C146-E161B For LabSolutions LCMS LC/MS/MS Method Package for Veterinary Drugs LCMS-8045 Ready-to-Use Methods Provided Shimadzu provides method files, including pre-registered MRM parameters with optimized quantitative and reference ions, LC separation parameters, and retention times and peak identification parameters for each compound, as well as report templates for outputting quantitation results, as a package. If retention times are adjusted when the system is introduced, based on the HPLC configuration delivered, the analysis process can be started as soon as the specified columns, mobile phases, and standard samples are supplied. Methods Easily Customized Since LabSolutions Version 5.41 SP1 enables the addition or deletion of components to be analyzed, methods can be easily customized. In addition, the package includes a compound parameter list enables the creation of new tables that list only the components needed. HPLC Conditions Gradient Program Analytical column : Shim-pack HR-ODS (150 mmL. x 3.0 mmI.D., 3µm) Step Time (min) A (%) B (%) P/N 228-59908-95 0 0 99 1 Mobile phase A : 0.1 % Formic acid - water Mobile phase B : Acetonitrile 1 35 0 100 Flow rate : 0.4 mL/min 2 40 0 100 Injection volume : 10 µL 3 40.1 99 1 4 50 99 1 For LabSolutions LCMS LC/MS/MS Method Package for Veterinary Drugs List of Compounds No. Compound Name No. Compound Name No. Compound Name No. Compound Name 1 Levamisole 12 Sulfathiazole 23 Sulfadoxine 34 Melengestrol Acetate 2 Lincomycin 13 Trichlorfon (DEP) 24 Sulfamethoxazole 35 Temephos (Abate) 3 5-Propylsulfonyl-1H-benzimidazole-2-amine 14 Sulfapyridine 25 Prednisolone 36 Allethrin 4 Trimethoprim 15 Pyrimethamine 26 Ethopabate 37 Monensin 5 Thiabendazole 16 Tilmicosin 27 Sulfaquinoxaline 38 Florfenicol 6 Ormetoprim 17 Sulfamerazine 28 Sulfadimethoxine 39 2-Acetylamino-5-nitrothiazole 7 Sulfacetamide 18 Sulfadimidine 29 Dexamethasone 40 Clorsulon 8 Danofloxacin 19 Sulfamethoxypyridiazine 30 Emamectin B1a 41 Sulfanitran 9 Xylazine 20 Sulfamonomethoxine 31 beta-Trenbolone 42 Zeranol 10 Clenbuterol 21 Sulfachlorpyridazine 32 alpha-Trenbolone 11 Sulfadiazine 22 Tiamulin 33 Fenobucarb (BPMC) Smooth Analysis of Multiple Analytes with LabSolutions Insight When analyzing data for multiple components and analytes, everything from batch analysis to the creation of reports can be performed by LabSolutions Insight. LabSolutions Insight is a software program equipped with quantitative analysis and screening functions. In the analysis window, information is displayed for each target compound and set of measurement data, and both quantitative values and area values can be listed. This allows the user to select the easiest display method for checking the data in accordance with the workflow. In addition, by using the flagging function, only the results exceeding specified standard values are displayed, improving the analytical throughput of multiple components and analytes subject to the regulations. Remarks and Precautions 1. LabSolutions LCMS Ver. 5.41 or later is required. 2. Shimadzu makes no warranty regarding the accuracy of information included in the database or the usefulness of information obtained from using the database. 3. Be sure to perform tests using standard samples to confirm qualitative and quantitative information obtained with the given system. 4. To reliably identify registered substances using this database, measure samples using the instrument parameters specified in the method template files included in the product. © Shimadzu Corporation, 2017 First Edition: March 2011 .