement ........................................................................................................................... 6-1 Electronics ...................................................................................................................................... 6-11 Updating the FluoroMax®-4 firmware ............................................................................................. 6-13 iii FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012) 7: Troubleshooting........................................................................................ 7-1 Chart ................................................................................................................................................. 7-1 Using diagnostic spectra .................................................................................................................. 7-4 Further assistance… ....................................................................................................................... 7-9 8: Producing Correction Factors ....................................................................... 8-1 Introduction....................................................................................................................................... 8-1 Generating emission correction factors ........................................................................................... 8-2 Calculating emission correction factors ........................................................................................... 8-3 Using correction-factor files...........................................................................................................