Proceedings of the American Association of Veterinary Laboratory Diagnosticians 50th Annual Conference John Ascuaga’s Nugget Reno, Nevada October 18-23, 2007 American Association of Veterinary Laboratory Diagnosticians The American Association of Veterinary Laboratory Diagnosticians (AAVLD) is a not-for-profit professional organization which seeks to: • Disseminate information relating to the diagnosis of animal diseases • Coordinate diagnostic activities of regulatory, research and service laboratories • Establish uniform diagnostic techniques • Improve existing diagnostic techniques • Develop new diagnostic techniques • Establish accepted guidelines for the improvement of diagnostic laboratory organizations relative to personnel qualifications and facilities • Act as a consultant to the United States Animal Health Association on uniform diagnostic criteria involved in regulatory animal disease programs *********************************** AAVLD Officers, 2007 President.................................................................................................... Barbara Powers, Fort Collins, CO President-elect..........................................................................................Grant Maxie, Guelph, ON, Canada Vice-president.....................................................................................................David Steffen, Lincoln, NB Secretary-Treasurer.................................................................................................. Alex Ardans, Davis, CA Immediate Past-President................................................................................Donal O’Toole, Laramie, WY AAVLD Executive Board, 2007 President.................................................................................................... Barbara Powers, Fort Collins, CO President-elect....................................................................................................... Grant Maxie, Guelph, ON Vice-president.....................................................................................................David Steffen, Lincoln, NB Secretary-Treasurer.................................................................................................. Alex Ardans, Davis, CA Northeast...................................................................................................................Bruce Akey, Ithaca, NY Southeast............................................................................................................. Ron Wilson, Nashville, TN North-central................................................................................................................ Neil Dyer, Fargo, ND South-central.....................................................................................................Richard Mock, Amarillo, TX Northwest......................................................................................................Timothy Baszler, Pullman, WA Southwest..........................................................................................................Robert Poppenga, Davis, CA Canada Provincial ......................................................................................... Maria Spinato, Abbotsford, BC Canada Federal..................................................................................................Shane Renwick, Ottawa, ON NVSL ........................................................................................................................Beth Lautner, Ames, IA For membership/subscription information, please contact AAVLD Secretary-Treasurer Dr. Alex Ardans Fax 530-752-5680 P.O. Box 1770 Email [email protected] Davis, CA 95617 Phone - 530-754-9719 *********************** _____________________________________________________________________________________ AAVLD Annual Conference Proceedings - 2 - Reno, NV, October, 2007 Our thanks to all of our AAVLD 2007 exhibitors and sponsors!! AAVLD 2007 sponsors (please see ads, pp 205-209) Platinum ($5,000) Bio-Rad Laboratories Gold ($2,500) Applied Biosystems Silver ($1,000) Novartis Hydrol-Pro Technologies, Inc. Trek Diagnostic Systems VMRD Bronze ($500) Midland BioProducts Corporation Newport Labs AAVLD 2007 exhibitors (directory and floor plan, pp 210-217) AbD Serotec Laboratory Automation Solutions, Inc. Advanced Technology Corp. (ATC) (LAS) Aperio Technologies Midland BioProducts Corporation BD Diagnostics National Institute for Animal Agriculture Bio-Rad Laboratories (NIAA) Bio SAFE Engineering, Inc. Prionics USA, Inc. BioTrove Qiagen, Inc. Calbiotech, Inc. Quality Systems Integrators Centaur, Inc. Roche Applied Science Cepheid Saunders/Mosby/Churchill Corbett Robotics, Inc. Synbiotics Corporation Crawford Industrial Group Tetracore, Inc. Fair Isaac Corp Trek Diagnostic Systems Global Vet Link USDA National Animal Health Emergency Harley Ellis Devereaux Response Corps (NAHERC) Hydrol-Pro Technologies, Inc. Ventana Medical Systems, Inc. IDEXX Laboratories, Inc. Veterinary Laboratories Agency Immuno-Mycologics, Inc. VMRD, Inc. Inverness Medical Professional Diagnostics Whatman, Inc. _____________________________________________________________________________________ AAVLD Annual Conference Proceedings - 3 - Reno, NV, October, 2007 Acknowledgments The success of a meeting is a function of both presenters and attendees. A special thank you to all who presented their data and findings, all exhibitors and sponsors, and everyone who attended the meeting. A special thank you also to all of our invited speakers for the AAVLD Plenary Session and the USAHA- AAVLD Scientific Session. And as a special feature of the 50th Annual Conference, thank you to all of the Pope Award winners and Life Members who were able to attend and make this meeting unique. The Program Committee, listed below, deserves a special acknowledgement for all of their hard work, organization, review and editing of the abstracts, and moderation of sessions. Jay Kammerzell was again instrumental in computerizing and organizing the review process. Pat Blanchard, Allison Reitz, Vanessa Garrison, Jackie Cassarly, and Linda Ragland (USAHA) coordinated the meeting room arrangements, exhibitor booth arrangements, sponsor agreements, breaks, and all of the many other details that go into making a meeting a success. ****************************************** AAVLD Program Committee, 2007 Grant Maxie, Chair John Adaska Catherine Barr Timothy V. Baszler Steven R. Bolin Letitia Curley Francois C. Elvinger Scott D. Fitzgerald Sharon K. Hietala Lorraine J. Hoffman Hong Li J. Lindsay Oaks, Jr. Kristy Lynn Pabilonia Carlos Reggiardo J. Glenn Songer Dave Steffen Patricia A. Talcott Kyoung-Jin Yoon ************************************* Please note: Abstracts published in these proceedings were peer reviewed by the Program Committee to determine that data supporting conclusions is likely to be presented, and were edited into a consistent format for publication. Full manuscripts were not evaluated and readers should contact the author for referral to a full presentation of data or for permission to use, copy, or distribute data contained in an abstract. _____________________________________________________________________________________ AAVLD Annual Conference Proceedings - 4 - Reno, NV, October, 2007 AAVLD Plenary Session Saturday, Oct 20, 2007 Ponderosa B “Past, present and future of veterinary laboratory medicine” Moderator: Grant Maxie 07:30 AM Welcome - Grant Maxie, President-Elect, AAVLD Page David Steffen, Vice-President, AAVLD 07:35 AM The evolution of the AAVLD - Robert Crandell, Larry Morehouse, Vaughn Seaton ........ 25 08:15 AM Veterinary diagnostic toxicology: from spots to peaks to fragments and beyond (or why does diagnostic toxicology cause economic heartburn for laboratory directors?) - Robert Poppenga, Mike Filigenzi, Elizabeth Tor, Linda Aston, Larry Melton, Birgit Puschner................................................................................... 26 08:45 AM Microspheres and the evolution of testing platforms - Susan Wong ............................... 27 09:15 AM BREAK 09:45 AM A production management (client’s) perspective on diagnostics - Dale Grotelueschen........................................................................................................................ 28 10:15 AM AAVLD survey of pet food-induced nephrotoxicity in North America, April to June, 2007 - Wilson Rumbeiha, Dalen Agnew, Grant Maxie, Michael Scott, Brent Hoff, Barbara Powers............................................................................................................ 29 10:45 AM Ecosystem health, agriculture, and diagnostic laboratories: challenges and opportunities - Thomas Besser ............................................................................................ 30 11:15 AM House of Delegates _____________________________________________________________________________________ AAVLD Annual Conference Proceedings - 5 - Reno, NV, October, 2007 Virology Scientific Session Saturday, October 20, 2007 Bonanza A Moderators: Kyoung-Jin Yoon, Kristy Lynn Pabilonia 1:00 PM Further improvement and validation of MagMAX-96 AI/ND viral RNA isolation kit for efficient removal of RT-PCR inhibitors from cloacal swabs and tissues for rapid diagnosis of avian influenza virus by real-time reverse transcription PCR - Amaresh Das, Erica Spackman, Mary J. Pantin-Jackwood, David E. Swayne, David Suarez .........................................................................................................................