Wound Repair and Regeneration Wound Healing Society Tentative Schedule of Abstract Presentations April 28 to May 1, 2007 Tampa Convention Center SUNDAY, APRIL 29, 2007 Oxygen/Oxidative Stress Session Number: 46 Burn/Fetal Wounds/Acute Wounds Monday, April 30, 2007, 4:00 to 6:00 pm Session Number: 28 Abstracts 57–65 Sunday, April 29, 2007, 5:00 to 7:00 pm Abstracts 1–10 TUESDAY, MAY 1, 2007 Bioengineering/Extracellular Matrix Angiogenesis Session Number: 29 Session Number: 51 Sunday, April 29, 2007, 5:00 to 7:00 pm Tuesday, May 1, 2007, 7:30 to 9:30 am Abstracts 11–19 Abstracts 66–74 Fibrosis/Scar Inflammation Session Number: 30 Session Number: 52 Sunday, April 29, 2007, 5:00 to 7:00 pm Tuesday, May 1, 2007, 7:30 to 9:30 am Abstracts 20–29 Abstracts 75–84 Clinical Treatments Session Number: 53 MONDAY, APRIL 30, 2007 Tuesday, May 1, 2007, 7:30 to 9:30 am Abstracts 85–93 Young Investigator Awards Session Number: 41 ORAL-POSTER PRESENTATIONS: Monday, April 30, 2007, 2:30 to 3:45 pm Abstracts 30–37 Blue Ribbon Posters Sunday, April 29, 2007, 7:00 to 8:30 am Gene Therapy/Growth Factors Abstracts 94–102 Session Number: 44 Monday, April 30, 2007, 4:00 to 6:00 pm Abstracts 103–149 (Posters will be on display at all Abstracts 38–47 times) Even Numbered Poster Oral Presentations Chronic Wounds Sunday, April 29, 2007, 7:00 to 8:30 am Session Number: 45 Monday, April 30, 2007, 4:00 to 6:00 pm Odd Numbered Poster Oral Presentations Abstracts 48–56 Monday, April 30, 7:00 to 8:30 am Wound Rep Reg (2007) 15 A13 c 2007 by the Wound Healing Society A13 Wound Repair and Regeneration Abstracts Wound Healing Society Tentative Schedule of Abstract Presentations April 28 to May 1, 2007 Tampa Convention Center SUNDAY, APRIL 29, 2007 2 SPECTRAL RESOLUTION OF WATER CONCENTRATION Burn/Fetal Wounds/Acute Wounds (EDEMA) IN BURNED TISSUE Session Number: 28 K.M. Cross1, MD, M.A. Levasseur2, EET M.G. Sowa2, PhD, L. Leonardi2, Sunday, April 29, 2007, 5:00 to 7:00 pm 1 J.S. Fish , MD, MSC Abstracts 1–10 1University of Toronto 2 1 National Research Council-Institute of Biodiagnostics DIFFERENTIAL SPHINGOSINE KINASE EXPRESSION IN Introduction: An increase in vascular permeability causes edema formation MID- VERSUS LATE-GESTATIONAL FETAL MOUSE post thermal injury. Near Infrared Spectroscopy (NIRS) measures water EXCISIONAL WOUNDS concentration in tissue and was utilized in this study to assess edema formation 1 in acute partial thickness burn wounds. Stephanie R. Goldberg, Gerald L. Quirk, Virginia W. Sykes, Tomasz Kordula, Methods: Adult burn patients o 72 hours post injury, thermal and o 20% and David A. Lanning 1 BSA were eligible. Burns were stratified based on histology/clinical outcomes Division of Pediatric Surgery/Department of Surgery and, Department of into partial thickness superficial (PTS) and partial thickness deep (PTD) Biochemistry, Virginia Commonwealth University Health System, wounds. Burn and control site measures were obtained using a handheld NIRS Richmond, VA probe that can measure water concentration. Paired t-tests compared burn and Introduction: Sphinogosine-1-Phosphate (S1P) is a sphingolipid that modulates control sites. Independent t-tests compared the water concentration in PTS versus PTD for burn and control sites. Results are mean SD with statistical angiogenesis, regulates apoptosis, and is a mitogen for fibroblasts; however, the Æ vulnerary role of S1P has not been elucidated. Previously, we developed a fetal significance achieved at p o 0.05. model of wound healing in which mid-gestational excisional wounds closed Results: The sample population, SPT (n = 15) and DPT (n = 8) burns, did not differ with respect to age (40 15 yr), BSA (5 4%), APACHE II (3 5), and rapidly compared to late-gestational wounds and without scar formation. We Æ Æ Æ hypothesized that mid-gestational wound closure would be associated with mean time for NIRS assessment (2 days). Water concentration was increased in burned tissue compared to control regions (7.9E À 001 1.3E À 001 vs. altered expression of sphingosine kinase (SphK) 1 and/or 2, the enzymes that Æ 5.5E À 001 Æ 1.8E À 001 mM, p = 0.0001). PTD burns showed an increase in water catalyze the phosphorylation of sphingosine to S1P. À 001 À 001 Methods: Time-dated, pregnant FVB mice underwent laparotomy and hyster- concentration compared to PTS burns (8.7E Æ 1.3E vs. 7.5E À 001 Æ 1.1E À 001 mM, p = 0.005). The water concentration for the control otomy at mid-(E15) and late-gestational (E18) ages (term = 20–21 days). À 001 À 001 Full-thickness, excisional wounds (2.5 mm) were made on up to 4 fetuses/doe. sites was similar for PTS and PTD (5.3E Æ 1.8E vs. 5.9E À 001 1.8E À 001 mM, p = 0.417). Wounds were harvested with 1 mm of surrounding skin at 32 and 48 hours. Æ SphK1 and SphK2 gene expression were measured by real-time PCR and Conclusion: NIRS detected local differences in edema formation with DPT burns showing an increase in edema formation compared to viable partial normalized to GAPDH. Control tissue consisted of normal fetal skin. Student’s thickness burns. The capacity to objectively measure edema formation in tissue paired t-test was performed for statistical analysis. Results: SphK1 expression was increased in E18 wounds compared to E18 could serve as a guide to fluid resuscitation and provides insight into the fluid shift that occurs in the acute burn period. normal skin and E15 wounds at 32 hours (table). However, at 48 hours SphK1 expression was increased in E15 wounds compared with E18 wounds. Tran- Grant Info: The Physicians’ Services Incorporated Foundation. script levels for SphK2 were increased in the wounds compared with the normal skin in the E15 and E18 groups at 32 hours, yet no differences were seen at 48 hours. 32 hours 48 hours n SphK1 SphK2 n SphK1 SphK2 m E15 normal skin 8 1.8 Æ 0.2 3.8 Æ 0.4 11 2.3 Æ 0.4 1.0 Æ 0.1 m E15 wound 6 2.9 Æ 0.7Ã 7.3 Æ 1.7 10 1.8 Æ 0.2 1.1 Æ 0.1 w E18 normal skin 7 2.2 Æ 0.3Ã,# 3.3 Æ 0.5 15 1.3 Æ 0.4 1.1 Æ 0.4 w E18 wound 4 5.6 Æ 0.9Ã,# 5.6 Æ 0.9 8 0.8 Æ 0.2ÃÃ 0.8 Æ 0.1 Ãp = 0.023 #pwp = 0.007 mp = 0.05 ÃÃp = 0.01 Conclusions: SphK1 and SphK2 are differentially expressed in mid- and late- gestational mouse wounds. SphK1 expression is increased in late-gestational wounds at 32 hours but mid-gestational wounds at 48 hours, whereas SphK2 expression is increased in mid- and late-gestational wounds at 32 hours only. Whether increased SphK1 and/or SphK2 transcript levels results in sustained S1P at the wound site has yet to be determined. However, since SphK1 can directly prevent apoptosis through the activation of nuclear factor kB, it may also contribute directly to the rapid mid-gestational wound closure. This work was supported by a grant from the Jeffress Memorial Trust J751 (DAL) and the NIH F32 GM078977-01 (SRG). A14 Wound Rep Reg (2007) 15 A14–A54 c 2007 by the Wound Healing Society Abstracts 3 5 FREEZING ALTERS THE VISCOELASTIC RESPONSE OF WOUND COVERAGE WITH MICROBIAL NANOCELLULOSE UNINJURED SKIN AND HEALING WOUNDS IN TREATMENT OF DONOR SITE WOUNDS AND SKIN D.T. Corr1,2, C.L. Gallant-Behm2, N.G. Shrive2, D.A. Hart2 AUTOGRAFTS AFTER SEVERE BURNS 1Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, Ludwik K. Branski1, Wojciech Czaja2, Marc G. Jeschke1, 2The McCaig Centre, University of Calgary, Calgary, AB, Canada William B. Norbury1, Oscar E. Masters1, Yoshimitsu Nakano1, Daniel L. Taber1, R. Malcolm Brown2 and David N. Herndon1 Aim: To determine if the practice of freezing skin, for storage and later testing, 1Shriners Hospital for Children and University of Texas Medical Branch, alters its viscoelastic behavior, and the influences of wounding and direction- Galveston, Texas, ality on that behavior. We hypothesized that freezing would change the 2University of Texas at Austin absolute material property values significantly, but would not change the relative effect of specimen orientation or scarring. Introduction: In severely burned patients, early wound closure is essential and Methods: Tissue was harvested 70 days following full thickness wounding in the choice of the correct dressing paramount. Microbial Nanocellulose (NC), juvenile female domestic pigs (N = 12). Skin sections were halved, one half an inert material produced by Acetobacter xylinum, has emerged as a new tested fresh, and the others frozen and stored at À 80 1C for subsequent testing. coverage material that is very conformable, can hold a large amount of liquid, Samples were obtained in the cranial-caudal and dorsal-ventral directions, for and retains a high mechanical strength, therefore making it an ideal wound both scar tissue and uninjured skin, and were evaluated using in vitro stress dressing. Our aim was to compare NC and standard dressing material in a relaxation in an INSTRON universal test machine. Frozen tissue was thawed porcine burn wound model. slowly, and equilibrated to room temperature prior to testing. Specimens were Materials and Methods: Third degree burn wounds (Treatment: n = 10, con- kept moist throughout testing. trol: n = 8) were created using hot aluminum, excised 24 h post injury and Results: Frozen skin produced similar values to those of fresh skin in many grafted with autologous skin transplant (4:1 meshgraft, wound size: 50 cm2).