M.Sc. Biotechnology (Two year programme with practicals) Scheme of instruction and examination (For students admitted from 2017-2018 and onwards) Hrs of instruct- Scheme of Examination tions / Subject Name of Paper / week Part Code Component T P CE CIA Total Credit of Exam Duration Duration First Semester I 17MBTC01 Biochemical Concepts for Biotechnology 4 – 3 40 60 100 3 I 17MBTC02 Cell Biology and Microbiology 4 – 3 40 60 100 3 I 17MBTC03 Molecular Biology 4 – 3 40 60 100 3 I 17MBTC04 Biophysical Techniques 4 – 3 40 60 100 3 I 17MBTC05 Practicals I – Analytical Techniques in – 6 6 40 60 100 4 Biotechnology I 17MBTC06 Practicals II – Cell Biology and Microbial – 5 6 40 60 100 3 Techniques Library 1 – – – – – – II CSS 2 – – – – – – Second Semester I 17MBTC07 Genetics 4 – 3 40 60 100 3 I 17MBTC08 Animal Biotechnology 3 – 3 40 60 100 3 I 17MBTC09 Bioinformatics 3 – 3 40 60 100 3 I 17MBTC10 Recombinant DNA technology 3 – 3 40 60 100 3 I 17MBTC11 Practicals III – Animal Biotechnology and – 4 6 40 60 100 3 Bioinformatics I 17MBTC12 Practicals IV – Recombinant DNA – 6 6 40 60 100 3 Technology I 17MBTC13 Mini Project 1 – – 100 – 100 2 I Interdisciplinary Course 4 – 3 40 60 100 4 II CSS 2 – 3 1 II Professional Certification – – – – – – 2 Internship during summer vacation for one month 1 Hrs of instruct- Scheme of Examination tions / Name of Paper / week Subject Component Part Code T P CE CIA Total Credit of Exam Duration Duration Third Semester I 17MBTC14 Immunology and Immunotechnology 4 – 3 40 60 100 3 I 17MBTC15 Plant Biotechnology 4 – 3 40 60 100 3 I 17MBTC16 Enzymes and Bioprocess Technology 4 – 3 40 60 100 3 I 17MBTC17 Pharmaceutical Biotechnology 4 – 3 40 60 100 3 I 17MBTC18 Practicals V – Immunotechnology and Plant – 5 6 40 60 100 3 Biotechnology I 17MBTC19 Practicals VI – Enzymes and Bioprocess – 6 6 40 60 100 4 Technology I 17MBTC20 Environmental Biotechnology (Self Study) 1 – 3 40 60 100 4 I Multidisciplinary Course 2 – 3 100 – 100 2 II 17MBTC21 Internship – – – 100 100 2 Fourth Semester I 17MBTC22 Food Biotechnology and 3 – 3 40 60 100 3 Nanobiotechnology I 17MBTC23 Biostatistics and Research Methodology 4 – 3 100 – 100 3 (Open Book Test) I 17MBTC24 Thesis 23 – – 100 100 200 6 Total 2700 85 Other courses to be undergone by the students: MOOC course – 2 credits Note: Minimum 85 credits to earn the degree Other courses offered by the department IDC offered by the Department of Biotechnology IDC – 17MBTI 01 Prospects of Biotechnology MDC offered by the Department of Biotechnology MDC – 17MBTM01 Scientific and Technical Writing 2 Biochemical Concepts for Biotechnology Semester I Hours of Instruction / week : 4 17MBTC01 No. of credits : 3 Objectives : 1. To understand the basic biological concepts involved in Biotechnology 2. To understand the chemical principles in the biological systems 3. To study the properties and the importance of the major small molecules and macromolecules in the biological system. Unit I – Introduction 12 hrs Properties of water – its effect on dissolved molecules – weak interactions – ionization of water – weak acids and weak bases – pH and its importance – biological buffers Concepts of bioenergetics, thermodynamics in biological system – Electron transport chain – oxidative phosphorylation Introduction to metabolism – anabolism and catabolism Self study : Chemical bonds – physical interactions – viscosity, diffusion, osmotic pressure, surface tension Unit II – Carbohydrates 12 hrs Structure and functions of biologically important mono-, di-, oligo- and polysaccharides – metabolism of carbohydrates – glycolysis, TCA cycle, glycogenesis, glycogenolysis and pentose phosphate pathway. Self study : Gluconeogenesis Unit III – Amino acids and Proteins 12 hrs Amino acids – classification and structure – peptide bond formation – biologically important peptides – insulin, oxytocin, vasopressin, glutathione Proteins – Orders of protein structure – primary, secondary, supersecondary, tertiary and quaternary – Ramachandran plot – conformation of proteins Protein catabolism – urea cycle. Metabolism of one carbon compounds, deamination, decarboxylation, transamination, transmethylation – denaturation of proteins Self study : Positive and negative nitrogen balance; Protein Energy Malnutrition (PEM) Unit IV – Lipids 12 hrs Classification and chemistry of lipids, structure and metabolism of fatty acids – fatty acid interconversion – outline of triacylglycerol and lipoprotein metabolism – cholesterol metabolism. Self study : Interrelationship between carbohydrate, protein and lipid metabolism. Unit V – Nucleic acids and Vitamins 12 hrs Structure and functions of DNA – nitrogenous bases, nucleosides, nucleotides – biologically important nucleotides – Watson and Crick model of DNA structure – A, B and Z forms of DNA – triple and quadruple structures of DNA – supercoiling of DNA – Properties of DNA – buoyant density, viscosity, denaturation, Cot curves Major and minor classes of RNA – structure and function of RNA types Chemistry of vitamins and coenzymes – NAD+, NADP+, FMN, FAD, CoASH, TPP, PLP, Biotin, Tetrahydrofolate and coenzyme B12. Self study : Disorders linked to disturbances in nucleic acid metabolism – gout Total 60 hrs 3 References: 1. Berg, J., Tymoezko, J.L. and Stryer, L. (2008) Biochemistry, 6th Edition, W.H. Freeman Publishing Company, New York. 2. Boyer, R. (2006) Concepts in Biochemistry, 3rd Edition, John Wiley and Sons Inc. 3. Devlin, T.M. (2010) Textbook of Biochemistry with clinical correlations, 7th Edition, John Wiley and Sons Inc. Publications. 4. Mathews, C.K., VanHolde, K.E. and Ahem, K.G. (2000) Biochemistry, 3rd Edition, Benjamin Cummings Publishing Co., Inc., U.K. 5. Mckee, T. and Mckee, J.M. (2003) Biochemistry – The Molecular Basis of Life, 4th Edition, Oxford University Press. 6. Murray, R.K, Bender, D.A, Botham, K.M., Kennelly, P.J., Rodwell, V.W. and Weil, P.A. (2009) Harper’s Illustrated Biochemistry, 28th Edition, The McGr.w-Hill Companies. 7. Nelson, D.L and Cox, M.M. (2008) Lehninger’s Principles of Biochemistry, 5th Edition W.H. Freeman Publishers, New York. 8. Voet, D. and Voet, J.G. (2010) Biochemistry, 4th Edition, John Wiley and Sons, New York. 4 Cell Biology and Microbiology Semester I Hours of Instruction / Week : 4 17MBTC02 No. of credits : 3 Objectives : 1. To understand the basis of cell organization and how eukaryotic cells work at the molecular level 2. To provide an overview of cell structure and function, including the flow of information from genes to proteins, and regulation of cellular processes, signalling and proliferation of cells 3. To describe how antibiotics work by interfering with cellular processes 4. To discuss the importance of microbes in ecological and industrial processes and to explain the basis of microbial pathogenesis and the host response to microbial infection Unit I –Cell Organization 12 hrs Structural differences between prokaryotic and eukaryotic cells – structure and functions of sub-cellular organelles – mitochondria, endoplasmic reticulum, Golgi complex, ribosomes, lysosomes, peroxisomes, glyoxysomes, chloroplasts, cytoskeletal elements – cytoplasm – ultrastructure of nucleus – nuclear envelope, chromatin, nucleolus Self study : From molecules to the first cell – prokaryotic and eukaryotic cell organization Unit II - Membrane structure and function 12 hrs Prokaryotic and eukaryotic cell envelopes – plasma membrane – lipid bilayer – fluid mosaic model – rafts (elementary concepts only) – types of cell junctions – CAMs, ECM – transport across membranes – pinocytosis, phagocytosis, permeability of water and salts, salt antagonism, co-transport and active transport – ATPase, ABC transporters, ion channels, ionophores, aquaporins. Self study : Characteristics of ribosomes in eukaryotic cytoplasm, mitochondria and in prokaryotic cells Unit III – Cell division, differentiation, cell cycle and cell death 12 hrs Cell division – amitosis, mitosis, meiosis – Cell differentiation – stem cells (elementary concepts only) – Cell cycle – phases, regulation, checkpoints – Cell death – necrosis, apoptosis, autophagy Self study : Types of cell death other than necrosis, apoptosis and autophagy Unit IV – Microbial taxonomy 12 hrs Classification of microorganisms – Characteristics of bacteria, fungi, algae, lichens and protozoa. Growth, culture and maintenance of microorganisms – Nutritional requirements – Uptake of nutrients – Microbial growth and measurement – Kinetics of growth Self study : Discovery and origin of microbial world Unit V – Culture media and types 12 hrs Techniques of pure culture isolation – Control of microorganisms – Sterilization and disinfection. Microbial pathology– identifying features of pathogenic bacteria, fungi and viruses,mechanism of microbial pathogenicity, etiology and pathology of common microbial diseases and currently recommended therapies for common bacterial, fungal & viral infections Self study : Types of microbial nutrition – phototrophs, chemotrophs, lithotrophs, organotrophs Total – 60hrs 5 References 1. Gupta, H.L and Jangir, H.L. (2010) Cell biology: Fundamentals and Applications, Agrobios, Jodhpur, India. 2. Plopper, G., Sharp,D. and Sikorski, E. (2015) Lewin’s cells, 3rd edition, Jones & Bartlett Publishers, Massachusetts 3. Cooper, G.M and Robert (2007) The Cell: a Molecular Approach, 4th Edition, ASM Press. 4. Gupta, P.K. (2005) Cell and Molecular Biology, 1st Edition, Rastogi Publications, Meerut. 5. Karp, G. (2010) Cell Biology, 3rd Edition, John Wiley&Sons, New Jersey, United States 6. Tortora, G.J.,