Catalog # Aliquot Size P34-911-05 3 x 5 nmol P34-911-20 3 x 20 nmol P34-911-50 3 x 50 nmol PTPN7 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # P34-911 Lot # Z2085-26 Specificity Formulation PTPN7 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PTPN7 expression. shipped at room temperature. Product Description Reconstitution Protocol PTPN7 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PTPN7 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_002832. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot LC-PTP, LPTP, HEPTP, PTPNI, BPTP-4. 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PTPN1 (PTP1B), Active P28-20G below -20oC. After resuspension, siRNA stock solutions ≥2 PTPN11 (SHP2), Active P38-21G µM can undergo up to 50 freeze-thaw cycles without PTPN12 (PTP-PEST), Active P39-21G significant degradation. For long-term storage, it is PTPN13 (FAP-1), Active P40-21G recommended that the siRNA is stored at -70oC. For most PTPN2 (TC-PTP), Active P29-20G favorable performance, avoid repeated handling and PTPN6 (SHP1), Active P33-20G multiple freeze/thaw cycles. PTPN7 (LC-PTP), Active P34-20G Anti-PTPN11 (SHP2) P38-63CR Scientific Background Anti-PTPN11 (SHP2) P38-63BR PTPN7 gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells (1). The noncatalytic N- terminus of this PTP can interact with MAP kinases and PTPN7 siRNA Set I siRNA duplexes targeted against three exon regions negatively regulates ERK2 and p38 MAP-kinases activity (2). The PTPN7 was shown to be involved in the regulation Catalog # P34-911 of T cell antigen receptor (TCR) signaling, which was Lot # Z2085-26 thought to function through dephosphorylating the Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol molecules related to MAP kinase pathway (3). Format Lyophilized powder Stability 1yr at -70oC from date of shipment References Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is 1. Adachi, M. et al: Protein-tyrosine phosphatase recommended that the lyophilized expression in pre-B cell NALM-6. Cancer Res. 52: 737- and resuspended siRNAs are stored at 740, 1992. or below -20oC. After resuspension, 2. Pettiford, S M. et al: The MAP-kinase ERK2 is a specific siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles substrate of the protein tyrosine phosphatase HePTP. without significant degradation. For Oncogene. 2000 Feb 17;19(7):858-69. long-term storage, it is recommended 3. Oh-hora, M. et al: Direct .suppression of TCR- that the siRNA is stored at -70oC. For most favorable performance, avoid mediated activation of extracellular signal-regulated repeated handling and multiple kinase by leukocyte protein tyrosine phosphatase, a freeze/thaw cycles. tyrosine-specific phosphatase. J Immunol. 1999 Aug 1;163(3):1282-8. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .