SAN TA C RUZ BI OTEC HNOL OG Y, INC . TREML3 (T-15): sc-324449 BACKGROUND PRODUCT TREML3 (triggering receptor expressed on myeloid cells-like 3), also known Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide as TLT3, is a 195 amino acid single-pass type I membrane protein that con tains and 0.1% gelatin. one Ig-like V-type domain. The gene encoding TREML3 is located within a Blocking peptide available for competition studies, sc-324449 P, (100 µg TREM cluster on chromosome 6 that contains two other TREML proteins, pep tide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). namely TREML1 and TREML2. Chromosome 6 contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the APPLICATIONS q arm of chromosome 6 is associated with early onset intestinal cancer, sug - gesting the presence of a cancer susceptibility locus. Additionally, Porphyria TREML3 (T-15) is recommended for detection of TREML3 of human origin cutanea tarda, Parkinson’s disease, Stickler syndrome and a susceptibility to by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), bipolar disorder are all associated with genes that map to chromosome 6. immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000); non REFERENCES cross-reactive with TREML1, TREML2 or TREML4. 1. Online Mendelian Inheritance in Man, OMIM™. 2002. Johns Hopkins Suitable for use as control antibody for TREML3 siRNA (h): sc-95288, TREML3 University, Baltimore, MD. MIM Number: 609716. World Wide Web URL: shRNA Plasmid (h): sc-95288-SH and TREML3 shRNA (h) Lentiviral Particles: http://www.ncbi.nlm.nih.gov/omim/ sc-95288-V. 2. Allcock, R.J., Barrow, A.D., Forbes, S., Beck, S. and Trowsdale, J. 2003. Molecular Weight of TREML3: 18 kDa. The human TREM gene cluster at 6p21.1 encodes both activating and inhibitory single IgV domain receptors and includes NKp44. Eur. J. RECOMMENDED SECONDARY REAGENTS Immunol. 33: 567-577. To ensure optimal results, the following support (secondary) reagents are 3. Mungall, A.J., Palmer, S.A., Sims, S.K., Edwards, C.A., Ashurst, J.L., recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 Wilming, L., Jones, M.C., Horton, R., Hunt, S.E., Scott, C.E., Gilbert, J.G.R., (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey Clamp, M.E., Bethel, G., Milne, S., Ainscough, R., Almeida, J.P., Ambrose, anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ K.D., et al. 2003. The DNA sequence and analysis of human chromosome 6. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: Nature 425: 805-811. sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- 4. Vuoristo, M.M., Pappas, J.G., Jansen, V. and Ala-Kokko, L. 2004. A stop 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) codon mutation in COL11A2 induces exon skipping and leads to non-ocu lar with UltraCruz™ Mounting Medium: sc-24941. Stickler syndrome. Am. J. Med. Genet. A 130: 160-164. 5. McQueen, M.B., Devlin, B., Faraone, S.V., Nimgaonkar, V.L., Sklar, P., STORAGE Smoller, J.W., Abou Jamra, R., Albus, M., et al. 2005. Combined analysis Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of from eleven linkage studies of bipolar disorder provides strong evidence shipment. Non-hazardous. No MSDS required. of susceptibility loci on chromosomes 6q and 8q. Am. J. Hum. Genet. 77: 582-595. RESEARCH USE 6. Safadi, S.S. and Shaw, G.S. 2007. A disease state mutation unfolds the For research use only, not for use in diagnostic procedures. parkin ubiquitin-like domain. Biochemistry 46: 14162-14169. 7. Bläker, H., Mechtersheimer, G., Sutter, C., Hertkorn, C., Kern, M.A., Rieker, PROTOCOLS R.J., Penzel, R., Schirmacher, P. and Kloor, M. 2008. Recurrent deletions at See our web site at www.scbt.com or our catalog for detailed protocols and 6q in early age of onset non-HNPCC- and non-FAP-associated intestinal support products. carcinomas. Evidence for a novel cancer susceptibility locus at 6q14-q22. Genes Chromosomes Cancer 47: 159-164. CHROMOSOMAL LOCATION Genetic locus: TREML3 (human) mapping to 6p21.1. SOURCE TREML3 (T-15) is an affinity purified goat polyclonal antibody raised against a peptide mapping near the N-terminus of TREML3 of human origin. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.