CAMKK2 (Human) Recombinant Protein

CAMKK2 (Human) Recombinant Protein

CAMKK2 (Human) Recombinant Gene Summary: The product of this gene belongs to Protein the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Catalog Number: P6478 This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by Regulation Status: For research use only (RUO) phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct Product Description: Human CAMKK2 (NP_006540.3, isoforms have been identified for this gene. Additional 1 a.a. - 588 a.a.) full length recombinant protein with splice variants have been described but their full-length GST-tag at N-terminal using baculovirus expression nature has not been determined. The identified isoforms system. exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases. [provided Host: Viruses by RefSeq] Applications: Func (See our web site product page for detailed applications information) Protocols: See our web site at http://www.abnova.com/support/protocols.asp or product page for detailed protocols Form: Liquid Preparation Method: Baculovirus expression system. Purification: Glutathione sepharose chromatography. Purity: 72% Activity: The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate, Mg (or Mn)/ATP, and Ca/Calmodulin. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: CAMKK substrate, ATP: 500 uM. Storage Buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5 Storage Instruction: Stored at -80°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 10645 Gene Symbol: CAMKK2 Gene Alias: CAMKK, CAMKKB, KIAA0787, MGC15254 Page 1/1 Powered by TCPDF (www.tcpdf.org).

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