OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AR51491PU-S Human HERC5 (681-1024, His-tag) - Purified Alternate names: CEB1, CEBP1, Cyclin-E-binding protein 1, E3 ISG15--protein ligase HERC5, HECT domain and RCC1-like domain-containing protein 5 Quantity: 0.1 mg Concentration: 1.0 mg/ml (determined by Bradford assay) Background: HERC5 is a member of the HERC family of ubiquitin ligases. It is a protein with a HECT domain and five RCC1 repeats. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. HERC5 gene lies in a cluster of HERC family genes on chromosome 4. It has been shown to exhibit antiviral activity towards HIV-1, influenza A virus and human papillomavirus. Uniprot ID: Q9UII4 NCBI: NP_057407 GeneID: 51191 Species: Human Source: E. coli Format: State: Liquid purified protein Purity: >90% by SDS - PAGE Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol Description: Recombinant human HERC5 protein, fused to His-tag at N-terminus, was expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSFDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG Molecular weight: 43 kDa (367aa) Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: Woods MW, Kelly JN, et al. (2011). Retrovirology. 8:95. Tang Y, Zhong G, et al. (2010). J Immunol. 184(10):5777-90. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20131001 1 / 2 AR51491PU-S: Human HERC5 (681-1024, His-tag) - Purified Pictures: For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20131001 2 / 2 Powered by TCPDF (www.tcpdf.org).