Product Information Sheet for HM-158 Ralstonia sp., Strain 5_2_56FAA Incubation: Temperature: 30°C Atmosphere: Aerobic Catalog No. HM-158 Propagation: 1. Keep vial frozen until ready for use, then thaw. For research use only. Not for human use. 2. Transfer the entire thawed aliquot into a single tube of broth. Contributor: 3. Use several drops of the suspension to inoculate an Emma Allen-Vercoe, Assistant Professor, Department of agar slant and/or plate. Molecular and Cellular Biology, University of Guelph, Guelph, 4. Incubate the tube, slant and/or plate at 30°C for 48 Ontario, Canada hours. Manufacturer: Citation: BEI Resources Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH as Product Description: part of the Human Microbiome Project: Ralstonia sp., Strain Bacteria Classification: Burkholderiaceae, Ralstonia 5_2_56FAA, HM-158.” Species: Ralstonia sp. Strain: 5_2_56FAA Biosafety Level: 2 Original Source: Ralstonia sp., strain 5_2_56FAA was Appropriate safety procedures should always be used with isolated from inflamed tissue taken from the terminal ileum this material. Laboratory safety is discussed in the following 1 of a 29-year-old female patient with Crohn's disease. publication: U.S. Department of Health and Human Services, Comments: Ralstonia sp., strain 5_2_56FAA (HMP ID 0989) Public Health Service, Centers for Disease Control and is a reference genome for The Human Microbiome Project Prevention, and National Institutes of Health. Biosafety in (HMP). HMP is an initiative to identify and characterize Microbiological and Biomedical Laboratories. 5th ed. human microbial flora. The complete genome of Ralstonia Washington, DC: U.S. Government Printing Office, 2009; see sp. 5_2_56FAA is currently being sequenced at the Broad http://www.cdc.gov/biosafety/publications/bmbl5/index.htm. Institute (GenBank: ACTT00000000). Note: HMP material is taxonomically classified by the Disclaimers: depositor. Quality control of these materials is only You are authorized to use this product for research use only. performed to demonstrate that the material distributed by It is not intended for human use. BEI Resources is identical to the deposited material. Use of this product is subject to the terms and conditions of Ralstonia species, previously included in the genus the BEI Resources Material Transfer Agreement (MTA). The Pseudomonas, are Gram-negative, aerobic, motile, rod- MTA is available on our Web site at www.beiresources.org. shaped, free-living bacteria that are common inhabitants of soil, water, and vegetation.2,3 Many Ralstonia species While BEI Resources uses reasonable efforts to include represent opportunistic pathogens to humans, animals, or accurate and up-to-date information on this product sheet, plants. neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations Material Provided: from scientific literature and patents are provided for Each vial contains approximately 0.5 mL of bacterial culture informational purposes only. Neither ATCC® nor the U.S. in 0.5X Tryptic Soy Broth supplemented with 10% glycerol. Government warrants that such information has been confirmed to be accurate. Note: If homogeneity is required for your intended use, please purify prior to initiating work. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. Packaging/Storage: ATCC® and the U.S. Government are not liable for any HM-158 was packaged aseptically, in screw-capped plastic damages or injuries arising from receipt and/or use of this cryovials. The product is provided frozen and should be product. While reasonable effort is made to ensure stored at -60°C or colder immediately upon arrival. For long- authenticity and reliability of materials on deposit, the U.S. term storage, the vapor phase of a liquid nitrogen freezer is Government, ATCC®, their suppliers and contributors to BEI recommended. Freeze-thaw cycles should be avoided. Resources are not liable for damages arising from the misidentification or misrepresentation of products. Growth Conditions: Media: Use Restrictions: Tryptic Soy Broth or equivalent This material is distributed for internal research, non- Tryptic Soy Agar or equivalent commercial purposes only. This material, its product or its BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898 © 2012 American Type Culture Collection (ATCC). All rights reserved. HM-158_26JAN2012 Page 1 of 2 Product Information Sheet for HM-158 derivatives may not be distributed to third parties. Except as performed under a U.S. Government contract, individuals contemplating commercial use of the material, its products or its derivatives must contact the contributor to determine if a license is required. U.S. Government contractors may need a license before first commercial sale. References: 1. HMP 0989 (Ralstonia sp., strain 5_2_56FAA) 2. Yabuuchi, E., et al. “Transfer of Two Burkholderia and an Alcaligenes Species to Ralstonia gen. nov.: Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov.” Microbiol. Immunol. 39 (1995): 897-904. PubMed: 8657018. 3. Chen, W.-M., et al. “Ralstonia taiwanensis sp. nov., Isolation from Root Nodules of Mimosa species and Sputum of a Cystic Fibrosis Patient.” Int. J. Syst. Evol. Microbiol. 51 (2001): 1729-1735. PubMed: 11594603. ATCC® is a trademark of the American Type Culture Collection. BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898 © 2012 American Type Culture Collection (ATCC). All rights reserved. HM-158_26JAN2012 Page 2 of 2 .