TOC From Bench to Bountiful Harvests Annual Report Multinational Arabidopsis Steering Committee (MASC) 2014/2015 TOC The Multinational Arabidopsis Steering Committee Annual Report 2014/2015 Nicholas Provart [email protected] MASC Chair Loïc Lepiniec [email protected] MASC Co-chair Luise H. Brand [email protected] MASC Coordinator, Executive Secretary Ruth Bastow [email protected] Minami Matsui [email protected] Malcolm Bennett [email protected] Sean May [email protected] Dario Bonetta [email protected] Blake Meyers [email protected] Siobhan Brady [email protected] José Luis Micol [email protected] Jelena Brkljacic [email protected] Moritz K. Nowack [email protected] Lynette Brownfield [email protected] Michael Palmgren [email protected] Ana I. Caño-Delgado [email protected] Catherine Perrot- Jorge J. Casal [email protected] Rechenmann [email protected] Marcos Castellanos-Uribe [email protected] Leonore Reiser [email protected] Sacco De Vries [email protected] Roland Pieruschka [email protected] Brian Dilkes [email protected] J. Chris Pires [email protected] Joe Ecker [email protected] Kazuki Saito [email protected] Maria E. Eriksson [email protected] Sigal Savaldi-Goldstein [email protected] Fabio Fiorani [email protected] Ben Scheres [email protected] Joanna D. Friesner [email protected] Ulrich Schurr [email protected] Robert Furbank [email protected] Motoaki Seki [email protected] Erich Grotewold [email protected] Giovanna Serino [email protected] Klaus Harter [email protected] Kentaro K. Shimizu [email protected] Polydefkia Hatzopoulos [email protected] Ramamurthy Srinivasan [email protected] Marie-Theres Hauser [email protected] Keiko Sugimoto [email protected] Joshua Heazlewood [email protected] Chris Town [email protected] Eva Huala [email protected] Doris Wagner [email protected] Inhwan Hwang [email protected] Wolfram Weckwerth [email protected] Alexandra Jones [email protected] Michael Wrzaczek [email protected] Jitendra P. Khurana [email protected] Marcelo J. Yanovsky [email protected] Masatomo Kobayashi [email protected] Viktor Žárský [email protected] Marcus A. Koch [email protected] The Multinational Arabidopsis Steering Committee July 2015 1 | MASC Report 2014/15 TOC Imprint Cover design and editing Dr. Luise H. Brand (MASC coordinator), University of Tübingen, Center for Molecular Biology (ZMBP), Auf der Morgenstelle 32, 72076 Tübingen, Germany, [email protected] Cover images Modeling vascular development (top) Outcomes of simulation of auxin accumulation (green) and cytokinin (red) in a mathematical model of growth and patterning in the Arabidopsis embryonic vascular tissue. The image shows 8 indepen- dent simulations, each giving a realistic pattern of hormone response. Courtesy of Milad Adibi and Christian Fleck (page 40). Microtubules in plant pavement cells (bottom left) Microtubule orientations (green) and mechanical tensions (white) in pavement cells. Courtesy of Arun Sampathkumar and Pawel Krupinski (page 42). Root branch induced by water (bottom left center). Radial cross-section of a maize root showing the development of an early-stage root branch that was induced by local moisture. Courtesy of Neil E. Robbins II and José R. Dinneny (page 41). Arabidopsis leaf axils, where branches form (bottom right center). Confocal picture of transverse section through the meristem and leaf primordia region of Arabidopsis. LAS-GFP (green) is expressed in leaf axils, where axillary buds initiate. Courtesy of Yuling Jiao (page 40). Complex leaf shape (bottom right) From left to right: Leaf silhouette of a simple Arabidopsis thaliana leaf, Leaf silhouette of a complex leaf of Cardamine hirsuta, developing complex leaf of Cardamine hirsuta, where expression of the RCO gene is visualized in blue. Courtesy of Miltos Tsiantis (page 42). Further information www.arabidopsisresearch.org The MASC report 2014/15 and previous reports are available online at: MASC, The Multinational Arabidopsis Steering Committee: http://arabidopsisresearch.org/index.php/publications/masc-reports uNASC, The Nottingham Arabidopsis Stock Centre: http://arabidopsis.info/progreports.html TAIR, The Arabidopsis Information Resource: http://www.arabidopsis.org/portals/masc/masc_docs/masc_reports.jsp Dr. Luise Brand and Prof. Dr. Nicholas Provart wrote articles with input from Dr. Loïc Lepiniec, if no author is stated. Published by the Multinational Arabidopsis Steering Committee (MASC) July, 2015. Acknowledgements MASC is grateful to all authors for their contribution to the MASC annual report 2014/2015. This report has been written by MASC chair, co-chair, coordinator, subcommittee chairs and co-chairs with support of subcommittee members, country representatives, project or resource directors and the wider Arabidopsis community. The MASC community has been coordinated by Luise Brand. MASC thanks Luise Brand for overseeing the production of the MASC annual report 2014/2015. MASC thanks the German Science Foundation (DFG) for providing the funds to publish this report. 2 | MASC Report 2014/15 TOC Table of Contents Foreword to the Report 5 Executive Summary 7 Progress and Activities of MASC 9 Analysis and Recommendations by MASC 11 Reports of the MASC Subcommittees 15 Bioinformatics _______________________________________________________________________________ 15 Clone-Based Functional Genomics Resources (ORFeomics) ___________________________________________ 16 Epigenenetics and Epigenomics ������������������������������������������������������������������ 18 Metabolomics ________________________________________________________________________________ 19 Natural Variation and Comparative Genomics ______________________________________________________ 20 Phenomics __________________________________________________________________________________ 23 Proteomics __________________________________________________________________________________ 27 Systems and Synthetic Biology ������������������������������������������������������������������� 28 Arabidopsis Community Projects and Resources 31 Resource and Stock Centers ��������������������������������������������������������������������� 32 Arabidopsis Informatics and Data Sharing Resources _________________________________________________ 33 Plant Projects and Resources with Strong Participation of Arabidopsis Community _________________________ 36 Arabidopsis Basic Research and its Impact on Applied Research 39 Scientific Highlights in 2014/15 __________________________________________________________________ 39 Impact on Applied Research and Industry _________________________________________________________ 43 Country Reports of the International Arabidopsis Community 47 Country Highlights ��������������������������������������������������������������������������� 47 Argentina ___________________________________________________________________________________ 49 Austria _____________________________________________________________________________________ 50 Belgium ____________________________________________________________________________________ 52 Canada _____________________________________________________________________________________ 54 Czech Republic _______________________________________________________________________________ 54 Denmark ___________________________________________________________________________________ 56 Finland _____________________________________________________________________________________ 57 France ______________________________________________________________________________________ 58 Germany ___________________________________________________________________________________ 60 Greece _____________________________________________________________________________________ 62 India _______________________________________________________________________________________ 63 Israel _______________________________________________________________________________________ 66 Italy _______________________________________________________________________________________ 67 Japan _______________________________________________________________________________________ 69 Netherlands _________________________________________________________________________________ 71 3 | MASC Report 2014/15 TOC New Zealand ________________________________________________________________________________ 72 South Korea _________________________________________________________________________________ 74 Spain_______________________________________________________________________________________ 75 Sweden _____________________________________________________________________________________ 76 Switzerland __________________________________________________________________________________ 77 United Kingdom _____________________________________________________________________________ 79 United States ________________________________________________________________________________ 82 Members of the MASC Subcommittees 85 Members of the Multinational Arabidopsis Steering Committee 87 4 | MASC Report 2014/15 TOC Foreword to the Report The Multinational Arabidopsis Steering Committee (MASC) ff.), the major Arabidopsis projects and resources