YTHDC1 (P561) Antibody -20ºC Store at Support: +1-978-867-2388 (U.S.) www.cellsignal.com/support Orders: 877-616-2355 (U.S.) [email protected] Entrez-Gene ID #91746 #87459 New 09/17 UniProt ID #Q96MU7 For Research Use Only. Not For Use In Diagnostic Procedures. Applications Species Cross-Reactivity* Molecular Wt. Source Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not W H, M, R, Mk, (Hm) 110 kDa Rabbit** Endogenous aliquot the antibody. *Species cross-reactivity is determined by western blot. ** Anti-rabbit secondary antibodies must be used to Background: YTH domain-containing protein 1 (YTHDC1) and detect this antibody. YTH domain-containing protein 2 (YTHDC2) both belong to a family of proteins that bind to RNA. YTHDC1 and YTHDC2 both kDa 293T RAW 264.7COS-7 C6 Recommended Antibody Dilutions: recognize and bind to N6-methyladenosine(m6A)-containing Western blotting 1:1000 200 RNAs; binding is mediated through the YTH domains (1-3). For product specific protocols and a complete listing m6A is a modification that is present at internal sites of mRNAs 140 of recommended companion products please see the and some non-coding RNAs and plays a role in regulating product web page at www.cellsignal.com mRNA splicing, processing, and stability. YTHDC1, also known YTHDC1 100 as splicing factor YT521, regulates alternative splicing by func- Background References: tioning as a key regulator of exon-inclusion or exon-skipping. 80 (1) Xu, C. et al. (2015) J Biol Chem 290, 24902-13. YTHDC1 promotes exon-inclusion by recruiting pre-mRNA splicing factor SRSF3 to regions containing m6A, while repress- (2) Xiao, W. et al. (2016) Mol Cell 61, 507-19. ing exon-skipping by blocking SRSF10 binding to these same 60 (3) Xu, C. et al. (2014) Nat Chem Biol 10, 927-9. regions (2). Increased expression of YTHDC1 promotes malig- (4) Zhang, B. et al. (2015) Tumour Biol 37, 15543-15549. nant endometrial carcinoma (EC) through alternative splicing of 50 vascular endothelial growth factor A (VEGF-A), resulting in an (5) Hsu, P.J. et al. (2017) Cell Res 27, 1115-1127. increase in VEGF-165 isoform and increased EC cell invasion 40 (4). YTHDC2 functions to enhance the translation efficiency of target mRNAs and may play a role in spermatogenesis (5). Western blot analysis of extracts from various cell lines using Specificity/Sensitivity: YTHDC1 (P561) Antibody recognizes YTHDC1 (P561) Antibody. endogenous levels of total YTHDC1 protein. Source/Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide cor- responding to residues surrounding Pro561 of human YTHDC1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Tween is a registered trademark of ICI Americas, Inc. Thank you for your recent purchase. If you would like to provide a review visit cellsignal.com/comments. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. www.cellsignal.com © 2017 Cell Signaling Technology, Inc. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Applications: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide Species Cross-Reactivity: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—Horse All—all species expected Species enclosed in parentheses are predicted to react based on 100% homology..