CV_SantoshAlluri SANTOSH ALLURI [email protected] [email protected] Columbia, MO, USA, 919-869-9436 PROFILE Experience in organic/radiosynthesis allied to analytics, PET-chemistry & imaging. Seeking a suitable opportunity within translational molecular imaging and nuclear medicine where I can employ and expand my associated knowledge and skills. RESEARCH WORK Post-Doctoral Researcher 01/2019- Missouri University Research Reactor, Columbia, USA (Dr. Kun-Eek Kil) present • Development of precursors and F-18, C-11 radiotracers for Cancer, Cardiac and Neuro- preclinical PET imaging Ph.D. 10/2014- University of Oslo, Oslo, Norway (Dr. Patrick Riss) 12/2018 • Dissertation: Target oriented synthesis for imaging cell-damage linked proteins • Mentoring undergraduates in chemistry laboratory Master’s thesis University of Southern Denmark, Odense, Denmark (Dr. Poul Nielsen) 05/2013- • Synthesis of new triazole analogs of chloramphenicol using click chemistry 11/2013 EDUCATION M.Sc. Structural chemistry and Spectroscopy 04/2012- University of Leipzig, Leipzig, Germany 04/2014 B.Sc. Chemistry, Biochemistry, Biotechnology 08/2006- Osmania University of Arts & Sciences, Hyderabad, India 08/2009 PhD work • Developed [3H]Rucaparib and carried out in vitro autoradiography to map (UiO) PARP protein quantity in brain models • Optimized and scaled-up 2-step manual/automated radiosynthesis of System - 18 xC inhibitor 5-[ F]fluoro-L-amino suberate in Scintomics HBIII module and participated in its validation via tumour-PET • Developed various aziridine precursors and optimized ring-opening conditions for β-[18F/19F]fluoro-α-amino acids specific to NMDA receptors • Involved in precursors synthesis for 11C-carboxylation/methylation projects Post-Doctoral • Developing synthesis methods for precursors to be labelled with F-18, C-11 work (MU) • Developing 1 – 3 step radiosynthesis (F-18, semi-automation), HPLC, QC methods towards novel PET-tracers for PD-L1, CXCR3, Alpha-2C systems • Production of Sigma-1 receptor tracer [18F]FTC-146 for dog-PET • Involvement in in vitro & in vivo validation of new radiotracers CV_SantoshAlluri Related skills • Multi-step organic synthesis, compound separation/purification • Acquisition & interpretation of NMR, HPLC, MS data • Development of prep/analytical-HPLC (radio), quality control & formulation methods for reference standards and PET-radiotracers • Perform in vitro assays to support in vivo data • Detailed synthesis procedures, production protocols (SOP/GLP) Organizational • Developed competences in multi-tasking in day-to-day work skills • Coordinate with colleagues, exchange ideas • Able to work in team as well as independently • Used to work in interdisciplinary/collaborative work setting • Able to consider & analyze different opinions and take the lead when necessary Fellowships • PhD fellowship- Department of Chemistry, University of Oslo (4 years) • Master’s thesis- ERASMUS exchange program fellowship (6 months) Proposals • Wrote a proposal titled “Development of PET tracers for oxidative cell damage” as a part of PhD application to the University of Oslo, Norway • Participated in grant proposal for a project entitled “Development of Subtype Specific Radiotracers for Brain Adrenergic Receptors” PEER reviewed Publications 1. S R Alluri, S W Kim, N D Volkow, K E Kil (2020) PET Radiotracers for CNS-Adrenergic Receptors: Developments and Perspectives. Molecules, 25 (17), 4017. PMID: 32899124 2. S R Alluri, K Pitman, E Malinen, P J Riss (2020) Synthesis, radiosynthesis and positron emission tomography neuroimaging using 5‐[18F] fluoro‐L‐amino suberate. Journal of labelled compounds and radiopharmaceuticals, 63, 6-14. PMID: 31697846 3. K E Pitman, S R Alluri, A Kristian, H Lyng, P J Riss, E Malinen (2019) Influx rate of 18F-fluoro-amino suberic acid reflects cystine/glutamate antiporter expression in tumour xenografts. European Journal of Nuclear Medicine, 46 (10), 2190. PMID: 31264167 4. S R Alluri, P J Riss (2018) Stereospecific radiosynthesis of 3-fluoro amino acids: access to enantiomerically pure radioligands for positron emission tomography. Organic & Biomolecular Chemistry, 16(13) 2219-24. PMID: 29546903 5. S R Alluri, P J Riss (2018) Poly (ADP-ribose) polymerase in neurodegeneration: radiosynthesis and radioligand binding in ARC-SWE tg mice. ACS Chemical Neuroscience, 9(6) 1259. PMID: 29544053 Manuscripts in progress ▪ S R Alluri, Y Higashi, L D Watkinson, T Hoffman, C Bysani, K E Kil, Development of a Small-molecule CXCR3 PET Radiotracer for Atherosclerosis Imaging ▪ S R Alluri, S Eisenberg, S W Kim, N D Volkow, K E Kil: Synthesis and PET-studies of novel radiotracers for alpha-2C receptor imaging CV_SantoshAlluri Conferences • (Attendance) Symposium on Biomolecular Synthesis and Nanotechnology, University of Southern Denmark, Denmark (06/2013) • (Poster) SR Alluri, P J Riss, Oxidative stress and PET, European association of nuclear medicine, Gothenburg, Sweden (10/2014) • (Seminar) Synthesis, radiosynthesis of 5-fluoro amino-L-suberate, Strategic Research Initiatives, Sundvolden, Norway (02/2015) • (Seminar) Imaging DNA damage, PhD day 2017, University of Oslo, Norway (06/2017) • (Poster) Development of Small molecule CXCR3-PET tracer, University of Missouri Cardiovascular Day 2020, USA (02/2020) • (Abstract) S R Alluri, Y Higashi, L D Watkinson, T Hoffman, C Bysani, K E Kil, Development of Small molecule CXCR3-PET tracer, SNMMI-06/2020, USA COVER LETTER Subject: To offer my candidature for post-doc at the Department of Radiology and Medical Imaging at the University of Virginia (UVA). Dear Dr. Kiel Neumann, I am Santosh Alluri, currently working as an early post-doctoral researcher under Dr. Kun-eek Kil at the Missouri University Research Reactor, MO, USA. My motivation for further research/experience in chemistry got something to do with my career and interdisciplinary knowledge. My graduate studies had given me proper theoretical gain in chemistry with a focus on organic chemistry. I have also acquired knowledge and skills of radiochemistry during practical exposures at various research facilities. I have completed my PhD under Dr. Patrick Riss at University of Oslo, Norway. The dissertation work was focused on development of PET-probes - specific to PARP, NMDA and System xc and link them in a complex cell-damage process. In addition, the work provided me scope in research related to brain disorders and cancer. The following overview of research expertise supplements my research interests. • Target-oriented multi-step organic synthesis¸ analytics (TLC, IR, NMR, MS, HPLC) • Familiar with design, development, quality control and validation of radiotracers and variety of radiolabeling techniques for PET-application • Experience in optimization and scale-up of radiosynthesis procedures • Skill in in vitro autoradiography, microscale thermophoresis • Ability to write scientific articles, detailed experimental protocols • Good in handling software and tools that could accelerate quality research • Independent/team player with good presentation skills CV_SantoshAlluri As a post-doctoral fellow at MU, I am engaged in different projects that involve development of various small and large molecule precursors for C-11 and F-18 radiolabeling. I am also responsible for development of 2 – 3 step radiosynthesis, purification, quality control methods towards novel PET tracers specific to CXCR3, Alpha-2C and PD-L1 systems. In addition, I am performing in vitro assays to supplement PET-imaging results. I wish to pursue my career in the field of translational molecular imaging and nuclear medicine where I can utilize and advance my existing knowledge and skills. I am interested in getting more exposed to tracer automation, translation, animal handling and PET-technique. My experience at different universities taught me to coordinate my efforts with other motivated individuals. I am certain that these experiences, abilities would serve me well in your group and projects and I hope I can cope up with new methodologies, techniques quickly. I would be happy to provide if any additional information is needed and be pleased if my profile is considered for the position. I look forward to hearing from you. Sincerely, Santosh Alluri [email protected] 919-869-9436 .