Clinical and Experimental Pharmacology and Physiology (2005) 32, 7–12 NEUROTOXIC EFFECTS OF VENOMS FROM SEVEN SPECIES OF AUSTRALASIAN BLACK SNAKES (PSEUDECHIS): EFFICACY OF BLACK AND TIGER SNAKE ANTIVENOMS Sharmaine Ramasamy,* Bryan G Fry† and Wayne C Hodgson* *Monash Venom Group, Department of Pharmacology, Monash University, Clayton and †Australian Venom Research Unit, Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia SUMMARY the sole clad of venomous snakes capable of inflicting bites of medical importance in the region.1–3 The Pseudechis genus (black 1. Pseudechis species (black snakes) are among the most snakes) is one of the most widespread, occupying temperate, widespread venomous snakes in Australia. Despite this, very desert and tropical habitats and ranging in size from 1 to 3 m. little is known about the potency of their venoms or the efficacy Pseudechis australis is one of the largest venomous snakes found of the antivenoms used to treat systemic envenomation by these in Australia and is responsible for the vast majority of black snake snakes. The present study investigated the in vitro neurotoxicity envenomations. As such, the venom of P. australis has been the of venoms from seven Australasian Pseudechis species and most extensively studied and is used in the production of black determined the efficacy of black and tiger snake antivenoms snake antivenom. It has been documented that a number of other against this activity. Pseudechis from the Australasian region can cause lethal 2. All venoms (10 ␮g/mL) significantly inhibited indirect envenomation.4 twitches of the chick biventer cervicis nerve–muscle prepar- The envenomation syndrome produced by Pseudechis species ation and responses to exogenous acetylcholine (ACh; varies across the genus and is difficult to characterize because the 1 mmol/L), but not to KCl (40 mmol/L), indicating activity at offending snake is often not identified.3,5 However, symptoms of post-synaptic nicotinic receptors on the skeletal muscle. envenomation may include local pain, generalized itchiness, 3. Prior administration of either black or tiger snake sweating, faintness, nausea, vomiting, prostration and headache.5 antivenom (5 U/mL) prevented the inhibitory effects of all Phospholipase A2 (PLA2) toxins have been isolated from Pseudechis venoms. P. australis,6,7 P. colletti,8 P. porphyriacus9 and P. papuanus.10 In 9,11 6,7 4. Black snake antivenom (5 U/mL) added at t90 (i.e. the addition, pre- and post-synaptic neurotoxins, platelet time-point at which the original twitch height was reduced by inhibitors12 and myotoxic,13–15 pro-16 and anticoagulating,17 anti- significantly reversed the effects of P. butleri (28 ؎ 5%), bacterial,18 haemorrhagic16 and necrotic5 activities have been (90% .P. guttatus (25 ؎ 8%) and P. porphyriacus (28 ؎ 10%) venoms. demonstrated for Pseudechis venoms Tiger snake antivenom (5 U/mL) added at the t90 time-point Recommended treatment following black snake envenomation significantly reversed the neurotoxic effects of P. guttatus includes application of a pressure immobilization bandage P. papuanus (47 ؎ 5%) and P. porphyriacus immediately after the bite and, depending on the severity of the ,(4% ؎ 51) venoms. envenomation, black snake antivenom.19,20 Although, tiger snake (7% ؎ 20) 5. We show, for the first time, the presence of neurotoxins in antivenom is recommended for systemic P. porphyriacus enveno- the venom of these related snake species and that this activity mation, it is not indicated for use in the case of P. australis or is differentially affected by either black snake or tiger snake P. colletti envenomation.21 The pharmacological basis for the antivenoms. apparent cross-reactivity of tiger snake antivenom against venoms Key words: antivenom, elapid, king brown, mulga, neuro- from snakes of different genera is unknown. To date, there has been toxicity, neurotoxin, Pseudechis, venom. no examination of the efficacy of black or tiger snake antivenom to neutralize the activity of Pseudechis venoms. Therefore, in the present study, we investigated the in vitro neurotoxicity of venoms INTRODUCTION from seven Pseudechis species and the ability of black or tiger snake antivenom to neutralize these effects. Elapid snakes form a conspicuous component of the herpetofauna of Australasia and represent the majority of snakes found in METHODS Australia. In addition, these snakes are of special significance as Snake collection and venom preparation The following venoms were purchased from Venom Supplies (Tanunda, Correspondence: Associate Professor Wayne C Hodgson, Department of SA, Australia): P. australis (mulga snake) from Eyre Penninsula, P. colletti Pharmacology, Monash University, Victoria 3800, Australia. (Collett’s snake) from central Queensland; P. guttatus (spotted black snake) Email: [email protected] from south-east Queensland; P. papuanus (Papuan black snake) from Received 3 June 2004; revision 3 August 2004; accepted 1 October 2004. Merauke, West Papua; and P. porphyriacus (red-bellied black snake) from 8 S Ramasamy et al. the Barossa Valley, South Australia. Pseudechis butleri (Butler’s snake) physiological solution was bubbled with carbogen (95% O2 and 5% CO2) from Yalgoo, Western Australia, and P. pailsii (eastern pygmy mulga) from and maintained at 34ЊC. Indirect twitches were evoked by stimulating the Mt Isa, Queensland, were milked by BGF. Freeze-dried venoms and stock motor nerve every 10 s with pulses of 0.2 msec duration at a supramaximal solutions of venoms were prepared in 0.1% bovine serum albumin (BSA) voltage7 using a Grass S88 stimulator (Grass Instrument Co., Quincy, MA, in 0.9% saline and stored at –20ЊC until required. Owing to the lack of USA). After a 30 min equilibration period, the nicotinic receptor antagonist availability of P. pailsii venom, this was not included in the antivenom D-tubocurarine (10 ␮mol/L) was added and the subsequent abolition of reversal studies. twitches confirmed the selective stimulation of the somatic nerve. Tissues were then washed thoroughly to re-establish twitches. Contractile responses Chick isolated biventer cervicis nerve–muscle to acetylcholine (ACh; 1 mmol/L for 30 s) and potassium chloride (KCl; 23 preparation 40 mmol/L for 30 s) were obtained in the absence of stimulation. Electrical stimulation was then recommenced and the preparations were Male chicks (4–10 days old) were killed by CO2 inhalation and exsanguin- allowed to equilibrate for a further 30 min period before commencement of ation and both biventer cervicis preparations were removed.22 Tissues were the experiment. Venom was left in contact with the preparations until mounted under 1 g resting tension in organ baths containing a physiological complete twitch blockade occurred or for a 60 min period. At the solution of the following composition (in mmol/L): NaCl 118.4; KCl 4.7; conclusion of the experiment, contractile responses to ACh and KCl were NaHCO3 2.5; KH2PO4 1.2; MgSO2 1.2; CaCl2 2.5; glucose 11.1. The then obtained, as described previously. Fig. 1 Effect of prior administration of black snake antivenom (5 U/mL; n = 3–5; ᮀ) or tiger snake antivenom (5 U/mL; n = 3–4; ᭝) on Pseudechis venom (10 µg/mL; n = 4–7)-induced inhibition of indirect twitches in the chick biventer cervicis nerve–muscle preparation. (᭺), Pseudechis venom alone. (a) Pseudechis australis; (b) Pseudechis butleri; (c) Pseudechis colletti; (d) Pseudechis guttatus; (e) Pseudechis pailsii; (f) Pseudechis papuanus; (g) Pseudechis porphyri- acus. *P < 0.05 compared with vehicle control (one-way ANOVA followed by a Bonferroni post hoc test). Neurotoxic effects of Pseudechis venoms 9 Black or tiger snake antivenom (5 U/mL) was added 10 min prior to P. porphyriacus Ϸ P. pailsii Ϸ P. guttatus > P. papuanus venom or at t90 (i.e. the time-point at which the original twitch height was Ն P. colletti > P. australis (t90 values are given in Table 1). All reduced by 90%) of individual tissues and kept in contact with the tissue for venoms produced a significant decrease in the contractile response the remainder of the experiment. of the chick biventer nerve–muscle preparation to ACh (1 mmol/L; Ethical approval for all animal experiments was obtained from the Monash University Animal Ethics Committee. n = 4–7; P < 0.05, one-way ANOVA; Table 2), with venoms from some species significantly affecting contractile responses to KCl (40 mmol/L; n = 4–7; P < 0.05, one-way ANOVA; Table 3). Drugs Black snake antivenom (5 U/mL), tiger snake antivenom Acetylcholine chloride and D-tubocurarine chloride were purchased from (5 U/mL) or vehicle (BSA) alone had no significant effect on either Sigma (St Louis, MO, USA). Stock solutions were made up in distilled the indirect twitches (data not shown, n = 4) or the contractile water. Black snake antivenom, raised against P. australis venom, and tiger responses of the chick biventer cervicis nerve–muscle preparation snake antivenom, raised against Notechis scutatus venom, were purchased to exogenous ACh (1 mmol/L; Table 2) or KCl (40 mmol/L; from CSL Ltd (Melbourne, Victoria, Australia). Table 3). Statistics Prior administration of the antivenom All statistics were performed using Sigmastat v.2.0 (Systat Software, Black snake antivenom (5 U/mL) prevented the inhibition of Richmond, CA, USA). Responses are expressed as the meanϮSEM. Data ␮ were analysed using one-way ANOVA, followed by a post hoc test (Bonfer- indirect twitches induced by all Pseudechis venoms (10 g/mL; roni). In all cases, statistical significance is indicated by P < 0.05. Fig. 1a–g) and at least partially restored contractile responses to exogenous ACh (1 mmol/L) to an extent that was indistinguishable from that of the antivenom control (n = 3–5; P < 0.05, one-way RESULTS ANOVA; Table 2). With the exception of P. porphyriacus venom, Effect of Pseudechis venoms on indirect twitches pretreatment with black snake antivenom prevented the venom- induced reduction of contractile responses to KCl (40 mmol/L; All Pseudechis venoms (10 ␮g/mL) produced a significant n = 3–5; P < 0.05, one-way ANOVA; Table 3).