JOURNAL OF VIROLOGY Volume 84 March 2010 No. 6 SPOTLIGHT Articles of Significant Interest Selected from This Issue by 2635 the Editors STRUCTURE AND ASSEMBLY The Capsid Proteins of Aleutian Mink Disease Virus Fang Cheng, Aaron Yun Chen, 2687–2696 Activate Caspases and Are Specifically Cleaved during Sonja M. Best, Marshall E. Bloom, Infection David Pintel, and Jianming Qiu Interaction Domains of the UL16 and UL21 Tegument Amy L. Harper, David G. Meckes, 2963–2971 Proteins of Herpes Simplex Virus Jr., Jacob A. Marsh, Michael D. Ward, Pei-Chun Yeh, Nicholas L. Baird, Carol B. Wilson, O. John Semmes, and John W. Wills Serotype-Specific Structural Differences in the Protease- Sumana Chandramouli, Jeremiah S. 3059–3067 Cofactor Complexes of the Dengue Virus Family Joseph, Sophie Daudenarde, Jovylyn Gatchalian, Cromwell Cornillez-Ty, and Peter Kuhn GENOME REPLICATION AND REGULATION OF VIRAL GENE EXPRESSION The DR1 and DR6 First Exons of Human Herpesvirus 6A Ronen Borenstein, Haim 2648–2656 Are Not Required for Virus Replication in Culture and Are Zeigerman, and Niza Frenkel Deleted in Virus Stocks That Replicate Well in T-Cell Lines The Hepatitis B Virus X Protein Modulates Hepatocyte Tricia L. Gearhart and Michael J. 2675–2686 Proliferation Pathways To Stimulate Viral Replication Bouchard Identification of an ICP27-Responsive Element in the Lenka Sedlackova, Keith D. Perkins, 2707–2718 Coding Region of a Herpes Simplex Virus Type 1 Late Gene Julia Meyer, Anna K. Strain, Oksana Goldman, and Stephen A. Rice In Vitro Synthesis of Sindbis Virus Genomic and Mei-Ling Li, Hongtao Wang, and 2732–2739 Subgenomic RNAs: Influence of nsP4 Mutations and Victor Stollar Nucleoside Triphosphate Concentrations The p38 Signaling Pathway Upregulates Expression of the Pegah Johansson, Ann Jansson, Ulla 2787–2797 Epstein-Barr Virus LMP1 Oncogene Ru¨etschi, and Lars Rymo The Foamy Virus Genome Remains Unintegrated in the Yung-Tsun Lo, Tao Tian, Peter E. 2832–2842 Nuclei of G1/S Phase-Arrested Cells, and Integrase Is Nadeau, Jeonghae Park, and Ayalew Critical for Preintegration Complex Transport into the Mergia Nucleus Poly(A) at the 3؅ End of Positive-Strand RNA and VPg- Benjamin P. Steil, Brian J. Kempf, 2843–2858 Linked Poly(U) at the 5؅ End of Negative-Strand RNA Are and David J. Barton Reciprocal Templates during Replication of Poliovirus RNA Autoexcision of Bacterial Artificial Chromosome Facilitated Fuchun Zhou, Qiuhua Li, Scott W. 2871–2880 by Terminal Repeat-Mediated Homologous Recombination: Wong, and Shou-Jiang Gao a Novel Approach for Generating Traceless Genetic Mutants of Herpesviruses Proteasome Subunits Relocalize during Human Karen Tran, Jeffrey A. Mahr, and 3079–3093 Cytomegalovirus Infection, and Proteasome Activity Is Deborah H. Spector Necessary for Efficient Viral Gene Transcription Continued on following page Continued from preceding page GENETIC DIVERSITY AND EVOLUTION Evidence for Multiple Recent Host Species Shifts among the James K. Jancovich, Michel 2636–2647 Ranaviruses (Family Iridoviridae) Bremont, Jeffrey W. Touchman, and Bertram L. Jacobs ؊/؊ ␥ ؊/؊ Evolution of the HIV-1 env Gene in the Rag2 C William L. Ince, Liguo Zhang, Qi 2740–2752 Humanized Mouse Model Jiang, Kathryn Arrildt, Lishan Su, and Ronald Swanstrom Ecoepidemiology and Complete Genome Comparison of Susanna K. P. Lau, Kenneth S. M. 2808–2819 Different Strains of Severe Acute Respiratory Syndrome- Li, Yi Huang, Chung-Tong Shek, Related Rhinolophus Bat Coronavirus in China Reveal Bats Herman Tse, Ming Wang, Garnet as a Reservoir for Acute, Self-Limiting Infection That Allows K. Y. Choi, Huifang Xu, Carol S. F. Recombination Events Lam, Rongtong Guo, Kwok-Hung Chan, Bo-Jian Zheng, Patrick C. Y. Woo, and Kwok-Yung Yuen VIRUS-CELL INTERACTIONS Pseudotyping Incompatibility between HIV-1 and Gibbon Tiffany M. Lucas, Terri D. Lyddon, 2666–2674 Ape Leukemia Virus Env Is Modulated by Vpu Paula M. Cannon, and Marc C. Johnson Epigenetic Regulation of Kaposi’s Sarcoma-Associated Fang Lu, William Stedman, Malik 2697–2706 Herpesvirus Latency by Virus-Encoded MicroRNAs That Yousef, Rolf Renne, and Paul M. Target Rta and the Cellular Rbl2-DNMT Pathway Lieberman Involvement of Ceramide in the Propagation of Japanese Hideki Tani, Mai Shiokawa, Yuuki 2798–2807 Encephalitis Virus Kaname, Hiroto Kambara, Yoshio Mori, Takayuki Abe, Kohji Moriishi, and Yoshiharu Matsuura Functional Analysis of RNA Structures Present at the 3؅ Dalan Bailey, Ioannis Karakasiliotis, 2859–2870 Extremity of the Murine Norovirus Genome: the Variable Surender Vashist, Liliane Man Wah Polypyrimidine Tract Plays a Role in Viral Virulence Chung, Jivan Reese, Nora McFadden, Alicia Benson, Felix Yarovinsky, Peter Simmonds, and Ian Goodfellow A Single Amino Acid Substitution in the Capsid of Foot- Miguel A. Martín-Acebes, Vero´nica 2902–2912 and-Mouth Disease Virus Can Increase Acid Lability and Rinco´n, Rosario Armas-Portela, Confer Resistance to Acid-Dependent Uncoating Inhibition Mauricio G. Mateu, and Francisco Sobrino GLTSCR2/PICT-1, a Putative Tumor Suppressor Gene Inna Kalt, Tatyana Borodianskiy- 2935–2945 Product, Induces the Nucleolar Targeting of the Kaposi’s Shteinberg, Adi Schachor, and Sarcoma-Associated Herpesvirus KS-Bcl-2 Protein Ronit Sarid Biochemical and Structural Characterization of Cathepsin Chantelle L. Hood, Jonathan 2972–2982 L-Processed Ebola Virus Glycoprotein: Implications for Abraham, Jeffrey C. Boyington, Viral Entry and Immunogenicity Kwanyee Leung, Peter D. Kwong, and Gary J. Nabel Sustainability of Virulence in a Phage-Bacterial Ecosystem Silja Heilmann, Kim Sneppen, and 3016–3022 Sandeep Krishna MicroRNA Expression and Virulence in Pandemic Influenza Yu Li, Eric Y. Chan, Jiangning Li, 3023–3032 Virus-Infected Mice Chester Ni, Xinxia Peng, Elizabeth Rosenzweig, Terrence M. Tumpey, and Michael G. Katze N-Linked Glycan at Residue 523 of Human Parainfluenza Vasiliy P. Mishin, Makiko 3094–3100 Virus Type 3 Hemagglutinin-Neuraminidase Masks a Watanabe, Garry Taylor, John Second Receptor-Binding Site DeVincenzo, Michael Bose, Allen Portner, and Irina V. Alymova Identification of the CD163 Protein Domains Involved in Hanne Van Gorp, Wander Van 3101–3105 Infection of the Porcine Reproductive and Respiratory Breedam, Jan Van Doorsselaere, Syndrome Virus Peter L. Delputte, and Hans J. Nauwynck Continued on following page Continued from preceding page Rapid Dissociation of HIV-1 from Cultured Cells Severely Emily J. Platt, Susan L. Kozak, 3106–3110 Limits Infectivity Assays, Causes the Inactivation Ascribed James P. Durnin, Thomas Hope, to Entry Inhibitors, and Masks the Inherently High Level of and David Kabat Infectivity of Virions Effect of Mutations in the Human Immunodeficiency Virus Abdul A. Waheed, Sherimay D. 3121–3126 Type 1 Protease on Cleavage of the gp41 Cytoplasmic Tail Ablan, Raymond C. Sowder, James D. Roser, Carl P. Schaffner, Elena Chertova, and Eric O. Freed TRANSFORMATION AND ONCOGENESIS Adenovirus Type 5 E1A and E6 Proteins of Low-Risk Jessica Komorek, Mohan 2719–2731 Cutaneous Beta-Human Papillomaviruses Suppress Cell Kuppuswamy, T. Subramanian, S. Transformation through Interaction with FOXK1/K2 Vijayalingam, Elena Lomonosova, Transcription Factors Ling-jun Zhao, Joe S. Mymryk, Kimberly Schmitt, and G. Chinnadurai Epstein-Barr Virus-Encoded Bcl-2 Homologue Functions as Ami Watanabe, Seiji Maruo, Taku 2893–2901 a Survival Factor in Wp-Restricted Burkitt Lymphoma Cell Ito, Miho Ito, Koichi Ricardo Line P3HR-1 Katsumura, and Kenzo Takada VACCINES AND ANTIVIRAL AGENTS Characterization of a Single-Cycle Rabies Virus-Based Emily A. Gomme, Elizabeth J. Faul, 2820–2831 Vaccine Vector Phyllis Flomenberg, James P. McGettigan, and Matthias J. Schnell 1a/1b Subtype Profiling of Nonnucleoside Polymerase O. Nyanguile, B. Devogelaere, L. 2923–2934 Inhibitors of Hepatitis C Virus Vijgen, W. Van den Broeck, F. Pauwels, M. D. Cummings, H. L. De Bondt, A. M. Vos, J. M. Berke, O. Lenz, G. Vandercruyssen, K. Vermeiren, W. Mostmans, P. Dehertogh, F. Delouvroy, S. Vendeville, K. VanDyck, K. Dockx, E. Cleiren, P. Raboisson, K. A. Simmen, and G. C. Fanning Relationship between Antibody 2F5 Neutralization of HIV-1 Gilad Ofek, Krisha McKee, 2955–2962 and Hydrophobicity of Its Heavy Chain Third Yongping Yang, Zhi-Yong Yang, Complementarity-Determining Region Jeff Skinner, F. Javier Guenaga, Richard Wyatt, Michael B. Zwick, Gary J. Nabel, John R. Mascola, and Peter D. Kwong Efficacy of Multivalent Adenovirus-Based Vaccine against D. R. Casimiro, K. Cox, A. Tang, 2996–3003 Simian Immunodeficiency Virus Challenge K. J. Sykes, M. Feng, F. Wang, A. Bett, W. A. Schleif, X. Liang, J. Flynn, T. W. Tobery, K. Wilson, A. Finnefrock, L. Huang, S. Vitelli, J. Lin, D. Patel, M.-E. Davies, G. J. Heidecker, D. C. Freed, S. Dubey, D. H. O’Connor, D. I. Watkins, Z.-Q. Zhang, and J. W. Shiver PATHOGENESIS AND IMMUNITY No Increase in Hepatitis B Virus (HBV)-Specific CD8؉ T Megan Crane, Sunee Sirivichayakul, 2657–2665 Cells in Patients with HIV-1–HBV Coinfections following J. Judy Chang, Anchalee HBV-Active Highly Active Antiretroviral Therapy Avihingsanon, Sasiwimol Ubolyam, Supranee Buranapraditkun, Pattarawat Thantiworasit, Fiona Wightman, Stephen Locarnini, Gail Matthews, Gregory J. Dore, Kiat Ruxrungtham, and Sharon R. Lewin Continued on following page Continued from preceding page Canine Distemper Viruses Expressing a Hemagglutinin Bevan Sawatsky and Veronika von 2753–2761 without N-Glycans Lose Virulence but Retain Messling Immunosuppression Inability of Plasmacytoid Dendritic Cells To Directly Lyse Jihed Chehimi,