OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AP20144PU-N Polyclonal Antibody to CYP7B1 (C-term) - Aff - Purified Alternate names: 25-hydroxycholesterol 7-alpha-hydroxylase, Cytochrome P450 7B1, Oxysterol 7-alpha- hydroxylase Quantity: 0.1 mg Concentration: 0.5 mg/ml Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. P450 cholesterol 7-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. CYP7B1 (oxysterol 7-α-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25-and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure. Uniprot ID: O75881 NCBI: NP_004811 GeneID: 9420 Host: Goat Immunogen: Peptide with sequence C-YPDSDVLFRYKVKS, from the C Terminus of the protein sequence according to NP_004811.1. Format: State: Liquid purified IgG fraction. Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide. Buffer System: Tris saline, pH~7.3 Preservatives: 0.02% Sodium Azide Stabilizers: 0.5% BSA Applications: Peptide ELISA: Detection Limit: 1/128000. Western blot: Preliminary experiments in Human Liver, Kidney and Prostate lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). Immunohistochemistry on Paraffin Sections: 3-6 µg/ml. In Paraffin Embedded Human Breast shows textured Cytoplasm staining in ductal epithelial cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognizes CYP7B1. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20150416 1 / 2 AP20144PU-N: Polyclonal Antibody to CYP7B1 (C-term) - Aff - Purified Species Reactivity: Tested: Human. Expected from sequence similarity: Bovine Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Wu Z, Martin KO, Javitt NB, Chiang JY. Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1. J Lipid Res. 1999 Dec;40(12):2195-203. PubMed PMID: 10588945. Pictures: Immunohistochemistry: AP20144PU-N CYP7B1 Antibody staining of Paraffin Embedded Human Breast at 4 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP- For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20150416 2 / 2 Powered by TCPDF (www.tcpdf.org).