Leader in Biomolecular Solutions for Life Science MYH2 Rabbit pAb Catalog No.: A15292 1 Publications Basic Information Background Catalog No. Myosins are actin-based motor proteins that function in the generation of mechanical A15292 force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a Observed MW member of the class II or conventional myosin heavy chains, and functions in skeletal 250KDa muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple Calculated MW alternatively spliced variants, encoding the same protein, have been identified. 79kDa/223kDa Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:200 - 1:2000 Gene ID Swiss Prot 4620 Q9UKX2 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MYH2 (NP_001093582.1). Synonyms MYH2;IBM3;MYH2A;MYHSA2;MYHas8;MYPOP;MyHC-2A;MyHC-IIa;myosin-2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of Rat skeletal muscle, using MYH2 antibody (A15292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. Western blot analysis of extracts of Mouse skeletal muscle, using MYH2 antibody (A15292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. Immunofluorescence analysis of mouse skeletal muscle using MYH2 Polyclonal Antibody (A15292) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..