Efficient Professional Protein and Antibody Platforms PDE1B Antibody Basic information: Catalog No.: UMA60393 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: Protein A affinity purified Useful Information: WB:1:500-1:1000 IHC:1:50-1:200 Applications: FC:1:100-1:200 Reactivity: Human, Rat Specificity: This antibody recognizes PDE1B protein. Immunogen: Recombinant protein PDEs are key enzymes in signaling pathways that influence smooth muscle tone regulation. The PDE1 family are calmodulin-dependent (CaM-PDEs) that undergo stimulation through a calcium-calmodulin complex. Human PDE1B (PDE1B1) protein is present in neuronal cells of the cerebellum, hip- pocampus, and caudate, and lymphoblastoid lines, such as RPMI-8392 cells. Description: PDE1B may participate in learning, memory, and regulation of phosphoryla- tion of DARPP-32 in dopaminergic neurons. A splice variant known as PDE1B2 encodes a 516-amino acid protein and diverges from PDE1B1 by the replacement of the first 38 residues with an alternative 18 residues. The human PDE1B gene maps to chromosome 12q13, contains 13 exons, and encodes a 536 amino acid protein. Uniprot: Q01064(Human) Q01066(Rat) BiowMW: 61 kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of PDE1B on human PDE1B recombinant protein using anti-PDE1B antibody at 1/1,000 dilution. Western blot analysis of PDE1B on HEK293 (1) and PDE1B-hIgGFc transfected HEK293 (2) cell lysate using anti-PDE1B antibody at 1/1,000 dilu- tion. Western blot analysis of PDE1B on PC-12 cell ly- sate using anti-PDE1B antibody at 1/1,000 dilu- tion. Immunohistochemical analysis of paraf- fin-embedded human ovarian cancer tissue using anti-PDE1B antibody. Counter stained with he- matoxylin. Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] .