SAN TA C RUZ BI OTEC HNOL OG Y, INC . MS4A3 (M-13): sc-109328 BACKGROUND PRODUCT MS4A (membrane-spanning 4-domain family, subfamily A) is a large family Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide of proteins that includes at least 26 members in mouse and humans. Flanked and 0.1% gelatin. by amino- and carboxyl- cytoplasmic regions, MS4A family members contain Blocking peptide available for competition studies, sc-109328 P, (100 µg four highly conserved transmembrane domains. CD20, the most well-known peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). MS4A family member, is a B-cell-specific molecule that functions as a calci - um-permeable cation channel and is known to accelerate the G to G pro - 0 1 APPLICATIONS gression induced by IGF-1. MS4A3 (membrane-spanning 4-domains, subfam - ily A, member 3), also known as HTM4 (hematopoietic-specific transmem - MS4A3 (M-13) is recommended for detection of MS4A3 of mouse and rat brane protein 4) or CD20L, is a 214 amino acid protein that acts as a modu - origin by Western Blotting (starting dilution 1:200, dilution range 1:100- 1:1000), immunofluorescence (starting dilution 1:50, dilution range 1:50- lator for the G 1-S cell cycle transition. A multi-pass membrane protein, MS4A3 localizes to the endomembrane system and is also found in the cytoplasm. 1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30- MS4A3 is expressed in hematopoietic cells and tissues and interacts with 1:3000); non cross-reactive with other MS4A family members. CDKN3. Suitable for use as control antibody for MS4A3 siRNA (m): sc-149647, MS4A3 shRNA Plasmid (m): sc-149647-SH and MS4A3 shRNA (m) Lentiviral REFERENCES Particles: sc-149647-V. 1. Adra, C.N., et al. 1994. Cloning of the cDNA for a hematopoietic cell-spe - Molecular Weight of MS4A3 isoforms: 23/18 kDa. cific protein related to CD20 and the β subunit of the high-affinity IgE receptor: evidence for a family of proteins with four membrane-spanning RECOMMENDED SECONDARY REAGENTS regions. Proc. Natl. Acad. Sci. USA 91: 10178-10182. To ensure optimal results, the following support (secondary) reagents are 2. Ishibashi, K., et al. 2001. Identification of a new multigene four-transmem - recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 brane family (MS4A) related to CD20, HTm4 and β subunit of the high- affin - (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey ity IgE receptor. Gene 264: 87-93. anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 3. Liang, Y., et al. 2001. Identification of a CD20-, Fc εRI β-, and HTm4-related gene family: sixteen new MS4A family members expressed in human and sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- mouse. Genomics 72: 119-127. rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) 4. Liang, Y., et al. 2001. Structural organization of the human MS4A gene with UltraCruz™ Mounting Medium: sc-24941. cluster on Chromosome 11q12. Immunogenetics 53: 357-368. 5. Donato, J.L., et al. 2002. Human HTm4 is a hematopoietic cell cycle regu - RESEARCH USE lator. J. Clin. Invest. 109: 51-58. For research use only, not for use in diagnostic procedures. 6. Gray, C.H., et al. 2003. Getting in the ring: proline-directed substrate speci - ficity in the cell cycle proteins Cdc14 and CDK2-cyclinA3. Cell Cycle 2: PROTOCOLS 500-502. See our web site at www.scbt.com or our catalog for detailed protocols and 7. Chinami, M., et al. 2005. Binding of HTm4 to cyclin-dependent kinase (Cdk)- support products. associated phosphatase (KAP).Cdk2.cyclin A complex enhances the phos - phatase activity of KAP, dissociates cyclin A, and facilitates KAP dephos - phorylation of Cdk2. J. Biol. Chem. 280: 17235-17242. ZCHROMOSOMAL LOCATION Genetic locus: Ms4a3 (mouse) mapping to 19 A. SOURCE MS4A3 (M-13) is an affinity purified goat polyclonal antibody raised against a peptide mapping within a C-terminal cytoplasmic domain of MS4A3 of mouse origin. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.