Leader in Biomolecular Solutions for Life Science HNRNPF Rabbit pAb Catalog No.: A5505 Basic Information Background Catalog No. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear A5505 ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in Observed MW the nucleus and regulate alternative splicing, polyadenylation, and other aspects of 47kDa mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have Calculated MW distinct nucleic acid binding properties. The protein encoded by this gene has three 45kDa repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced Category variants, encoding the same protein, have been identified. Primary antibody Applications WB, IF Cross-Reactivity Human, Mouse Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 3185 P52597 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPF (NP_001091678.1). Synonyms HNRNPF;HNRPF;OK/SW-cl.23;mcs94-1 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using HNRNPF antibody (A5505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. Immunofluorescence analysis of U2OS cells using HNRNPF antibody (A5505). Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..