The 17th Annual Kansas-IDeA Network of Biomedical Research Excellence Symposium January 19-20, 2019 Overland Park Sheraton Overland Park, KS This program was made possible by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences (NIGMS) of the National Institutes of Health (NIH) under grant number P20 GM103418. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Overland Park Sheraton Floor Plan LOCATION OF EVENTS • Registration: Lobby Level/ Leatherwood Foyer • Friday Night Events: Cottonwood Ballroom and Foyer • Breakfast: Cottonwood Ballroom and Foyer • General Session: Leatherwood Ballroom • Breaks: Leatherwood Foyer • Lunch: Cottonwood Ballroom and Foyer • Poster Sessions/Reception: Cottonwood Ballroom • Dinner: Leatherwood Ballroom • Boxed Lunches: Leatherwood Foyer K-INBRE 2019 Symposium Table of Contents Program Schedule……………………………………………………………………………………………………………… Page 2 Platform Presentation Abstracts……………………………………………………………………………………..... Page 5 Poster Presentations…………………………………………………………………………………………………………. Page 7 Poster Presentation Abstracts…………………………………………………………………………………………… Page 17 Symposium Participants……………………………………………………………………………………………………. Page 60 Notes…………………………………………………………………………………………………………………………………. Page 74 Poster Presentations Saturday, January 19, 2019 Poster Session I (3:30-4:30 PM) Abstracts Numbered 1-40 Poster Session II (4:30-5:30 PM) Abstracts Numbered 41-80 Poster Session III (5:30-6:30 PM) Abstracts Numbered 81-103 Sunday, January 20, 2019 Poster Session IV (9:40-10:25 AM) Abstracts Numbered 104-136 Poster Session V (10:25-11:10 AM) Abstracts Numbered 137-173 See Alphabetical list for monitor assignment. Abstract# = Monitor # Please be near your monitor during your assigned session until the judges have visited. Feel free to visit other boards during the alternate sessions. IMPORTANT: Please ensure that all publications resulting from INBRE funds are in compliance with the NIH Public Access Policy. Future awards from NIH will be delayed until evidence of compliance has been demonstrated. For more information on the Public Access policy, please visit this link: http://publicaccess.nih.gov/policy.htm When K-INBRE funds have supported your research, please remember to acknowledge this support by including the grant number P20 GM103418, regardless of the time period between receipt of funding and the publication or presentation. 1 K-INBRE 2019 Symposium Program Schedule Overland Park Sheraton Overland Park, KS Friday, January 18, 2019 3:00 PM Early Registration Foyer Open Poster viewing Cottonwood Ballroom 4:30 PM Early Registration Closes 6:00 PM Friday Night Event and Dinner 8:00 PM Poster Viewing, Friday Night Event and Dinner Ends Saturday, January 19, 2019 7:30 AM Breakfast Buffet Cottonwood Ballroom/Foyer Registration Foyer 8:30 AM General Session Leatherwood Ballroom Doug Wright, Ph.D., K-INBRE Principal Investigator, University of Kansas Medical Center Opening Remarks 9:15 AM Gregory Finnigan, Ph.D. Assistant Professor, Kansas State University Moderator: Keynote and Regional Scientist Presentations 9:20 AM David Scott McVey, D.V.M., Ph.D., D.A.C.V.M., Director, Center for Grain and Animal Health Research, United States Department of Agriculture Title: Vaccines: There and Back Again 9:55 AM C. Ron Yu, Ph.D., Investigator, Stowers Institute for Medical Research Title: What is critical in the critical period of olfactory system development? 10:25 AM Break Foyer University Photos 10:30 AM Haskell Indian Nations University Photo 10:35 AM Fort Hays State University Photo 10:40 AM Kansas State University Photo 10:45 AM Pittsburg State University Photo 10:50 AM University of Kansas Medical Center Photo 10:55 AM General Session Leatherwood Ballroom E. Matthew Morris, Ph.D., Research Assistant Professor, University of Kansas Medical Center Moderator: Regional Scientist and Trainee Presentations 11:00 AM Adam Smith, Ph.D., Assistant Professor, University of Kansas, Lawrence Title: Wired for social relationships 11:30 AM Joshua Miller, Kansas State University, Manhattan, KS Title: Novel Pilot Study Reveals that Heat Therapy Increases Muscle Mitochondrial Quality Control and Respiratory Efficiency in Healthy Human Subjects 2 Saturday, January 19, 2019 11:50 AM Cayla Moore, Langston University, Langston, OK Title: Hydrocortisone Decreases the Proliferation Rate and Increases Antibiotic Resistance of Streptococcus Pneumoniae 12:10 PM Lunch Cottonwood Ballroom/Foyer 1:15 PM General Session Leatherwood Ballroom Jianzhong Yu, Ph.D., Assistant Professor, Kansas State University Moderator: Regional Scientist and Trainee Presentations 1:20 PM Prachee Avasthi, Ph.D., Assistant Professor, University of Kansas Medical Center Title: A tale of two actins: significant functional overlap of divergent actin isoforms in the unicellular green alga Chlamydomonas reinhardtii 1:50 PM Vaughn Craddock, University of Kansas, Lawrence, KS Title: Genetic Adaptations Conferring Quorum-Sensing Dependent Antibiotic Resistance in Evolved Strains of Pseudomonas aeruginosa 2:10 PM Annabelle Dillon, Kansas State University, Manhattan, KS Title: Impact of reduced liver PGC1a during development on adult diet-induced weight gain 2:30 PM Erina Kutilek, Wichita State University, Wichita, KS Title: The Role of Myopalladin in Cardiac Muscle Functions and Disease 2:50 PM General Session Concludes Foyer University Photos 2:55 PM Langston University Photo 3:00 PM Emporia State University Photo 3:05 PM University of Kansas, Lawrence Photo 3:10 PM Washburn University Photo 3:15 PM Wichita State University Photo 3:25 PM Poster Judge Meeting Maple Room 3:30 PM Reception/Poster Session I Cottonwood Ballroom 4:30 PM Reception/Poster Session II Cottonwood Ballroom 5:30 PM Reception/Poster Session III Cottonwood Ballroom 6:30 PM Poster Session Ends Dinner Leatherwood Ballroom 7:00 PM Award Presentations Leatherwood Ballroom John Stanford, Ph.D., K-INBRE Associate Director, University of Kansas Medical Center Doug Wright, Ph.D., K-INBRE Principal Investigator, University of Kansas Medical Center Closing Remarks 3 Sunday, January 20, 2019 7:00 AM Breakfast Buffet Foyer 8:00 AM General Session Leatherwood Ballroom John Stanford, Ph.D., Associate Director, University of Kansas Medical Center Opening Remarks 8:05 AM Stephanie Shames, Ph.D., Assistant Professor, Kansas State University Moderator: Regional Scientist and Trainee Presentation 8:10 AM Fawwaz Naeem, Kansas State University, Manhattan, KS Title: Maintenance of muscle proteostasis 8:30 AM Kendall Odle, Langston University, Langston, OK Title: Connecting Tumor Suppressor Genes to DNA Damage Response 8:50 AM Shwetha Ramachandran, University of Kansas Medical Center, Kansas City, KS Title: Defective ciliary signaling in initiation of renal cystic disease in Thm1 conditional knock-out mice 9:10 AM Jeffrey A. Thompson, Ph.D., Assistant Professor, University of Kansas Medical Center Title: A statistical approach to determining equivalent functional genomic differences across experiments 9:40 AM Poster Session IV Cottonwood Ballroom 10:25 AM Poster Session V Cottonwood Ballroom 11:10 AM Poster Session V ends 11:15 AM General Session Leatherwood Ballroom John Stanford, Ph.D., Associate Director, University of Kansas Medical Center Introduction of Speaker 11:20 AM James Beck, Ph.D., Assistant Professor, Wichita State University Title: RADseq techniques can be applied to DNAs derived from plant museum specimens 11:50 AM John Stanford, Ph.D., Associate Director, University of Kansas Medical Center Oral Presentation Awards 12:00 PM Doug Wright, Ph.D., K-INBRE Principal Investigator, University of Kansas Medical Center Closing Remarks 12:00 PM Boxed lunches available for pickup Foyer 12:00 PM Hotel Checkout 4 Platform Presentation Abstracts Saturday, January 19, 2019 Novel Pilot Study Reveals that Heat Therapy Increases Muscle Mitochondrial Quality Control and Respiratory Efficiency in Healthy Human Subjects Joshua Miller1, Alex Von Schulze1, Kelly Fuller1, Colin McCoin1, Clara Amat Fernandez1, John Thyfault1, and Paige Geiger1 1The University of Kansas City Medical Center – Department of Integrative and Molecular Physiology We have shown that heat therapy (HT) in rodents protects against high-fat diet induced skeletal muscle insulin resistance and impaired glucose homeostasis. However, it remains unknown whether a similar effect can be translated to humans. The purpose of this pilot study was to determine if HT via hot water immersion was well tolerated in humans and altered mitochondrial metabolism in skeletal muscle. Healthy male and female participants (n=5/sex) received one bout of HT to raise core temperature to 38.5°C for 1h. Muscle biopsies were taken one week prior to HT and 24h post HT to determine whether HT impacted mitochondrial respiratory capacity and mitophagy. HT was well tolerated in all subjects and resulted in increased heart rate and reduced systolic and diastolic blood pressure. HT did not significantly alter maximal mitochondrial respiratory capacity at basal, state3, state3S, or uncoupled states with either palmitoyl-carnitine + palmitoyl-CoA (palmitate) or pyruvate + glutamate + malate (PGM) as substrates. However, HT tended to improve respiratory efficiency as indicated by a reduced coupling control ratio for PGM. This improved efficiency and maintenance of mitochondrial respiratory capacity occurred despite HT-induced reductions in mitochondrial content as inferred by citrate