Cryo-electron tomography of mouse hepatitis virus: Insights into the structure of the coronavirion Montserrat Ba´ rcenaa, Gert T. Oostergetelb, Willem Bartelinkc, Frank G. A. Faasa, Arie Verkleijd, Peter J. M. Rottierc, Abraham J. Kostera,1, and Berend Jan Boschc aDepartment of Molecular Cell Biology, Electron Microscopy Section, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; bBiophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9497 AG, Groningen, The Netherlands; cDepartment of Infectious Diseases and Immunology, Virology Division, Faculty of Veterinary Medicine, Utrecht University, 3584 CL, Utrecht, The Netherlands; and dDepartment of Molecular Cell Biology, Utrecht University, 3584 CH, Utrecht, The Netherlands Edited by Stanley Perlman, University of Iowa, Iowa City, IA, and accepted by the Editorial Board November 20, 2008 (received for review June 3, 2008) Coronaviruses are enveloped viruses containing the largest re- orchestrate virion assembly via interactions with all viral com- ported RNA genomes. As a result of their pleomorphic nature, our ponents, including itself. Virus-like particles devoid of RNP and structural insight into the coronavirion is still rudimentary, and it S can be formed upon co-expression of M and E (7). is based mainly on 2D electron microscopy. Here we report the Despite multiple biochemical and morphological studies, 3D virion structure of coronaviruses obtained by cryo-electron many fundamental aspects of the virion architecture remain to tomography. Our study focused primarily on the coronavirus be elucidated. The structure of the coronavirus RNP core has prototype murine hepatitis virus (MHV). MHV particles have a been particularly elusive. In negatively stained intact virions, the distinctly spherical shape and a relatively homogenous size (Ϸ85 interior is not visible, and thus early studies focused on samples nm envelope diameter). The viral envelope exhibits an unusual in which the viral envelope was disrupted and the inner content thickness (7.8 ؎ 0.7 nm), almost twice that of a typical biological released. RNPs extracted in this way from several CoVs appear membrane. Focal pairs revealed the existence of an extra internal to have helical symmetry (8–10). However, under certain con- layer, most likely formed by the C-terminal domains of the major ditions, intermediate spherical nucleocapsids have been re- envelope protein M. In the interior of the particles, coiled struc- ported (11, 12). These structures, which showed polygonal tures and tubular shapes are observed, consistent with a helical profiles, were devoid of S protein and lipids but, in addition to nucleocapsid model. Our reconstructions provide no evidence of a RNA and the N protein, contained the M protein (12). These shelled core. Instead, the ribonucleoprotein seems to be exten- assemblies were suggested to represent an additional viral sively folded onto itself, assuming a compact structure that tends structure: a shelled core, possibly even icosahedral, that would to closely follow the envelope at a distance of Ϸ4 nm. Focal contact enclose the helical RNP (12). In cryo-electron microscopy, in points and thread-like densities connecting the envelope and the which the contrast arises from the specimen itself, the internal ribonucleoprotein are revealed in the tomograms. Transmissible content becomes apparent in undisrupted viruses (12–14). How- gastroenteritis coronavirion tomograms confirm all the general ever, the superposition of viral features in projection images features and global architecture observed for MHV. We propose a hampers their structural interpretation. general model for the structure of the coronavirion in which our To a large extent, the pleomorphic nature of CoVs has been own and published observations are combined. the main obstacle to clarifying details of their structure, as the particles are not identical and therefore cannot be analyzed by coronaviruses ͉ enveloped viruses ͉ plus-stranded RNA viruses ͉ single particle averaging methods (15). To overcome this limi- transmissible gastroenteritis coronavirus tation, we have studied murine hepatitis virus (MHV; CoV group 2), the prototypic CoV, by cryo-electron tomography and have obtained 3D reconstructions of CoVs. The 3D nature of the oronaviruses (CoVs) are large, enveloped, plus-stranded data was particularly useful to gain insight into the inner RNA viruses that infect a wide variety of avians and C structure of the intact virus. As cyro-electron tomography mammals. Several CoVs are human pathogens, including the provides a 3D structure for each individual virus particle in the causative agent of severe acute respiratory syndrome (SARS) (1, sample, our results show the morphological variations within the 2). The SARS outbreak in 2002–2003 served as a grim reminder viral population together with common structural motifs. Cryo- of these viruses’ potential to cross the species barrier and tomography of transmissible gastroenteritis coronavirus triggered a renewed interest in CoVs. Since then, new CoVs have (TGEV; CoV group 1) reveals a similar virion organization, come to expand the list of discovered members, with two more suggesting a general model for the architecture of CoVs. respiratory human CoVs among them (3, 4). The current virion model of CoVs, based on morphological Results and biochemical data, depicts a pleomorphic particle that is Cryo-Electron Tomography of MHV. Purified MHV samples were roughly spherical but shows variations in size (80–120 nm) and rapidly cryo-fixed by plunge freezing. A total of 7 tilt series were shape (reviewed in refs. 5, 6). The virion contains the ribonu- collected at a relatively low defocus (4 ␮m) to preserve fine cleoprotein (RNP) core, consisting of a genomic RNA that is the largest among all RNA viruses (Ϸ30 kb), in complex with the nucleocapsid (N) protein (Ϸ380–450 aa). The RNP is sur- Author contributions: M.B., P.J.M.R., A.J.K., and B.J.B. designed research; M.B., G.T.O., W.B., rounded by a lipidic envelope. Three viral proteins are anchored and B.J.B. performed research; G.T.O., F.G.A.F., and A.V. contributed new reagents/analytic in the envelope of all CoVs: the abundant triple-spanning tools; M.B., F.G.A.F., A.J.K., and B.J.B. analyzed data; and M.B. wrote the paper. membrane (M) protein (Ϸ230 aa), the envelope (E) protein The authors declare no conflict of interest. (Ϸ100 aa; present in only small amounts), and the spike (S) This article is a PNAS Direct Submission. S.P. is a guest editor invited by the Editorial Board. protein (Ϸ1,300 aa), forming trimeric spikes. The spikes are Freely available online through the PNAS open access option. responsible for receptor binding and membrane fusion and 1To whom correspondence should be addressed. E-mail: [email protected]. extend radially from the envelope, giving the virions a solar This article contains supporting information online at www.pnas.org/cgi/content/full/ image to which coronaviruses owe their name. The M protein 0805270106/DCSupplemental. appears to be the building brick of the virion and is thought to © 2009 by The National Academy of Sciences of the USA 582–587 ͉ PNAS ͉ January 13, 2009 ͉ vol. 106 ͉ no. 2 www.pnas.org͞cgi͞doi͞10.1073͞pnas.0805270106 Downloaded by guest on September 28, 2021 A B C Fig. 3. (A) Slice through one of the reconstructed MHV particles (5.8 nm Fig. 1. Virtual slice (8.7 nm thick) in one of the reconstructed fields of thick). The arrows point to some of the granular densities visible in the virion purified MHV particles. Inset, Z-slices (5.8 nm thick) through one of the MHV interior. These are likely to correspond to the N protein in complex with RNA. cryo-tomograms. (Scale bars, 100 nm.) The dashed circle encloses a quasi-circular profile formed by these densities. (A). Serial sections (4 nm thick) of this region encompassing a total height of 11 nm. (B and C) Thick virtual slices (11 nm thick) of four different MHV detail contrast transfer. Fig. 1 shows one of the reconstructed particles displaying (B) circular profiles and (C) tubular fragments. These patterns are colored in blue. Assuming that they are favorable orientations of areas, illustrating the typical distribution and general preserva- Ϸ tion of the particles in the preparations (see also Movie S1 and the RNP coil, a number of 4 to 5 granular densities per turn and a pitch of 10 nm were estimated. (Scale bars, 50 nm.) Movie S2). Three-dimensional density maps of 144 virions were computed from these data sets. differently sized MHV particles found in our preparations are General Features of MHV. The shape of the reconstructed MHVs shown in the gallery of Fig. 2. The viruses share a similar appeared to be remarkably homogeneous. The large majority of appearance and a detailed analysis did not reveal size-dependent the virions exhibit a distinct spherical form (Fig. 1). This is in differences in any of the viral features discussed in the next MICROBIOLOGY contrast not only with early studies of negatively stained CoVs, sections. in which distortions were known to account for part of the heterogeneity observed, but also with previous cryo-electron Ribonucleoprotein Complex. Our reconstructions show the interior microscopy images in which CoVs appear elliptical (14). Only of MHV particles to be filled with material. In some instances, Ϸ 7% of the MHVs in our samples showed ellipticity; however, however, a central region of lower density was observed (see Fig. our data strongly suggest this to be a result of distortions induced 2). Although these areas are more common in larger particles, in the proximity of the water-air interface (Fig. S1). Therefore, their presence and extent are not systematically correlated with the subsequent analysis focused on the remaining 135 spherical the virion size. Except for these lower-density regions, the RNP virions. seems to be relatively densely packed and disorganized under- The size range of MHV particles is shown in Fig.