Multi-Mode Microplate Reader Synergy™ HT Operator’s Manual Synergy™ HT Multi-Mode Microplate Reader Operator’s Guide November 2008 © 2008 Part Number 7091000 Revision I BioTek® Instruments, Inc. ii | Preface Notices BioTek® Instruments, Inc. Highland Park, P.O. Box 998 Winooski, Vermont 05404-0998 USA All Rights Reserved © 2008, BioTek® Instruments, Incorporated. No part of this publication may be reproduced, transcribed, or transmitted in any form, or by any means electronic or mechanical, including photocopying and recording, for any purpose other than the purchaser’s use without written permission of BioTek Instruments, Inc. Trademarks BioTek® is a registered trademark, and Synergy™ HT, Gen5™, KC4™, and BioStack™ are trademarks of BioTek Instruments, Inc. All other trademarks are the property of their respective holders. Restrictions and Liabilities Information in this document is subject to change and does not represent a commitment by BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers. BioTek Instruments Contents | iii Contents Notices ...........................................................................................ii Contact Information ....................................................................... vii Revision History ............................................................................ viii Document Conventions .................................................................. viii Intended Use Statement ................................................................ xiii Quality Control ............................................................................. xiii Warranty & Product Registration...................................................... xiii Warnings ..................................................................................... xiv Hazards and Precautions ................................................................ xiv Directives..................................................................................... xvi Electromagnetic Interference and Susceptibility ................................ xvii User Safety ................................................................................. xvii Safety Symbols............................................................................xviii Chapter 1: Introduction.......................................................................1 Synergy™ HT Multi-Mode Microplate Reader........................................ 2 Features ........................................................................................ 3 Package Contents............................................................................ 4 Optional Accessories ........................................................................ 5 Product Support & Service................................................................ 6 Technical Assistance Center (TAC) ................................................ 6 Returning Instruments for Service/Repair ...................................... 6 Contacting BioTek for Applications Support .................................... 6 Chapter 2: Installation ........................................................................7 1: Unpack and Inspect the Reader .................................................... 8 2: Remove the Shipping Panel ........................................................10 3: Remove the Microplate Carrier Shipping Screw ..............................12 4: Install the Fluorescence Lamp Assembly .......................................13 5: Select an Appropriate Location ....................................................14 6: Connect the Power Supply ..........................................................15 7: Unpack and Inspect the Dispense Module .....................................16 8: Install the Dispense Module ........................................................19 9: Connect the Host Computer ........................................................23 10: Install Software on the Host Computer .......................................24 11: Turn on the Reader..................................................................24 12: Establish Communication ..........................................................25 Using Gen5 ..............................................................................26 Using KC4 ................................................................................26 13: Run a System Test ..................................................................27 14: Test the Injector System ..........................................................28 Register with BioTek .......................................................................30 Synergy HT Operator’s Manual iv | Preface Operational/Performance Qualification...............................................30 Repackaging and Shipping Instructions .............................................31 Chapter 3: Getting Started ................................................................35 Key Components............................................................................36 Power Switch, Carrier Eject Button, Microplate Carrier ....................36 Lamp Assembly and Filter Wheel Access .......................................37 Excitation and Emission Filter Wheels ...........................................38 Installing the Time-Resolved Fluorescence Cartridge ......................41 Configuring the System for Luminescence Measurements................42 The External Dispense Module.....................................................43 Gen5 Software...............................................................................46 Viewing/Updating the Filter and Wavelengths Tables ......................46 Creating Protocols and Experiments .............................................47 Controlling the Dispense Module..................................................50 KC4 Software ................................................................................52 Viewing/Updating the Filter and Wavelengths Tables ......................52 Creating Protocols .....................................................................53 Reading Plates ..........................................................................55 Controlling the Dispense Module..................................................56 Recommendations for Achieving Optimum Performance .......................59 Chapter 4: Instrument Qualification..................................................61 Overview ......................................................................................62 IQ/OQ/PQ .....................................................................................62 Recommended Qualification Schedule ...............................................64 System Test ..................................................................................65 Description...............................................................................65 Procedure ................................................................................66 Absorbance Plate Test.....................................................................70 Description...............................................................................70 Test Plate Certificates ................................................................71 Setup: Gen5.............................................................................72 Procedure: Gen5 .......................................................................73 Setup: KC4 ..............................................................................74 Procedure: KC4.........................................................................74 Results & Troubleshooting Tips....................................................78 Absorbance Liquid Tests..................................................................80 Stock Solution Formulation ..............................................................81 Liquid Test 1.............................................................................82 Liquid Test 2.............................................................................83 Liquid Test 3.............................................................................86 Fluorescence Tests .........................................................................89 Required Materials.....................................................................90 Test Solutions...........................................................................91 BioTek Instruments Contents | v Procedure ................................................................................92 Results Analysis ........................................................................92 Troubleshooting ........................................................................93 Pipette Map ..............................................................................94 Gen5 Protocol Reading Parameters ..............................................95 KC4 Protocol Reading Parameters ................................................96 Fluorescence Tests Using Methylumbelliferone...............................98 Dispense Module Tests.................................................................. 102 Required Materials..................................................................