International Symposium on Problems of Listeriosis Ficha Técnica LIVRO DE ACTAS DO CONGRESSO: ISOPOL XVII Intrenational Symposium on Problems of Listeriosis Editor: Universidade Católica Portuguesa – Escola Superior de Biotecnologia Coordenação e Revisão: Paula Teixeira Design e Composição Gráfica: Kai Sprecher / Lynn Salt Impressão: Orgal Impressores Depósito Legal: Tiragem: 500 exemplares WELCOME TO ISOPOL XVII On behalf of the Organising Committee, I am pleased to welcome you to ISOPOL XVII (International Symposium On Problems Of Listeriosis) which this year is organized in Porto by the Universidade Católica Portuguesa – Escola Superior de Biotecnologia. The ISOPOL meetings provide a unique opportunity for interdisciplinary discussions concerning various aspects of listeriosis including, but not being limited to, food safety and clinical aspects. Since the first edition of this symposium in Giessen, Germany (1957), much has been discovered and many more questions have been posed. For this reason the underlying mo- tives for bringing together the evolving ISOPOL community are still very strong in 2010. More than 350 delegates from the clinical, veterinary, and public health areas as well as the food industry, will be present, representing ca. 45 coun- tries. This diversity will certainly provide for a very stimulating and plural atmosphere which is sure to promote rich discussions and exchanges of ideas. On this occasion, we would like to thank to the members of the Scientific Committee for their time and effort in maintaining the high scientific level of ISOPOL XVII. We are also thankful to our sponsors, which, in many different ways, have greatly contributed to the success of the organization of this event. For the first time ever, ISOPOL will take place in Portugal, namely in Porto – the country’s second city and the capital of the north. It is easy to see why Porto was designated a World Heritage Site by UN- ESCO in 1996. It is a living city, full of monuments and signs of its rich his- tory. It is best met in person; take the opportunity to stay awhile, to meet the people, to enjoy the charms of this city of contrasts – you may find the need to return again to enjoy it all! Paula Cristina Maia Teixeira TABLE OF CONTENTS KEY NOTE SPEECH Listeria monocytogenes: a multifaceted model 21 Cossart, P. AREA //A // Biology of Listeria monocytogenes PLENARY LECTURES A / PL/ 01 The pangenome of Listera spp. 22 Chakraborty, T. A / PL/ 02 Ecology of L. monocytogenes and Listeria spp. in natural 22 and food associated environments Wiedmann, M. ORAL PRESENTATIONS A / O/ 01 The cold shock associated proteins (Csps) promote tolerance 33 of different environmental stresses and host cell invasion of Listeria monocytogenes Tasara, T., Klumpp, J., Loessner, M. J. and Stephan, R. A / O/ 02 Different levels of flagellin detected during growth of 33 Listeria monocytogenes strains at low temperature Cabrita, P., Batista, S., Moes, S., Jenö, P., Trigo, M. J., Boavida Ferreira, R. and Brito, L. A / O/ 03 Phenotypic and corresponding transcriptomic responses of 34 Listeria monocytogenes strains in the presence of unprotonated organic acids Lee Chang, K. J., Pinfold, T., Koshy, A. and Bowman, J. P. A / O/ 04 Internalin profiling, multilocus sequence typing and 34 virulence assesments suggest evolutionary history of the Listeria monocytogenes-Listeria innocua clade Chen, J. and Fang, W. A / O/ 05 The SOS response of Listeria monocytogenes is involved 35 in stress resistance, mutagenesis, and biofilm formation van der Veen, S. and Abee, T. A / O/ 06 Clonal diversity of Listeria monocytogenes, a worldwide perspective 35 Chenal-Francisque, V., Lopez, J., Cantinelli, T., Caro, V., Tran, C., Leclerq, A., Lecuit, M. and Brisse, S. A / O/ 07 Life without a cell wall: Listeria monocytogenes L-form cells 36 feature a unique mode of division Briers, Y., Dell’Era, S., Schuppler, M. and Loessner, M. J. A / O/ 08 Pangenomic analysis of Listeria monocytogenes 36 Deng, X., Phillippy, A. M., Li, Z., Salzberg, S. L., Tortorello, M. L. and Zhang, W. A / O/ 09 Evidence for an antiporter-independent glutamate decarboxylase 37 (GAD) system in Listeria monocytogenes: Influence of growth media on GAD system activity Karatzas, K.-A., Brennan, O., Heavin, S. and O’Byrne, C. P. A / O/ 10 Role of Listeria monocytogenes tyrosine phosphatases 37 in conferring listeriophage resistance Paz, R.-N., Eugster, M. R., Zeiman, E., Loessner, M. J. and Calendar, R. A / O/ 11 Thiolomics – the thiol: disulfide redox metabolism of Listeria monocytogenes 38 Ondrusch, N., Gopal, S., Fuss, A., Hagen, N., Stoll, R., Aharonowitz, Y. and Kreft, J. A / O/ 12 RNA-structures acting at a distance 38 Johansson, J. A / O/ 13 Deep RNA sequencing of Listeria monocytogenes revealsoverlapping 39 and extensive stationary phase and sigma B-dependent transcriptomes, including multiple highly transcribed noncoding RNAs Oliver, H. F., Orsi, R. H., Ponnala, L., Keich, U., Wang, W., Sun, Q., Cartinhour, S., Filiatrault, M. J., Wiedmann, M. and Boor, K. J. POSTER PRESENTATIONS A / P/ 00 CompartmentalizationofIFN-gammaandIL-6receptorssignalling 62 in Listeria monocytogenes phagosomes: immune vesicles Ramos-Vivas, J., Carrasco-Marin, E., Madrazo-Toca, F., Fernandez-Prieto, L., Rodriguez-Del Rio, E., Carranza-Cereceda, C. and Alvarez-Dominguez, C. A / P/ 01 Antimicrobial susceptibility among Listeria monocytogenes isolates 63 from non human sources in France over a ten year period Granier, S. A., Moubarek, C., Colaneri, C., Roussel, S., Courvalin, P. and Brisabois, A. A / P/ 02 Five homologous small RNAs are involved in the response 63 of Listeria monocytogenes to cell wall acting antibiotics Kiil Nielsen, P. and Kallipolitis, B. A / P/ 03 Phenotypic analysis of selected listerial secretion mutants 64 Halbedel, S., Galander, S. and Flieger, A. A / P/ 04 Distribution of serotypes and pulsotypes of L. monocytogenes 64 in pig farms (France 2008) Boscher, E. A / P/ 05 Comparative phylogenomics of Listeria monocytogenes reveals 65 an adaptation profile Silveira Nalério, E., Padilha Silva, W., Stabler, R. and Wren, B. W. A / P/ 06 Serotyping and PFGE patterns of Listeria monocytogenes isolated 65 from poultry meat Vasantrao Kurkure, N., Kalorey, D. R., Rodrigues, J., Gunjal, P.1 and Barbuddhe, S. B. A / P/ 07 Genotypic characterization of Listeria monocytogenes isolated 66 from fresh leafy vegetables Warke, S., Kalorey, D. R., Umap, S., Sonegaonkar, A., Patil, V., Kurkure, N V. and Barbuddhe, S. B. A / P/ 08 Comprehensive appraisal of the exoproteome of Listeria monocytogenes 66 by genomic and proteomic analyses Desvaux, M., Dumas, E., Chafsey, I., Chambon, C. and Hébraud, M. A / P/ 09 Investigating differences in lineages of Listeria monocytogenes 67 using comparative genomics McIlwham, S., Farber, J. and Pagotto, F. A / P/ 10 Autolysis in Listeria monocytogenes – a proteomic approach 67 Pinto, E., Marques, N., Andrew, P. W. and Faleiro, M. L. A / P/ 11 Role of flhA, cheR and motA in growth of Listeria monocytogenes 68 at low temperature Mattila, M., Lindström, M., Somervuo, P. and Korkeala, H. A / P/ 12 Theeffectofaceticacid(atpH5.5)orbenzoicacid(atneutralpH)onlipid 68 composition and fluidity of Listeria monocytogenes membrane Ioannis, D., Anita, B., Eleni, S. and Mastronicolis, S. A / P/ 13 Elucidation of the responses to weak acids in the human pathogen 69 Listeria monocytogenes using gene microarrays O’Byrne, C., Heavin, S. and Morrissey, J. A / P/ 14 The immunogenic surface protein IspC acts as an N-Acetylglucosaminidase 69 in Listeria monocytogenes serotype 4b Ronholm, J. A / P/ 15 Listeria monocytogenes EGD chitinolytic activity is regulated 70 by carbohydrates but also by the virulence regulatory gene, PrfA Halberg Larsen, M., Leisner, J. J. and Ingmer, H. A / P/ 16 Infectious dose curves for guinea pigs challenged with a Listeria 70 monocytogenes epidemic clone strain and a strain carrying a naturally-occurring virulence-attenuating mutation in inlA show a significant shift in median infectious dose Nightingale, K., Van Stelten, A., Simpson, J. M., Chen, Y., Scott, V. N., Ross, W. H., Whiting, R. C. and Wiedmann, M. A / P/ 17 MudPITbasedproteomicanalysisofalkalineadapted,environmentally 71 persistent Listeria monocytogenes strains Nilsson, R. E., Ross, T. and Bowman, J. P. A / P/ 18 RpoN,thealternativesigmafactor,isassociatedwiththegrowthphase 71 transition and pathogenesis in Listeria monocytogenes Okada, Y., Suzuki, H., Monden, S., Igimi, S. and Okada, N. A / P/ 19 Cellular lipid fatty acid pattern differences between reference 72 and ice-cream isolate of Listeria monocytogenes as response to cold stress Anita, B., Ioannis, D. and Mastronicolis, S. A / P/ 20 Role of the dihydroxyacetone metabolism in the resistance 72 of Listeria innocua to pediocin Milohanic, E. A / P/ 21 Glucose transport system in Listeria monocytogenes 73 and their impact on virulence gene expression Moussan Ake, F. A / P/ 22 Antimicrobial susceptibilities of Listeria monocytogenes isolatedinJapan 73 Monden, S., Okutani, A., Suzuki, H., Asakura, H., Nakama, A., Igimi, S., Okada, Y. and Maruyama, T. A / P/ 23 Influence of sub-lethal concentrations of disinfectants 74 on Listeria monocytogenes adhesion and invasion in Caco-2 cells Gaedt Kastbjerg, V., Halberg Larsen, M., Ingmer, H. and Gram, L. A / P/ 24 The SOS response in Listeria monocytogenes –astresssurvivalmechanism 74 Kiil Nielsen, P., Zahle Andersen, A. and Haahr Kallipolitis, B. A / P/ 25 Acid shock triggers heavy metal detoxification in Listeria monocytogenes 75 Müller, S., Neuhaus,