Downloaded from orbit.dtu.dk on: Sep 24, 2021 Yeast perfume factory: metabolic engineering of Saccharomyces cerevisiae for plant isoprenoid biosynthesis Gionata, S.; Knuf, C.; Siavash, P.; Chen, Y.; Maury, Jerome; Schalk, M.; Daviet, L.; Nielsen, Jens; Siewers, V. Published in: F E B S Journal Link to article, DOI: 10.1111/j.1742-4658.2010.07680.x Publication date: 2010 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Gionata, S., Knuf, C., Siavash, P., Chen, Y., Maury, J., Schalk, M., Daviet, L., Nielsen, J., & Siewers, V. (2010). Yeast perfume factory: metabolic engineering of Saccharomyces cerevisiae for plant isoprenoid biosynthesis. F E B S Journal, 277(s1), 234. 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A1 – Ageing Abstracts A1 – Ageing cognitive function of a 7-day, intraperitoneal administration of A1.01 saffron was examined in healthy adult and aged mice by step through test. Results showed that saffron-treated mice exhibited Abstract withdrawn significant improvement in learning and memory. Experiments in whole brain homogenates revealed that saffron administration resulted in significantly lower brain lipid peroxidation (malondial- A1.02 dehyde, 44–63%) and higher antioxidant parameters (glutathione, BDNF Mediated Angiogenesis Potential is ascorbic acid, total antioxidant power). Salt- and detergent-solu- ble AChE activity was significantly decreased only in adult mice. Decreased Associated with Aging Thus, the significant cognitive enhancement conferred by saffron D. Cai, L. Cao, S. Chen, D. Li, X. Shen, X Zheng and X. Liu administration in adult and aged mice, is closely related to the Ji Nan University, Key Laboratory for Regenerative Medicine, antioxidant reinforcement; AChE inhibition (in adult mice) plays Minstry of Education, Guangzhou, China also a minor role. Studying further the antioxidant potential, the The mechanism of age-related decrease of angiogenic potential in effect(s) of saffron and crocetin (main crocin metabolite), were myocardium is still unclear. Cardiac microvascular endothelial examined against H2O2-induced toxicity in SH-SY5Y and cells (CMECs) play a key role in cardiac angiogenesis. In this HEK293 cells. Cell viability and scavenging of free radicals after study, using the CMECs which are isolated from young and old co-treatment with H2O2 (250–750 lM) and the tested compounds hearts, we found that the migration and proliferation capacity of (1–250 lg/ml saffron, 1–125 lM crocetin) were determined with old CMECs were diminished. BDNF was able to increase the MTT and DCF assays. Results showed that saffron and crocetin migration and proliferation of CMECs no matter in young and provide strong protection in rescuing cell viability and repressing old CMECs, however, the effects in old CMECs was less potent ROS production in the SH-SY5Y cells; moderate effects in compared with the young CMECs. In vivo study showed that HEK293 cells. Considering, thus, earlier metabolic studies, croce- delivery of BDNF in young heart in ischemic situation was able tin appears to be responsible for the in vivo effects. to increase the vessel numbers in both infarct and border zone significantly, but not in young heart in non-ischemic situation. The microarray results showed that 84 genes were up-regulated, while 81 genes were down-regulated upon BDNF treatment. The functional annotations of genes are cell migration, blood vessel A1.04 morphogenesis, angiogenesis, regulation of proliferation, cell Protective effects of triphlorethol-A against cycle regulation, etc, which have been shown the strong potential formaldehyde-induced oxidative damage and effects in migration, proliferation and angiogenesis. The results apoptosis: role of mitochondria-mediated of present study revealed that BDNF-TrkB pathway play an caspase-dependent pathway important role in angiogenesis of myocardium. Although CMECs R. Zhang1, K. A. Kang1, M. J. Piao1, K. C. Kim1, J. Y. Choi2, express BDNF consistently, however, BDNF might not initiate J. Choi3, J. Park4 and J. W. Hyun1 the angiogenesis in heart individually in vivo. BDNF-mediated 1School of Medicine, Jeju National University, Jejusi, Republic of angiogenic potential might depend on the cross talk with focal Korea, 2Department of Pharmacology, School of Medicine, Ewha micro-environment. Importantly, senescence of CMECs was able Womans University, Seoul, Republic of Korea, 3Faculty of to impair the BDNF-mediated migration and proliferation capac- Environmental Engineering, University of Seoul, Seoul, Republic of ity. It might contribute to age-related decrease of angiogenic Korea, 4Division of Hematology and Oncology, Department of potential in myocardium and poor regenerative capacity seen in Internal Medicine, Gachon University of Medicine Science, Gil aged heart. Hospital, Incheon, Republic of Korea The toxicity of formaldehyde (HCHO) has been attributed to its ability to form adducts with DNA and proteins. Triphlor- ethol-A, derived from Ecklonia cava, was reported to exert a A1.03 cytoprotective effect against oxidative stress damage via an anti- Memory enhancing effects of saffron in adult oxidant mechanism. The aim of this study was to examine the & aged mice are correlated with the mechanisms underlying triphlorethol-A ability to protect Chi- antioxidant protection: In vitro and in vivo nese hamster lung fibroblast (V79-4) cells against HCHO- studies induced damage. Triphlorethol-A significantly decreased the HCHO-induced intracellular reactive oxygen species (ROS) pro- M. Papandreou1, M. Tsachaki2, S. Efthimiopoulos3, duction. Triphlorethol-A prevented increased cell damage P. Cordopatis4, F. Lamari4 and M. Margarity1 induced by HCHO via inhibition of mitochondria-mediated cas- 1University of Patras, Biology, Lab. Human & Animal Physiology, pase-dependent apoptosis pathway. Triphlorethol-A diminished Patras, Greece, 2University of Athens, Biology, Lab. Animal & HCHO-induced mitochondrial dysfunction including loss of Human Physiology, Athens, Greece, 3University of Athens, mitochondrial membrane action potential (Y) and adenosine tri- Biology, Animal & Human Physiology, Athens, Greece, 4University phosphate (ATP) depletion. Furthermore, the anti-apoptotic of Patras, Pharmacy, Lab. Pharmacognosy & Chemistry of effect of triphlorethol-A was exerted through inhibition of c-Jun Natural Products, Patras, Greece NH2-terminal kinase (JNK) which was enhanced by HCHO. Oxidative stress is implicated in senescence and age-related Our data indicate that triphlorethol-A exerts a cytoprotective pathologies, with memory deficits as the commonest manifesta- effect in V79-4 cells against HCHO-induced oxidative stress by tions. Herbal ingredients are sought to forestall/reverse those def- inhibiting the mitochondria-mediated caspase-dependent apopto- icits as dietary components/or supplements. The effect on tic pathway. FEBS Journal 277 (Suppl. 1) 37–271 (2010) ª 2010 The Authors Journal compilation ª 2010 Federation of European Biochemical Societies 37 Abstracts A1 – Ageing A1.05 A1.07 Antioxidant effect of Jeju water containing Analysis of ageing and stress resistance in vanadium component natural clones K. A. Kang1, R. Zhang1, M. J. Piao1, K. C. Kim1, C. M. Lim1, H. Nilsson Sko¨ ld1, C. Owesson1, B. Carney Almroth2, A. D. Kim2 and J. W. Hyun1 M. Asplund3, C. Woods4, J. Bishop4, M. Sko¨ ld5 and S. Wing6 1School of Medicine, Jeju National University, Jejusi, Republic of 1University of Gothenburg/Marine Ecology, Fiskeba¨ckskil, Sweden, Korea, 2Department of Marine Life Science, Jeju National 2University of Gothenburg/Zoology, Gothenburg, Sweden, University, Jejusi, Republic of Korea 3University of Gothenburg/Zoology, Fiskeba¨ckskil, Sweden, 4Marine Biological Association, Plymouth, UK, 5Department of The aim of this study was to examine the antioxidant effect of Fisheries, Lysekil, Sweden, 6Otago University, Dunedin, New Jeju water containing vanadium component (20–25 ppb). Cells Zealand were incubated for 10 passages in media containing deionized dis- tilled water (DW group) and Jeju water (JW group). DW and In organisms that propagate by agametic cloning, the parental JW groups did not show to scavenge 1,1-diphenyl-2-pic- body is the reproductive unit and fitness increases with size of rylhydrazyl radical. Electron spin resonance spectrometer data the colony, why such metazoans have despite lack of experimen- showed that JW group significantly scavenged superoxide radicals tal data been considered potentially immortal. However, most induced by Fenton reaction (H2O2+FeSO4), and hydroxyl radi- clonal organisms derive evolutionary from sexually reproducing cals induced by xanthine/xanthin oxidase system as compared to ancestors, why they may have inherited ageing.