SENP6, SUMO1/sentrin specific protease 6 polyclonal antibody biquitin-like molecules (UBLs), such as SUMO1 are or Research Use Only. Not for Ustructurally related to ubiquitin and can be ligated to tar- FDiagnostic or Therapeutic Use. get proteins in a similar manner as ubiquitin. However, co- Purchase does not include or carry valent attachment of UBLs does not result in degradation of any right to resell or transfer this product either as a stand-alone the modified proteins. SUMO1 modification is implicated in product or as a component of another the targeting of RANGAP1 to the nuclear pore complex, as product. Any use of this product other well as in stabilization of I-kappa-B-alpha from degradation than the permitted use without the by the 26S proteasome. Like ubiquitin, UBLs are synthe- express written authorization of Allele sized as precursor proteins, with 1 or more amino acids fol- Biotech is strictly prohibited lowing the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precur- sors need to be removed by UBL-specific proteases prior to their conjugation to target proteins. SUMO1/sentrin specific Website: www.allelebiotech.com protease 6 (SENP6) is a cysteine protease containing the Call: 1-800-991-RNAi/858-587-6645 conserved histidine, aspartic acid, and cysteine residues of (Pacific Time: 9:00AM~5:00PM) the catalytic triad and the invariant glutamine residue that Email: [email protected] helps form the oxyanion hole. SENP6 is exclusively local- ized to the cytoplasm of mammalian cells and shows a tight substrate specificity for SUMO1. The highest expression of SENP6 can be found in reproductive organs, namely testis, Box 1 | Basic Info ovary, and prostate but it is also expressed in colon and pe- ripheral blood leukocytes. Little or no SENP6 is detected in Cat. No. ABP-PAB-10339 brain, liver, lung, kidney, pancreas, spleen, thymus, heart, Animal ID RB0663-0664 and skeletal muscle. Host Rabbit Reactivity Human Format Purified Buffers Accession number NM_015571 Purified rabbit polyclonal antibody supplied in PBS with Amount 100µg 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH Alternative Name(s): buffers and neutralized immediately after elution then fol- SSP1, KIAA0797, SENP6, Sentrin-specific lowed by dialysis against PBS. protease 6, SUSP1 Immunogen KLH conjugated synthetic peptide comprised of amino ac- ids 1096 - 1113 [CEAPLGEGTEQYVNSISD] of the human SUMO1/sentrin specific protease 6 (SENP6) protein. References: 1. Muller S, Hoege C, Pyrowolakis G, Jentsch S: SUMO, ubiquitin’s mysterious cousin. Nat. Rev. Application Mol. Cell. Biol. 2(3): 202-10 (2001); review. Tested by peptide-specific ELISA (1:1,000). WB (1:100 2. Hochstrasser M: Evolution and function of ~1:500), IHC (1:50 ~1:100) ubiquitin-like protein-conjugation systems. Nat. Cell. Biol. 2(11): E206 (2000). 3. Kahyo T, Nishida T, Yasuda H: Involvement Storage of PIAS1 in the sumoylation of tumor suppressor p53. Mol. Cell 8(3): 713-718 (2001). Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw 4. Yeh ET, Gong L, Kamitani T: Ubiquitin-like proteins: new wines in new bottles. Gene 248 cycles. (1-2): 1-14 (2000). Allele Biotech-Introducing Cost Effectiveness to Research.