AGROFOR International Journal PUBLISHER University of East Sarajevo, Faculty of Agriculture Vuka Karadzica 30, 71123 East Sarajevo, Bosnia and Herzegovina Telephone/fax: +387 57 340 401; +387 57 342 701 Web: www.agrofor.rs.ba; Email: [email protected] EDITOR-IN-CHIEF Vesna MILIC (BOSNIA AND HERZEGOVINA) MANAGING EDITORS Dusan KOVACEVIC (SERBIA); Sinisa BERJAN (BOSNIA AND HERZEGOVINA); Noureddin DRIOUECH (ITALY) EDITORIAL BOARD Dieter TRAUTZ (GERMANY); Hamid El BILALI (ITALY); William H. MEYERS (USA); Milic CUROVIC (MONTENEGRO); Tatjana PANDUREVIC (BOSNIA AND HERZEGOVINA); Alexey LUKIN (RUSSIA); Machito MIHARA (JAPAN); Abdulvahed KHALEDI DARVISHAN (IRAN); Viorel ION (ROMANIA); Novo PRZULJ (BOSNIA AND HERZEGOVINA); Steve QUARRIE (UNITED KINGDOM); Hiromu OKAZAWA (JAPAN); Snezana JANKOVIC (SERBIA); Naser SABAGHNIA (IRAN); Sasa ORLOVIC (SERBIA); Sanja RADONJIC (MONTENEGRO); Junaid Alam MEMON (PAKISTAN); Vlado KOVACEVIC (CROATIA); Marko GUTALJ (BOSNIA AND HERZEGOVINA); Dragan MILATOVIC (SERBIA); Pandi ZDRULI (ITALY); Zoran JOVOVIC (MONTENEGRO); Vojislav TRKULJA (BOSNIA AND HERZEGOVINA); Zoran NJEGOVAN (SERBIA); Adriano CIANI (ITALY); Aleksandra DESPOTOVIC (MONTENEGRO); Igor DJURDJIC (BOSNIA AND HERZEGOVINA); Stefan BOJIC (BOSNIA AND HERZEGOVINA); Julijana TRIFKOVIC (BOSNIA AND HERZEGOVINA) TECHNICAL EDITORS Milan JUGOVIC (BOSNIA AND HERZEGOVINA) Luka FILIPOVIC (MONTENEGRO) Frequency: 3 times per year Number of copies: 300 ISSN 2490-3434 (Printed) ISSN 2490-3442 (Online) CONTENT PATHOLOGY OF TESTES CELLS IN WHITE MICE AFTER IMPACT OF EPRINOMECTIN Anastasia SOGRINA, Vera BEREZHKO, Lyudmila NAPISANOVA, Tatiana PROHOROVA ..................................................................................................................... 6 HABITAT TYPES OF EUROPEAN IMPORTANCE IN THE AREA OF WETLANDS GROMIZELJ (BOSNIA AND HERZEGOVINA) Sladjana PETRONIC, Natasa BRATIC, Tanja JAKISIC, Vesna TUNGUZ ................ 10 SOCIAL NETWORK SITES UTILIZED IN AGRICULTURAL EXTENSION SERVICES IN KINGDOM OF SAUDI ARABIA Bander M. ALSAGHAN, Ahmed M. DIAB, Abdullah S. ALHOTAN ....................... 19 COMPARATIVE CHARACTERISTICS OF PHOTOSYNTHETIC ACTIVITY OF NECTARINE CULTIVARS AND FORMS WITH DIFFERENT COLORED LEAF PLATE Iuliia IVASHCHENKO, Yuriy IVASHCHENKO, Evgeny SHOFERISTOV, Sergei TSIUPKA ........................................................................................................................... 28 SEED GERMINATION RATES OF DIFFERENT COOL SEASON LEGUMES Elpiniki SKOUFOGIANNI, Dimitrios BARTZIALIS, Kyriakos D. GIANNOULIS, Evangelia KANDRI, Nicholaos G. DANALATOS ........................................................ 35 PRE-SOWING SEED INOCULATION IN THE BIRDSFOOT TREFOIL SEED PRODUCTION Vladeta STEVOVIĆ, Đorđe LAZAREVIĆ, Dalibor TOMIĆ, Dušica DELIĆ, Dragan ĐUROVIĆ .......................................................................................................................... 41 THE CONTENT OF ESSENTIAL ELEMENTS IN THE FLOWERS AND FRUITS OF CHAENOMELES (Chaenomeles Lindl. ) Larisa KOMAR-TYOMNAYA, Elena DUNAEVSKAYA .......................................... 48 EXPLORING HOUSEHOLD FOOD WASTE ISSUE IN ALGERIA Samir Ali AROUS, Roberto CAPONE, Philipp DEBS, Yasmina HADDADI, Hamid EL BILALI, Francesco BOTTALICO, , Mohamed HAMIDOUCHE ........................... 55 SANITARY CONDITION OF PICEA ABIES (L. ) KARST. YOUNG FOREST STANDS DEPENDING ON THE SPATIAL SPECIFICITY Jelena RUBA, Olga MIEZITE, Imants LIEPA ............................................................... 68 GENETIC VARIABILITY OF THE CEREAL (POACEAE) GERMPLASM COLLECTION MONITORED BY PROTEIN AND MOLECULAR MARKERS Miodrag DIMITRIJEVIĆ, Sofija PETROVIĆ, Borislav BANJAC, Goran BARAĆ, Aleksandra YURIEVNA DRAGOVIĆ, Alexander MIHAILOVICH KUDRYAVTSEV, Desimir KNEŽEVIĆ ....................................................................... 75 3 INFLUENCE OF AGROCHEMICAL REHABILITATION ON THE HEAVY METAL MIGRATION TO THE WATER Yuri MAZHAYSKIY, Olga CHERNIKOVA, Alexey KARPOV, Otilija MISECKAITE ................................................................................................................... 83 ADAPTIVE CAPACITY OF SOME LAVENDER AND LAVANDIN CULTIVARSIN VITRO AND IN SITU Oksana GREBENNIKOVA, Anfisa PALIY, Valentina BRAILKO, Olga MITROFANOVA, Valery RABOTYAGOV, Irina ZHDANOVA, Irina MITROFANOVA .............................................................................................................. 91 MODELLING RESERVOIR SEDIMENTATION AT BIN EL OUIDANE DAM, MOROCCO El Mouatassime SABRI, Ahmed BOUKDIR, Rachid El MASLOUHI, Mustapha MABROUKI, Abdellah EL MAHBOUL, Vivien Romaric EKOUELE MBAKI, Abdelhamid ZITOUNI, WissalBAITE, Zhour ECHAKRAOUI ................................... 99 CONDITIONS OF AGRICULTURAL LAND PRICES DEVELOPMENT IN POLAND Renata MARKS-BIELSKA ............................................................................................ 109 THE EFFECT OF APPLIED CROSSBREEDING ON INCREASING OF MILK PRODUCTION IN SHEEP Pascal CONSTANTIN, Atanasiu TRAIAN, Nechifor IONICĂ, , Costică CRISTIAN ........................................................................................................................................... 119 CEREALS SEED LIFECYCLE Sergej V. GONCHAROV ............................................................................................... 125 LEVEL OF LARVAL ATACK ON MAIZE ROOTS AS A CONSEQUENCE OF ARTIFICIAL INFESTATIONWITH WESTERN CORN ROOTWORM EGGS Snežana TANASKOVIĆ, Branka POPOVIĆ, Sonja GVOZDENAC, Zsolt KARPÁTI, Csengele BÓGNAR, Matthias ERB ............................................................................... 132 GERMINATION CHARACTERISTICS OF TEDERA (BITUMINARIA BITUMINOSA VAR. BITUMINOSA) Marta BARBERÁ, María P. FLORES, José I. R. CASTANON, Erik DIAZ-AVILA, Myriam R. VENTURA ................................................................................................... 139 SEQUENCE ANALYSIS OF INSECTICIDE RESISTANCE AND DETOXIFICATION RELATED GENES IN Spodoptera littoralis(LEPIDOPTERA: NOCTUIDAE) Nurper GUZ, Aslı DAĞERİ ........................................................................................... 144 SEEKING AN OPTIMAL CLASSIFICATION SYSTEM FOR THE COMMERCIAL CATEGORIES OF OLIVE OILS Carla MARANO-MARCOLINI, Francisco J. TORRES-RUIZ, Esther LÓPEZ- ZAFRA ............................................................................................................................. 154 4 INDEX OF AUTHORS ................................................................................................ 166 INSTRUCTIONS FOR AUTHORS ........................................................................... 167 5 AGROFOR International Journal, Vol. 2, Issue No. 1, 2017 Original Scientific Paper 10.7251/AGRENG1701006S UDC 616:57.085 PATHOLOGY OF TESTES CELLS IN WHITE MICE AFTER IMPACT OF EPRINOMECTIN Anastasia SOGRINA1, Vera BEREZHKO1, Lyudmila NAPISANOVA1, Tatiana PROHOROVA2 1All Russian Institute of Parasitology, Moscow, Russia ²Perm State Agricultural Academy, Perm, Russia *Corresponding author: sograv@yandex. ru ABSTRACT Avermectines are new class of antibiotics with acaricidal and anthelmintic activity. New medicine containing macrocyclic lactones is Eprimec. One milliliter of Eprimec contains 10mg of eprinomectin as active substance. The aim of our study was to determine the extent of karyopatic and cytotoxic effects of that preparation to cell division process in testes of white mice. The materials for the study were the non-linear white mice – males 18 - 22 g. Experimental group of mice received a dose of 200 micrograms of active compound per 1 kg of weight or 10 mg / ml of eprinomectin by single subcutaneous injection. The control group of animals remained intact. Each group consisted of 8 mice. The animals were slaughtered at 12 hours after drug administration. Testes of mice were isolated, smears – imprints were prepared that are fixed by May - Grunewald and stained with azure - eosin by Romanovsky. Glass slides with testes cells were viewed under a microscope and implemented to count the number of dividing cells, take into account the shape, size and color of the nuclei. Analysis of the frequency of occurrence of a karyopatic disorders and cytotoxic effects of cells were performed separately and lying sprawled cells with counting at least 1000 cells in each glass slide (Lazareva et al, 2016). Analyzing the data, we concluded that on the basis of eprinomectin formulations significantly reduce the activity of cell division, but it is toxic and cause serious karyopatic changes in the cells of the testes nonlinear white mice, indicating that the negative effects on the reproductive system of males. Keywords: antiparasitic medications, Еprinomectin, karyopatic effects. INTRODUCTION Works of plenty of scientists evidence the kariopatic effect of chemical substances and antigens (Аlov, 1972; Tolbert et al., 1992; Morgan, 2007; Lazareva et al., 2016). Among the latest antiparasitic drugs a special place has avermectins are new class of antibiotics possessing insecticidal, acaricidal and antihelminthic effect. This pharmacological group of substances widely used for the treatment and prevention of parasitic diseases of agricultural, small domestic and exotic animals. Ivomec and its copy – Baymak, Sevmek, Bisection, Pandeks, Ivermectin, Everton, 6 AGROFOR International Journal, Vol. 2, Issue No. 1, 2017 Novomec are most popular for veterinarians. The popularity of products containing macrocyclic lactones of the following generations increases, in particular drug Eprimec. Eprimec