Comparison of antinociceptive activity of topical formulations containing extracts of plants of the genus Jatropha against the venom of the scorpion Tityus stigmurus Beatriz K Da C Batista1; Jacinthia B Xavier-Santos1; Júlia G R Passos1; João F O Silva1; Arnóbio A Silva-Júnior1; Matheus De F Fernandes-Pedrosa1 1 Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, RN, Brazil [email protected] INTRODUCTION ➔ Scorpion envenomings are common in tropical and subtropical regions [1]. In the Brazilian Northeast region the most significant species is Tityus stigmurus, causing symptoms as pain (94,4% of cases) and edema [2], [3], [4]. ➔ The treatments performed against envenoming are supportive therapy and Figure 1: Tityus stigmurus serotherapy, in more severe cases [5]. ➔ Plants of the genus Jatropha presented anti-inflammatory activity and analgesic potential [6] [7], being able to act in the local effects caused by scorpion venom. ➔ This study seeks to evaluate the antinociceptive activity of J. molissima and J. gossypiifolia regarding its against the Figure 2: Jatropha mollissima venom of the scorpion T. stigmurus (TsV). (Pohl) Baill. Figure 3: Jatropha gossypiifolia Passos, 2019. Félix-Silva et al., 2014. METHODS Rafael Godeiro and Monte Alegre, Rio Grande do Norte, Northeast region, Brazil Botanical Maceration 48h Remake 48h Ethanol 70% Ethanol 70% identification 1:10 (w/v, plant:water) 1:10 (w/v, plant:water) Semi-solid formulation Lyophilization Filtration (Xavier-Santos et al.. 2018) Thin Layer Chromatography Brazilian Biodiversity Authorization and System (SISBIO) n° 41490-4 Brazilian Genetic Heritage Management Council (CGEN) n° 010844/2013-9 Ethics Committee on Animal Use from Federal University of Rio Grande do Norte n°065/2019 Rota-Rod test Treatment Antinociceptive activity Hot plate test ● Jatropha molissima gel 1” ● Jatropha gossypiifolia gel and TsV/PBS ● Anti-scorpion serum ● Gel EXPECTED RESULTS ➔ If there are favorable results, the semi-solid formulation becomes an alternative for individuals who have difficult access to antiscorpion serum, reducing hospital costs and possible adverse reactions caused by the serum. REFERENCES [1] FUKUHARA, Y. D. M. et al. The kinin system in the envenomation caused by the Tityus serrulatus scorpion sting. Toxicology and Applied Pharmacology, v. 196, n. 3, p. 390-395, 2004. [2] FREIRE, M. C. L. C. et al. Molecular basis of Tityus stigmurus alpha toxin and potassium channel kV1.2 interactions. Journal of Molecular Graphics and Modelling, v. 87, n., p. 197-203, 2019. [3] ALMEIDA, D. D. et al. Molecular approaches for structural characterization of a new potassium channel blocker from Tityus stigmurus venom: cDNA cloning, homology modeling, dynamic simulations and docking. Biochemical and Biophysical Research Communications, v. 430, n. 1, p. 113-118, 2013. [4] LIRA-DA-SILVA, R. M.; AMORIM, A. M. D.; BRAZIL, T. K. Envenenamento por Tityus stigmurus (Scorpiones; Buthidae) no Estado da Bahia, Brasil. Revista da Sociedade Brasileira de Medicina Tropical, v. 33, n., p. 239-245, 2000. [5] DE OLIVEIRA YAMASHITA, F. et al. Mangaba (Hancornia speciosa Gomes) fruit juice decreases acute pulmonary edema induced by Tityus serrulatus venom: Potential application for auxiliary treatment of scorpion stings. Toxicon, v. 179, n., p. 42-52, 2020. [6] UCHE, D. F.; APRIOKU, J. The Phytochemical Constituents, Analgesic and Anti-inflammatory effects of methanol extract of Jatropha curcas leaves in Mice and Wister albino rats. ed.: 2010. p. [7] SILVA, C. R. et al. The antinociceptive and anti-inflammatory effects of the crude extract of Jatropha isabellei in a rat gout model. Journal of Ethnopharmacology, v. 145, n. 1, p. 205-213, 2013..