10th Annual PEGS Europe 2018 Protein & Antibody Engineering Summit Abstracts Lisbon , Portugal 12 - 16 November 2018 ISBN: 978-1-5108-7883-9 Printed from e-media with permission by: Curran Associates, Inc. 57 Morehouse Lane Red Hook, NY 12571 Some format issues inherent in the e-media version may also appear in this print version. Copyright© (2018) by Cambridge EnerTech All rights reserved. Printed with permission by Curran Associates, Inc. (2020) For permission requests, please contact Cambridge EnerTech at the address below. Cambridge EnerTech Cambridge Innovation institute 250 First Avenue Suite 300 Needham, MA 02494 USA Phone: 781-972-5400 Fax: 781-972-5425 [email protected] Additional copies of this publication are available from: Curran Associates, Inc. 57 Morehouse Lane Red Hook, NY 12571 USA Phone: 845-758-0400 Fax: 845-758-2633 Email: [email protected] Web: www.proceedings.com TABLE OF CONTENTS NOVEL FUNCTIONAL STRATEGY FOR DEVELOPMENT OF HIGHLY SPECIFIC ANTIBODY DRUG CONJUGATES FOR NON-HODGKIN'S LYMPHOMA ................................................. 1 A. Andre, J. Dias, S. Aguiar, S. Oliveira, J. Ministro, L. Gano, J. Correia, L. Tavares, F. Aires-da-Silva MAX RANDOMISATION: DESIGNED, NON-DEGENERATE SATURATION MUTAGENESIS OF ARMADILLO REPEAT PROTEINS ............................................................................... 3 A. Chembath, M. Ashraf, B.P.G. Wagstaffe, Y. Stark, A. Pluckthun, A.V. Hine NOVEL MULTIMERS OF BICYCLIC PEPTIDES CLUSTER AND ACTIVATE CD137 (4- 1BB): A COSTIMULATORY T CELL CHECKPOINT RECEPTOR ................................................................ 4 L. Chen, J. Kristensson, G. Mudd, H. Harrison, R. Lani, K. McDonnell, P. Upadhyaya, F. An, J. Lahdenranta, P. Park, N. Keen, K. Hurov INFLUENCE OF CULTURE MEDIA COMPONENTS ON ANTIBODY GLYCOSYLATION PATTERN ............................................................................................................................................................ 5 D. Choi, W. Kang, J. Lee, G.H. Lee, J.H. Kim, W.S. Choi ABDURIN DRUG CONJUGATES: SMALL IN SIZE AND LONG HALF-LIFE TO IMPROVE LIFE TO IMPROVE ............................................................................................................................................ 6 J.W. Choi TARGETED DELIVERY OF ENDOSOMAL TOLL-LIKE RECEPTOR AGONISTS TO THE TUMOUR MICROENVIRONMENT FOR THE PROMOTION OF ANTI-TUMOUR IMMUNITY ......................................................................................................................................................... 7 D. Corogeanu, A. Beavil, S.N. Karagiannis, J.N. Arnold, S.S. Diebold DEVELOPMENT OF A CAPILLARY ISOELECTRIC FOCUSING (CIEF) METHOD FOR CHARGE ANALYSIS OF NOVEL BIOTHERAPEUTICS ............................................................................... 8 J. Cummins, N. Farrands, A. Splevins DEVELOPMENT OF A BLOOD-BRAIN BARRIER-PENETRATING ANTICALIN FOR NON- INVASIVE BRAIN DRUG DELIVERY ............................................................................................................. 9 F.C. Deuschle, A. Skerra A NOVEL ULTRA-HIGH THROUGHPUT SCREEN FOR MICROBIAL TRANSGLUTAMINASE .................................................................................................................................. 10 L. Deweid, H. Kolmar AUTOMATED ONE-TO-ONE LINKAGE OF PROTEINS AND PAYLOADS ............................................. 12 I. Dovgan, A. Hentz, O. Koniev, A. Ehkirch, S. Ursuegui, S. Delacroix, M. Riomet, F. Taran, S. Cianferani, S. Kolodych, A. Wagner ISOLATION OF CHICKEN-DERIVED ANTIBODY FRAGMENTS USING YEAST SURFACE DISPLAY ........................................................................................................................................................... 13 A. Elter, J. Grzeschik, S. Hinz, D. Yanakieva, S. Krah, T. Zollmann, G. Schwall, S. Zielonka, H. Kolmar MULTIFUNCTIONAL BIOLOGICS BASED ON IN VIVO MATURED 100% HUMAN VH DOMAINS ......................................................................................................................................................... 14 C. Enever, J. Legg, B. McGuinness, P. Bland-Ward, P. Pack CELL-BASED ASSAYS FOR SCREENING AND CHARACTERISATION OF ANTIBODY- DRUG CONJUGATES ...................................................................................................................................... 15 R. Forfar, S. Leonard, D. Moore, J. Large, C. Fernandes, R. Brown, C. Southern, D. Matthews, L. Friis, D. Taylor HIGH THROUGHPUT SELECTION OF HUMAN ANTIBODIES WITH ENHANCED DEVELOPABILITY PROPERTIES ................................................................................................................. 16 T. Gallagher, C. Sellick, V. Germaschewski, R. Williams, S. Suhr, D. Melvin THE USE OF IN VITRO ASSAYS TO REDUCE THE RISK OF UNWANTED IMMUNOGENICITY AND ACCELERATE CANCER IMMUNOTHERAPY DEVELOPMENT ................ 17 S. Giltaire, J. Lamy, T. Janss, T. Jonckheere, S. Pattijn ABGENESIS: AN INTEGRATED COMPUTATIONAL SOFTWARE EXPLORING NEW DEPTHS IN ANTIBODY AND IMMUNE REPERTOIRE SEQUENCE ANALYSIS ................................... 18 J. Glanville, D. Maurer, S. Youssef, S. Ives, C. Pettus, R. Newland, V. Chou, K. Hervold, C. Smith, G. Day, C. Jones, S. Daraeikia, A. Sanchez, J. Brysting DRUG CONJUGATES BASED ON ENGINEERED AFFIBODY MOLECULES ......................................... 19 T. Graslund, M. Altai, H. Liu, H. Ding, B. Mitran, P.H. Edqvist, V. Tolmachev, A. Orlova NOVEL CELL CULTURE SYSTEM FOR LONG TERM STABLE IGG AND PROTEIN PRODUCTION .................................................................................................................................................. 20 M. Hagihara, S. Ohya, T. Harada, S. Yamada IMPLEMENTATION OF GENETIC CIRCUITS IN THE YEAST SACCHAROMYCES CEREVISIAE FOR TIGHTLY REGULATED GENE EXPRESSION ............................................................ 22 D. Happel, A. Hofmann, A. Christmann, H. Kolmar RAPID ONLINE AND INPROCESS CHARACTERIZATION OF CETUXIMAB FD- GLYCOPROFILE BY DIRECT MONITORING AND CONTROL ................................................................ 23 M. Hedstrom, S. Bjork, D. Erlandsson, M. Westesson, B. Mattiasson PREDICTING PROTEIN-PROTEIN BINDING SITES AND EPITOPE MAPPING ...................................... 25 A. Henry, N. Thorsteinson, A. Ajamian, J. Gunn, P. Labute DEVELOPMENT AND EVALUATION OF T-ZAP2, A NOVEL ANTIBODY-DRUG CONJUGATE FOR THE TREATMENT OF HER2 POSITIVE BREAST CANCER ..................................... 26 R.M. Hoffmann, S. Crescioli, D. Thurston, S. Karagiannis PASYLATED COLCHICALIN - A POSSIBLE ANTIDOTE FOR ACUTE COLCHICINE INTOXICATION WITH OPTIMIZED PHARMACOKINETICS .................................................................... 27 E. Ilyukhina, M. Barkovskiy, M. Dauner, A. Eichinger, J. Stenzel, N. Jung, H. Neuberger, F. Eyer, A. Skerra SELECTION OF SCAFFOLD PROTEINS FOR ANTIBODY MIMETICS .................................................... 28 T. Ito, H. Nishi, T. Kameda, T. Niide, H. Nakazawa, M. Umetsu APPRAISAL OF LEUCINE-RICH ALPHA-2-GLYCOPROTEIN 1 (LRG1) AS A TARGET FOR A POTENTIAL ANTIBODY-DRUG CONJUGATE (ADC) ........................................................................... 29 F. Javaid, S.E. Moss, J. Greenwood, V. Chudasama ANTIBODY-MEDIATED INHIBITION OF PEPTIDYL ARGININE DEIMINASE (PPAD) FROM PORPHYROMONAS GINGIVALIS .................................................................................................... 30 J.Z. Kaczmarek, J.Z. Kaczmarek, D.N. Larsen, B. Szmigielski, J. Potempa, P.D. Skottrup, P. Hojrup DRUG-TARGET-COMPLEX SPECIFIC ANTIBODIES FOR PHARMACOKINETIC ANALYSIS OF BIOTHERAPEUTICS ............................................................................................................. 32 S.J. Kellmann, C. Frisch, S. Harth, A. Knappik APPLICATIONS FOR HYPERIMMUNE, AUTOIMMUNE DIVERSIMAB™ MICE IN ANTIBODY DISCOVERY ............................................................................................................................... 33 R.P. Kelly, B. Bouchard, R. Abdullah, R. Sahoo, R. Subburathinam, A. Bemis, K. Teague, E. Batitay, H. Wagner, J. Gabriel, C. Souders, T. Mullen, G. Hilow, D. Turner OBTAINING OF A PROTEIN WITH MOLECULAR RECOGNITION BY GRAFTING A FUNCTIONAL PEPTIDE SEQUENCE WITH THE SAME SECONDARY STRUCTURE .......................... 34 M. Kikuchi, T. Niide, H. Nakazawa, H. Imanaka, M. Umetsu INTEGRATED EARLY STAGE DEVELOPMENT STRATEGY AS RISK MANAGEMENT TOOL FOR GLOBAL DEVELOPMENT ......................................................................................................... 35 N. Kinsella, J.B. Holz NATURAL AND FULLY HUMAN ANTIBODIES FOR DIAGNOSTICS AND THERAPY ........................ 36 J. Kugler MINIATURIZATION AND OPTIMIZATION OF A TWO-PLASMID EXPRESSION PATHWAY IN MYTXTL® CELL-FREE PROTEIN EXPRESSION KIT (ARBOR BIOSCIENCES) USING LABCYTE ECHO® ACOUSTIC TECHNOLOGY ................................................. 37 A. Lehto, E. Eggenstein, J. Bailey, J.M. Rouillard, J. Lesnick HOS BIOSIMILARITY AND COMPARABILITY: OBJECTIVE, QUANTIFIABLE HOS COMPARISONS - A BIOSIMILAR CASE STUDY UTILIZING CIRCULAR DICHROISM ....................... 38 T. Lester, M. Textor DESIGNING AN ARTIFICIAL GOLGI REACTOR FOR CELL-FREE GLYCOSYLATION ...................... 39 E. Makrydaki, I.M. Ramirez, L. Bouche, S. Haslam, C. Kontoravdi, K.M. Polizzi BINDERS FOR CHALLENGING TASKS - A HIGH-THROUGHPUT DARPIN SELECTION PIPELINE ..........................................................................................................................................................